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The map label for this gene is ddl

Identifier: 170748771

GI number: 170748771

Start: 2489125

End: 2490048

Strand: Reverse

Name: ddl

Synonym: Mrad2831_2353

Alternate gene names: 170748771

Gene position: 2490048-2489125 (Counterclockwise)

Preceding gene: 170748772

Following gene: 170748770

Centisome position: 40.97

GC content: 66.45

Gene sequence:

>924_bases
ATGTCCAAGCACGTCGCCGTCCTCCTGGGCGGCACCTCCGCCGAGCGCGAGGTCTCCCTCAATTCCGGCAAGGCCTGCGC
CGACGCGCTCGAAGGGGAGGGCTACCGGGTCACCCGGGTCGATGTCGGCCCCGACATCGCCTCCGTGCTGACGGCGCTCC
GGCCGGACGCGGCGTTCAACGCGCTCCACGGACCTGACGGCGAGGACGGCACGATCCAGGGCCTGCTGGAGATCCTCAAG
ATCCCCTACACCCATTCCGGCGTCCTCGCCTCGGCGCTGGCCATGAACAAGGAGCGGGCCAAGACCGTCATGCGCGCCGC
CGGCGTCGACGTGCCGGAGGGCCGGATCGTTAACCGTCGTGAGGCCGCCAGGACACACCCCCTGCCGCCACCCTACGTGG
TGAAACCGATCGCCGAAGGCTCATCGGTGGGTGTGATCATCGTGCGCGACGGCCGCTCGCACCCACCCCAGATCCTCGCC
TCCGAGGAATGGACGTTCGGCGAGCAAGTCCTTGCAGAACCTTACATTGCTGGCCGCGAACTCACCTGCGGCGTGATGGG
CGACAAGGCCCTCGGGGTGATCGAGGTGAAGGCCGCCACGGGGGATTGGTACGATTACGACGCCAAGTACGCTCCGGGGG
GCTCGGTTCACGTGCTCCCGGCCGAACTTAAACCAAATGTTTACCAGCGTGTCCAAGAACTGTCGTTAACGGCGCATCAA
GCACTGGGCTGTCGGGGCGTCAGCCGTGCCGACCTTCGCTACGACGACACACCGGGTGGAACCGGTCTGCTCGTGGTGCT
GGAGGTCAACACGCAACCCGGCATGACCCAGACGAGCCTTGTGCCGGAGATGGCGGCCCATGCGGGCCTGAGTTTCGGTG
AGCTCGTCCGATGGATGGTGGAGGACGCTTCCCTGAATCGCTGA

Upstream 100 bases:

>100_bases
CGGACGCGCCGACGCGGCCGAGATACGATGCTCGGCCGGCGCAGCGACCGTTCGGGTGCGTTCAGATCCTGATTGAAGCC
GACCTTTTCGAGGTGCCCTG

Downstream 100 bases:

>100_bases
GCGGCGACGCCGCCGGCCGGAGCGGTCCCGCGCGCCTCGCCGGCGCGATCCTGGGCCGGCTCGCCGGCCGCGCCACGTCC
CGGTCGCTCTCGGTTCGTCG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSKHVAVLLGGTSAEREVSLNSGKACADALEGEGYRVTRVDVGPDIASVLTALRPDAAFNALHGPDGEDGTIQGLLEILK
IPYTHSGVLASALAMNKERAKTVMRAAGVDVPEGRIVNRREAARTHPLPPPYVVKPIAEGSSVGVIIVRDGRSHPPQILA
SEEWTFGEQVLAEPYIAGRELTCGVMGDKALGVIEVKAATGDWYDYDAKYAPGGSVHVLPAELKPNVYQRVQELSLTAHQ
ALGCRGVSRADLRYDDTPGGTGLLVVLEVNTQPGMTQTSLVPEMAAHAGLSFGELVRWMVEDASLNR

Sequences:

>Translated_307_residues
MSKHVAVLLGGTSAEREVSLNSGKACADALEGEGYRVTRVDVGPDIASVLTALRPDAAFNALHGPDGEDGTIQGLLEILK
IPYTHSGVLASALAMNKERAKTVMRAAGVDVPEGRIVNRREAARTHPLPPPYVVKPIAEGSSVGVIIVRDGRSHPPQILA
SEEWTFGEQVLAEPYIAGRELTCGVMGDKALGVIEVKAATGDWYDYDAKYAPGGSVHVLPAELKPNVYQRVQELSLTAHQ
ALGCRGVSRADLRYDDTPGGTGLLVVLEVNTQPGMTQTSLVPEMAAHAGLSFGELVRWMVEDASLNR
>Mature_306_residues
SKHVAVLLGGTSAEREVSLNSGKACADALEGEGYRVTRVDVGPDIASVLTALRPDAAFNALHGPDGEDGTIQGLLEILKI
PYTHSGVLASALAMNKERAKTVMRAAGVDVPEGRIVNRREAARTHPLPPPYVVKPIAEGSSVGVIIVRDGRSHPPQILAS
EEWTFGEQVLAEPYIAGRELTCGVMGDKALGVIEVKAATGDWYDYDAKYAPGGSVHVLPAELKPNVYQRVQELSLTAHQA
LGCRGVSRADLRYDDTPGGTGLLVVLEVNTQPGMTQTSLVPEMAAHAGLSFGELVRWMVEDASLNR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=313, Percent_Identity=39.9361022364217, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=30.5714285714286, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_METRJ (B1LXZ1)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001755031.1
- ProteinModelPortal:   B1LXZ1
- SMR:   B1LXZ1
- GeneID:   6138385
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_2353
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32636; Mature: 32504

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHVAVLLGGTSAEREVSLNSGKACADALEGEGYRVTRVDVGPDIASVLTALRPDAAFN
CCCEEEEEEECCCCCEEEECCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCHHHH
ALHGPDGEDGTIQGLLEILKIPYTHSGVLASALAMNKERAKTVMRAAGVDVPEGRIVNRR
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEECCH
EAARTHPLPPPYVVKPIAEGSSVGVIIVRDGRSHPPQILASEEWTFGEQVLAEPYIAGRE
HHHHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCCCCE
LTCGVMGDKALGVIEVKAATGDWYDYDAKYAPGGSVHVLPAELKPNVYQRVQELSLTAHQ
EEEEECCCCCEEEEEEEECCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ALGCRGVSRADLRYDDTPGGTGLLVVLEVNTQPGMTQTSLVPEMAAHAGLSFGELVRWMV
HHCCCCCCCCCCEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
EDASLNR
HHHCCCC
>Mature Secondary Structure 
SKHVAVLLGGTSAEREVSLNSGKACADALEGEGYRVTRVDVGPDIASVLTALRPDAAFN
CCEEEEEEECCCCCEEEECCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCHHHH
ALHGPDGEDGTIQGLLEILKIPYTHSGVLASALAMNKERAKTVMRAAGVDVPEGRIVNRR
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEECCH
EAARTHPLPPPYVVKPIAEGSSVGVIIVRDGRSHPPQILASEEWTFGEQVLAEPYIAGRE
HHHHCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCCCCE
LTCGVMGDKALGVIEVKAATGDWYDYDAKYAPGGSVHVLPAELKPNVYQRVQELSLTAHQ
EEEEECCCCCEEEEEEEECCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ALGCRGVSRADLRYDDTPGGTGLLVVLEVNTQPGMTQTSLVPEMAAHAGLSFGELVRWMV
HHCCCCCCCCCCEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
EDASLNR
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA