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The map label for this gene is pleD [H]

Identifier: 170748282

GI number: 170748282

Start: 1993424

End: 1994797

Strand: Reverse

Name: pleD [H]

Synonym: Mrad2831_1864

Alternate gene names: 170748282

Gene position: 1994797-1993424 (Counterclockwise)

Preceding gene: 170748283

Following gene: 170748281

Centisome position: 32.82

GC content: 71.83

Gene sequence:

>1374_bases
ATGTCGGCCCGTGTCCTGATCGTCGACGATCTGTTCCCGAACGTCAGGCTTCTGGAGACGAAGCTCGGCCTGGAATATTT
CGACACGCTCGCGGCCATGAACGGCCGGGACGCGATCGCGATCTGCGAGAAGGGCCTCTGCGACCTCGTCCTGCTCGACG
TGATGATGCCCGGCATGGACGGGTTCGAGGTCTGCCGGCACCTCAAGAACAACCCGCTCACGGCCCATATCCCGGTGGTG
ATGGTCACGGCCCTCGACCAGCCCGCGGACCGCCTGCGCGGCCTCGATGCCGGGGCCGACGACTTCCTGACCAAGCCGGT
GGACGACACCGCCCTGTTCACGCGGGTGCGCAGCCTGGTGCGGCTCAAGGCCGTGACGGACGAGCTGCGCCAGCGCGCGC
TCGCCTCCCGCGAGTTCGGCATCGGCGATCCCCTGGCGCTCGCCACCGCCGAGACCGGCCTCGACGCCCGGATCCTGCTG
ATCGAGGACCGTCCCGGATCGGCCGAGCGCCTCGCCGGGGCCCTGCGCCAGCACCACGTCGTCACGGTCGAGCCCGACCC
GCAGGAGGCCCTGCGCCGCGCCGCCGAGGAGACCTTCGACCTCGCCCTGGTGAGCCTCGACCTCACCGGCTTCGACGGCC
TGCGCCTGTGCAGCCAGCTCCGGTCCCTCGACCGGACCCGGGCGATGCCGCTGATCATGCTCGCCGAGGAGTACGACCGC
GCCCGGGTGGTCCGCGGCCTCGATTTCGGCGTGCACGACTTCCTGATGCGGCCGGTCGACCGCAACGAGCTGATGGCCCG
GGTCCGCACGCAGGTGAAGCGCAAGCGCTTCACCGACGCGCTCCGCGGGGCGATGCAGGCCTCCCTGCAGATGGCCGTGA
CCGATGCGCTCACCGGCCTGCACAACCGGCGCTACCTCGACAACCACCTCGGCACGCTGTTCGGCGACGAGGCCGCCCGC
CGGGCGAACCTCGCCGCGCTGATCCTCGACATCGACCACTTCAAGGGGATCAACGACAGCTTCGGCCACGAGGCCGGCGA
CGAGGTGCTGCGCGGCTTCGCCGAGCGCGTCCGCCAGCACACGCGGCCCATCGACATCGTGGCGCGCTACGGCGGCGAGG
AGGTGGTGGTGATCCTGCCGGAGGCGGGCCTCGTCGAGGCGCAGGGCATAGCCGAGCGCATCCGCGAGCGCGTCGAGGCG
GTGCCGTTCACCGTGCAGCGGGCGAGCCGGACCGTGTCGGTGACGGTCTCGATCGGCGTCGCCGTGCGCCGCGGCGACGA
TCTCGGCCCCGCCGACATGCTCCGCCGGGCCGACCTCGCCCTCTACCGGGCCAAGGCGGCGGGCCGGAACCGGGTCGAGT
CGCAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CCCGTCGTGTCCGCCCCGGCCCCGGCGGCCGACCGGCGGCGCGCACCCACGGCACACGGCGCCCGGTCCGGGCCGCTCAC
CCGCGCCCTGACGAGGAAGC

Downstream 100 bases:

>100_bases
CCGGCGGCCTGACAGGAGTCCCCCCGCGTCAATCCGCGCGCCCGCGCGCGCGGATGAAAAGGCCGGCCTCGCCGTGTTAT
CGGTTCGGCCGGCCCGGCGA

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSARVLIVDDLFPNVRLLETKLGLEYFDTLAAMNGRDAIAICEKGLCDLVLLDVMMPGMDGFEVCRHLKNNPLTAHIPVV
MVTALDQPADRLRGLDAGADDFLTKPVDDTALFTRVRSLVRLKAVTDELRQRALASREFGIGDPLALATAETGLDARILL
IEDRPGSAERLAGALRQHHVVTVEPDPQEALRRAAEETFDLALVSLDLTGFDGLRLCSQLRSLDRTRAMPLIMLAEEYDR
ARVVRGLDFGVHDFLMRPVDRNELMARVRTQVKRKRFTDALRGAMQASLQMAVTDALTGLHNRRYLDNHLGTLFGDEAAR
RANLAALILDIDHFKGINDSFGHEAGDEVLRGFAERVRQHTRPIDIVARYGGEEVVVILPEAGLVEAQGIAERIRERVEA
VPFTVQRASRTVSVTVSIGVAVRRGDDLGPADMLRRADLALYRAKAAGRNRVESQAA

Sequences:

>Translated_457_residues
MSARVLIVDDLFPNVRLLETKLGLEYFDTLAAMNGRDAIAICEKGLCDLVLLDVMMPGMDGFEVCRHLKNNPLTAHIPVV
MVTALDQPADRLRGLDAGADDFLTKPVDDTALFTRVRSLVRLKAVTDELRQRALASREFGIGDPLALATAETGLDARILL
IEDRPGSAERLAGALRQHHVVTVEPDPQEALRRAAEETFDLALVSLDLTGFDGLRLCSQLRSLDRTRAMPLIMLAEEYDR
ARVVRGLDFGVHDFLMRPVDRNELMARVRTQVKRKRFTDALRGAMQASLQMAVTDALTGLHNRRYLDNHLGTLFGDEAAR
RANLAALILDIDHFKGINDSFGHEAGDEVLRGFAERVRQHTRPIDIVARYGGEEVVVILPEAGLVEAQGIAERIRERVEA
VPFTVQRASRTVSVTVSIGVAVRRGDDLGPADMLRRADLALYRAKAAGRNRVESQAA
>Mature_456_residues
SARVLIVDDLFPNVRLLETKLGLEYFDTLAAMNGRDAIAICEKGLCDLVLLDVMMPGMDGFEVCRHLKNNPLTAHIPVVM
VTALDQPADRLRGLDAGADDFLTKPVDDTALFTRVRSLVRLKAVTDELRQRALASREFGIGDPLALATAETGLDARILLI
EDRPGSAERLAGALRQHHVVTVEPDPQEALRRAAEETFDLALVSLDLTGFDGLRLCSQLRSLDRTRAMPLIMLAEEYDRA
RVVRGLDFGVHDFLMRPVDRNELMARVRTQVKRKRFTDALRGAMQASLQMAVTDALTGLHNRRYLDNHLGTLFGDEAARR
ANLAALILDIDHFKGINDSFGHEAGDEVLRGFAERVRQHTRPIDIVARYGGEEVVVILPEAGLVEAQGIAERIRERVEAV
PFTVQRASRTVSVTVSIGVAVRRGDDLGPADMLRRADLALYRAKAAGRNRVESQAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=176, Percent_Identity=37.5, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=39.6341463414634, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=41.6666666666667, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=181, Percent_Identity=40.3314917127072, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=35.1190476190476, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=37.6470588235294, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081881, Length=191, Percent_Identity=38.7434554973822, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=39.5061728395062, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=34.7826086956522, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=37.1900826446281, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789809, Length=164, Percent_Identity=31.0975609756098, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082012, Length=91, Percent_Identity=41.7582417582418, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788394, Length=139, Percent_Identity=35.2517985611511, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787541, Length=164, Percent_Identity=30.4878048780488, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=129, Percent_Identity=34.1085271317829, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=178, Percent_Identity=30.3370786516854, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=33.8983050847458, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=33.9622641509434, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790863, Length=143, Percent_Identity=28.6713286713287, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790860, Length=120, Percent_Identity=36.6666666666667, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=38.8349514563107, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081974, Length=109, Percent_Identity=31.1926605504587, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI2367329, Length=192, Percent_Identity=32.8125, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50504; Mature: 50373

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARVLIVDDLFPNVRLLETKLGLEYFDTLAAMNGRDAIAICEKGLCDLVLLDVMMPGMD
CCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCCC
GFEVCRHLKNNPLTAHIPVVMVTALDQPADRLRGLDAGADDFLTKPVDDTALFTRVRSLV
HHHHHHHHCCCCCEEECCEEEEECCCCHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHH
RLKAVTDELRQRALASREFGIGDPLALATAETGLDARILLIEDRPGSAERLAGALRQHHV
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCE
VTVEPDPQEALRRAAEETFDLALVSLDLTGFDGLRLCSQLRSLDRTRAMPLIMLAEEYDR
EEECCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHH
ARVVRGLDFGVHDFLMRPVDRNELMARVRTQVKRKRFTDALRGAMQASLQMAVTDALTGL
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNRRYLDNHLGTLFGDEAARRANLAALILDIDHFKGINDSFGHEAGDEVLRGFAERVRQH
HCCHHHHHHHHHHHCCHHHHHHCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
TRPIDIVARYGGEEVVVILPEAGLVEAQGIAERIRERVEAVPFTVQRASRTVSVTVSIGV
CCCEEEHEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEEE
AVRRGDDLGPADMLRRADLALYRAKAAGRNRVESQAA
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
SARVLIVDDLFPNVRLLETKLGLEYFDTLAAMNGRDAIAICEKGLCDLVLLDVMMPGMD
CCEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCCC
GFEVCRHLKNNPLTAHIPVVMVTALDQPADRLRGLDAGADDFLTKPVDDTALFTRVRSLV
HHHHHHHHCCCCCEEECCEEEEECCCCHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHH
RLKAVTDELRQRALASREFGIGDPLALATAETGLDARILLIEDRPGSAERLAGALRQHHV
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCE
VTVEPDPQEALRRAAEETFDLALVSLDLTGFDGLRLCSQLRSLDRTRAMPLIMLAEEYDR
EEECCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHH
ARVVRGLDFGVHDFLMRPVDRNELMARVRTQVKRKRFTDALRGAMQASLQMAVTDALTGL
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNRRYLDNHLGTLFGDEAARRANLAALILDIDHFKGINDSFGHEAGDEVLRGFAERVRQH
HCCHHHHHHHHHHHCCHHHHHHCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
TRPIDIVARYGGEEVVVILPEAGLVEAQGIAERIRERVEAVPFTVQRASRTVSVTVSIGV
CCCEEEHEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEEE
AVRRGDDLGPADMLRRADLALYRAKAAGRNRVESQAA
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA