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The map label for this gene is puuD [H]

Identifier: 170748249

GI number: 170748249

Start: 1957750

End: 1958535

Strand: Reverse

Name: puuD [H]

Synonym: Mrad2831_1831

Alternate gene names: 170748249

Gene position: 1958535-1957750 (Counterclockwise)

Preceding gene: 170748250

Following gene: 170748248

Centisome position: 32.22

GC content: 72.9

Gene sequence:

>786_bases
ATGACGAGACCCGTGATCGGCGTCATCGGCAACGCGCGCCTGATCGACAGCCGCTTCCCGGCACAGATCGTCGGCGAGAA
CAACATGCGGGCGGTCGCCGCGGTGGCCGGCGCCCTGCCGCTGATGTTCGCCGCCGTCCCGGCGATCACCGACCTCGGCG
ACCTCCTCGACACCGTGGACGGCGTCCTGCTCACCGGGGGCCGCGCCAACGTCCACCCGGCGCGCTTCAACACCGAGCCC
TGCGCGGCGCACGAGCCCTACGACGAGGATCGCGACGCCGTGGCGCTGCCGCTGATCGAGGCCTGCGTGGCGCGCGGCGT
GCCGCTGTTCGGCATCTGCCGCGGCTTCCAGGAGATGAACGTCGCCTTCGGGGGCAGCCTGCACCCGGAGATCCGCGATC
TGCCGGGCCGGCTCAACCACCGCATGCCGCGCCTCGAGAACGGCGAGATCCACCCGGACCTGGAACTGATCTTCGCCGAC
CGTCACGAGGTCAGGCTGGCGCCCGGGGGCACCTTCGCCCGCCTCCTGGAGCGCGAGACGATCCGGGTGAACTCCCTGCA
CGGCCAAGGTATTCTGGAGCCGGGCGGGCGGATCGTGGTCGAGGGCATCGCCGAGGACGAGACGGTCGAGGCGATCCGCG
TCCGCGACGCGGCGGACTTCGCGCTGGGCGTGCAGTGGCACGCCGAGCACGACCCGGCGACCAACCCGATCAACCGCGCC
CTGTTCGAGGCCTTCGGGGCCGCCGCGGCGACGCGGCACCGGGCGCGAACGGCCGGGCGGGCCTGA

Upstream 100 bases:

>100_bases
CCGCATAGGACTGGCGCCGGCCTTGCGTGGGTCGTAGAGCCTGCCGCGGAGCGCGGCGGCCGCCGCGCGGCCAGGACAGG
ATCGAGGAGCAGCCGGGACG

Downstream 100 bases:

>100_bases
GCTTCCACCCGGCGGATCGGCCCAGTCCAGGCGGCCCTCGCGGCAGCACAGCGCCGGAGCGGTGCCAGTCCGGCGAGTCT
TTCCTGCCTTAACCTATGTT

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTRPVIGVIGNARLIDSRFPAQIVGENNMRAVAAVAGALPLMFAAVPAITDLGDLLDTVDGVLLTGGRANVHPARFNTEP
CAAHEPYDEDRDAVALPLIEACVARGVPLFGICRGFQEMNVAFGGSLHPEIRDLPGRLNHRMPRLENGEIHPDLELIFAD
RHEVRLAPGGTFARLLERETIRVNSLHGQGILEPGGRIVVEGIAEDETVEAIRVRDAADFALGVQWHAEHDPATNPINRA
LFEAFGAAAATRHRARTAGRA

Sequences:

>Translated_261_residues
MTRPVIGVIGNARLIDSRFPAQIVGENNMRAVAAVAGALPLMFAAVPAITDLGDLLDTVDGVLLTGGRANVHPARFNTEP
CAAHEPYDEDRDAVALPLIEACVARGVPLFGICRGFQEMNVAFGGSLHPEIRDLPGRLNHRMPRLENGEIHPDLELIFAD
RHEVRLAPGGTFARLLERETIRVNSLHGQGILEPGGRIVVEGIAEDETVEAIRVRDAADFALGVQWHAEHDPATNPINRA
LFEAFGAAAATRHRARTAGRA
>Mature_260_residues
TRPVIGVIGNARLIDSRFPAQIVGENNMRAVAAVAGALPLMFAAVPAITDLGDLLDTVDGVLLTGGRANVHPARFNTEPC
AAHEPYDEDRDAVALPLIEACVARGVPLFGICRGFQEMNVAFGGSLHPEIRDLPGRLNHRMPRLENGEIHPDLELIFADR
HEVRLAPGGTFARLLERETIRVNSLHGQGILEPGGRIVVEGIAEDETVEAIRVRDAADFALGVQWHAEHDPATNPINRAL
FEAFGAAAATRHRARTAGRA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=254, Percent_Identity=37.4015748031496, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 28121; Mature: 27990

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVIGVIGNARLIDSRFPAQIVGENNMRAVAAVAGALPLMFAAVPAITDLGDLLDTVD
CCCCEEEECCCCEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVLLTGGRANVHPARFNTEPCAAHEPYDEDRDAVALPLIEACVARGVPLFGICRGFQEMN
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHH
VAFGGSLHPEIRDLPGRLNHRMPRLENGEIHPDLELIFADRHEVRLAPGGTFARLLERET
HHCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHH
IRVNSLHGQGILEPGGRIVVEGIAEDETVEAIRVRDAADFALGVQWHAEHDPATNPINRA
EEEEEECCCCEECCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHH
LFEAFGAAAATRHRARTAGRA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRPVIGVIGNARLIDSRFPAQIVGENNMRAVAAVAGALPLMFAAVPAITDLGDLLDTVD
CCCEEEECCCCEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVLLTGGRANVHPARFNTEPCAAHEPYDEDRDAVALPLIEACVARGVPLFGICRGFQEMN
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHH
VAFGGSLHPEIRDLPGRLNHRMPRLENGEIHPDLELIFADRHEVRLAPGGTFARLLERET
HHCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHH
IRVNSLHGQGILEPGGRIVVEGIAEDETVEAIRVRDAADFALGVQWHAEHDPATNPINRA
EEEEEECCCCEECCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHH
LFEAFGAAAATRHRARTAGRA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]