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The map label for this gene is gatA [H]

Identifier: 170748139

GI number: 170748139

Start: 1830313

End: 1831596

Strand: Reverse

Name: gatA [H]

Synonym: Mrad2831_1721

Alternate gene names: 170748139

Gene position: 1831596-1830313 (Counterclockwise)

Preceding gene: 170748140

Following gene: 170748138

Centisome position: 30.14

GC content: 76.32

Gene sequence:

>1284_bases
ATGAGCGTACGCGAGACCGTCGAGGAGCGTCTGGCCCGGATCGCCGAGGGGGACCCGGCGGTCTTCACCCGCCTGTACGC
GGAACAGGCCCGCGCGGCGGCGGACGCCGCCGATGCGCGCCGCCGGGACGGGATCTCCCTCGGCCCCCTCGACGGCGCGA
TGGTCTCGATCAAGGACCTGTTCGACGTGGCGGGCGAGACGACGCGCGCCGGCTCGATCCTGCTCGCCGACGCGGCGCCC
GCGCGGGCCGACGCCCCGGTGGTCGCCCGGCTGCGCCGGGCCGGCGCGGTGATCCTGGGCAAGACCAACATGTCCGAGTT
CGCCTTCTCGGGGCTCGGCCTCAACCCGCATTACGGCACCCCCGGCAACGCCGCCGATCCGGCGCGCATCCCCGGGGGCT
CGTCCTCCGGCGCCGGGGTCTCGGTGGGGCAGGGGACGAGCGACATCGCGATCGGGTCCGACACGGGCGGCTCCGTCCGG
ATCCCGGCCGCGCTGAACGGCGTCGTCGGCTTCAAGCCGACCGCAAGCCGCGTGCCGCTGGAGGGAGCGTTCCCGCTGTC
CTACGCCCTCGATTCCCTGGGGCCCCTCGCCCGGACGGTCGCGGATTGCGCGGCCGCCGACGCCGTGATGGCGGGCGAGG
AGCCCGCGCCGCTGGAGGCGCTCGCCGTCGCCGGCCTGCGCATCGGCGTGCCGCGGGGCCTGCTGTTCACCGAGACGGAA
GGGCCTGTCGCCGAGGCCTTCGAGCGGGTGCTGTCGCGCCTGTCCCGGGCCGGCGCCCGGATCGCCGATCACCCGATCGA
CGACCACCTCGCCGCCATGGACGCGGCGCTCCCGGACGGCTCCCTCGCGGCGATCGAGGCCGCGGCGATCCACGCCGACT
GGCTGGACAGCGCGGCCGACCGCTACGACCCGCGGGTCCATCGCCGCATCGTGGCCGGCCGGGGCGCGACGGCCGCGGGC
TACATCCGGACGATGCGCCGGCGGGCCGCGCTGATCGCCGCCTTCGACCGGTACCTCGCCCCGCTCGACGTGCTGGCGCT
CCCGGCGACCGCCACGACGGCGCCGCTCACCGCCCCGCTGGCGGCGGACGACGCGGCCTTCCTGGCGGCCAACCGCCTGA
TGCTGCGCAACACCGCGTTCGGCAACTTCTTCGATCTCACCGGCCTGTCCCTGCCGATGCCGGACCTGCCCCGGCCGGCG
GGCCTGATGCTGCTGGCCCGGCACGGTCAGGATCGCCGGCTCCTGGCGATCGGGGCCGGGGTCGAGGCCGCGCTGGCGGA
GTGA

Upstream 100 bases:

>100_bases
GAACCTGCTGATGGATGGCGGCGCCTATCCCGGCACGTTCTGACGCGGACCGGCCCTCCGTTTGCAGGGGCGGGCCGGAT
CACATCGTGCGGTGAGGACC

Downstream 100 bases:

>100_bases
GCGATCATCCATCCGCCGGCGCGCCGCCTGTGCAGGTTGCGCGGGACTGCGCCGCGAGCAGTGATGGAGGGGAATACACG
ACGAACCTCGCGCGATGCGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSVRETVEERLARIAEGDPAVFTRLYAEQARAAADAADARRRDGISLGPLDGAMVSIKDLFDVAGETTRAGSILLADAAP
ARADAPVVARLRRAGAVILGKTNMSEFAFSGLGLNPHYGTPGNAADPARIPGGSSSGAGVSVGQGTSDIAIGSDTGGSVR
IPAALNGVVGFKPTASRVPLEGAFPLSYALDSLGPLARTVADCAAADAVMAGEEPAPLEALAVAGLRIGVPRGLLFTETE
GPVAEAFERVLSRLSRAGARIADHPIDDHLAAMDAALPDGSLAAIEAAAIHADWLDSAADRYDPRVHRRIVAGRGATAAG
YIRTMRRRAALIAAFDRYLAPLDVLALPATATTAPLTAPLAADDAAFLAANRLMLRNTAFGNFFDLTGLSLPMPDLPRPA
GLMLLARHGQDRRLLAIGAGVEAALAE

Sequences:

>Translated_427_residues
MSVRETVEERLARIAEGDPAVFTRLYAEQARAAADAADARRRDGISLGPLDGAMVSIKDLFDVAGETTRAGSILLADAAP
ARADAPVVARLRRAGAVILGKTNMSEFAFSGLGLNPHYGTPGNAADPARIPGGSSSGAGVSVGQGTSDIAIGSDTGGSVR
IPAALNGVVGFKPTASRVPLEGAFPLSYALDSLGPLARTVADCAAADAVMAGEEPAPLEALAVAGLRIGVPRGLLFTETE
GPVAEAFERVLSRLSRAGARIADHPIDDHLAAMDAALPDGSLAAIEAAAIHADWLDSAADRYDPRVHRRIVAGRGATAAG
YIRTMRRRAALIAAFDRYLAPLDVLALPATATTAPLTAPLAADDAAFLAANRLMLRNTAFGNFFDLTGLSLPMPDLPRPA
GLMLLARHGQDRRLLAIGAGVEAALAE
>Mature_426_residues
SVRETVEERLARIAEGDPAVFTRLYAEQARAAADAADARRRDGISLGPLDGAMVSIKDLFDVAGETTRAGSILLADAAPA
RADAPVVARLRRAGAVILGKTNMSEFAFSGLGLNPHYGTPGNAADPARIPGGSSSGAGVSVGQGTSDIAIGSDTGGSVRI
PAALNGVVGFKPTASRVPLEGAFPLSYALDSLGPLARTVADCAAADAVMAGEEPAPLEALAVAGLRIGVPRGLLFTETEG
PVAEAFERVLSRLSRAGARIADHPIDDHLAAMDAALPDGSLAAIEAAAIHADWLDSAADRYDPRVHRRIVAGRGATAAGY
IRTMRRRAALIAAFDRYLAPLDVLALPATATTAPLTAPLAADDAAFLAANRLMLRNTAFGNFFDLTGLSLPMPDLPRPAG
LMLLARHGQDRRLLAIGAGVEAALAE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=221, Percent_Identity=35.7466063348416, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI222831590, Length=475, Percent_Identity=26.7368421052632, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI166795287, Length=157, Percent_Identity=36.9426751592357, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=196, Percent_Identity=37.2448979591837, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=314, Percent_Identity=28.9808917197452, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=159, Percent_Identity=37.7358490566038, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=160, Percent_Identity=37.5, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=163, Percent_Identity=35.5828220858896, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=150, Percent_Identity=36, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=150, Percent_Identity=36, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=398, Percent_Identity=30.4020100502513, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=29.03981264637, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648113, Length=263, Percent_Identity=36.8821292775665, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550774, Length=258, Percent_Identity=28.6821705426357, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648435, Length=258, Percent_Identity=28.6821705426357, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648437, Length=258, Percent_Identity=28.6821705426357, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652985, Length=234, Percent_Identity=32.9059829059829, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922090, Length=234, Percent_Identity=32.9059829059829, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652981, Length=234, Percent_Identity=32.9059829059829, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652983, Length=234, Percent_Identity=32.9059829059829, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648441, Length=215, Percent_Identity=31.6279069767442, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648439, Length=215, Percent_Identity=31.6279069767442, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24644968, Length=420, Percent_Identity=24.047619047619, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161078093, Length=215, Percent_Identity=35.3488372093023, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356731, Length=409, Percent_Identity=23.960880195599, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 43908; Mature: 43777

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRETVEERLARIAEGDPAVFTRLYAEQARAAADAADARRRDGISLGPLDGAMVSIKDL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHH
FDVAGETTRAGSILLADAAPARADAPVVARLRRAGAVILGKTNMSEFAFSGLGLNPHYGT
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCC
PGNAADPARIPGGSSSGAGVSVGQGTSDIAIGSDTGGSVRIPAALNGVVGFKPTASRVPL
CCCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCEECCHHHCCCCCCCCCCCCCCC
EGAFPLSYALDSLGPLARTVADCAAADAVMAGEEPAPLEALAVAGLRIGVPRGLLFTETE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCC
GPVAEAFERVLSRLSRAGARIADHPIDDHLAAMDAALPDGSLAAIEAAAIHADWLDSAAD
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RYDPRVHRRIVAGRGATAAGYIRTMRRRAALIAAFDRYLAPLDVLALPATATTAPLTAPL
HCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCC
AADDAAFLAANRLMLRNTAFGNFFDLTGLSLPMPDLPRPAGLMLLARHGQDRRLLAIGAG
CCCCHHHHHHHHHHEECCCCCCCEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECC
VEAALAE
CHHHHCC
>Mature Secondary Structure 
SVRETVEERLARIAEGDPAVFTRLYAEQARAAADAADARRRDGISLGPLDGAMVSIKDL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHH
FDVAGETTRAGSILLADAAPARADAPVVARLRRAGAVILGKTNMSEFAFSGLGLNPHYGT
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCC
PGNAADPARIPGGSSSGAGVSVGQGTSDIAIGSDTGGSVRIPAALNGVVGFKPTASRVPL
CCCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCEECCHHHCCCCCCCCCCCCCCC
EGAFPLSYALDSLGPLARTVADCAAADAVMAGEEPAPLEALAVAGLRIGVPRGLLFTETE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCC
GPVAEAFERVLSRLSRAGARIADHPIDDHLAAMDAALPDGSLAAIEAAAIHADWLDSAAD
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RYDPRVHRRIVAGRGATAAGYIRTMRRRAALIAAFDRYLAPLDVLALPATATTAPLTAPL
HCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCC
AADDAAFLAANRLMLRNTAFGNFFDLTGLSLPMPDLPRPAGLMLLARHGQDRRLLAIGAG
CCCCHHHHHHHHHHEECCCCCCCEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECC
VEAALAE
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA