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The map label for this gene is ubiF [C]

Identifier: 170748078

GI number: 170748078

Start: 1770752

End: 1771981

Strand: Reverse

Name: ubiF [C]

Synonym: Mrad2831_1660

Alternate gene names: 170748078

Gene position: 1771981-1770752 (Counterclockwise)

Preceding gene: 170748079

Following gene: 170748075

Centisome position: 29.15

GC content: 78.29

Gene sequence:

>1230_bases
ATGTCCGAGGGAACCAGGGTCGAAGAAGCCGGCGCGCCGTTCGCGGTCGCGGTGGTCGGAGCCGGGGCAGCCGGGCTCGC
CGCCGCGCTGGCGATCGCGCGCGACGGGATCCCCACGGCGCTGGTCGGGCGCCACGCCCCAGTCGCGGACGGGCGGACCG
TCGCGCTCCTCGACGGGTCGGTGCGCTTCCTGGAGGCGCTCGGCGCCTGGGAGGCGGTGGCGCCGCACGCGAGCCCGCTC
TGCACGCTGCAGATCGTCGACGACACCGGCAGCCTGTTCCGGCCGCCGCCGGCGCAGTTCCGCGCCGCCGAGATCGGCCT
CGACGCCTTCGGCTGGAACGTCGAGAGCGCCCGCCTGGTGGAGAGCCTGCGCGCCCGCGCCCGGGCGCAGGACAACCTCA
CCCTGGTCGAGGCGGACGCCGCCGGCGCCGTGCCCGGCGAGACGGCGGCGCGCGTCGCGCTGGCGGACGGGAACGCCGTC
GAGGCCCGGCTCGTGGTGGGAGCCGACGGGGCGCGCTCGCCGCTGCGGGCCGCCAGCGGCGTCCGCGTGCGCGACTGGAC
CTACCCGCAGAGCGCCATCACCACGATCCTGGCCCACGCGCGCCCGCACCGCGACGTCTCCACGGAGTTCCACACCCGGT
CGGGACCCTTCACCCTGGTGCCGCTGCCGGGCGGCCACCGGTCCAGCCTCGTCTGGGTGACCGGGGAGGGGGCGGCCCGG
CGGCTCGCCGCCCTCGACGACGCGGCGCTGGGACAGGCGGTGGAGCGACAGGCCCGGGCGATGCTCGGCGCCATGCGGGT
CGATGGCCCCCGGGGTCTCGTGCCGATGCGCGGCCTCGCGGTGGCGACCCCGGTAGCCCCGCGGCTCGCGCTGATCGGGG
AGGCGGCCCACGTCTTCCCGCCGATCGGCGCGCAGGGGCTCAATCTCGGCCTGCGCGACGCCGCGACCCTGCGCGACGCG
GTGCTGGCCGCCGCCCGCGACCGCCGCGATCCCGGGTCGCGCGCCGTGCTCGACGGCTACGCGCGGGCGCGCCGCGTCGA
CACGGCGGCGCGCACCGCCGCGGTCGATCTCCTCAACCGCAGCCTGCTCAGCGACCTGCTGCCGGTGGACGCGCTGCGCG
GCCTCGGCCTGCTGGCGATGACGCAGCTCGGACCGCTGCGGCGCCTGGTGATGCGGGAGGGCGTGCTGCCCCAGATCGGC
GCGCCGGAGCTGATGCGCGCGGCGCGCTAG

Upstream 100 bases:

>100_bases
CGGACGCGACGCGACGGCCCTGTCCCGGGCCTACGGCGAGCGTGGTCGGGCGAGCGTGGTCGGGTCGGCACGGGAACGAG
CGGAGATCGTGCGGGGCAGC

Downstream 100 bases:

>100_bases
GACCGCTCCCGATCGTCTTGCGGCCGCCTGGACGAGGGGCATCTCCGGCCGAGGCCGCCGCGCCCTGCCCCCTCTCCCGA
CGGGAGACGGAACCCGCGCC

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSEGTRVEEAGAPFAVAVVGAGAAGLAAALAIARDGIPTALVGRHAPVADGRTVALLDGSVRFLEALGAWEAVAPHASPL
CTLQIVDDTGSLFRPPPAQFRAAEIGLDAFGWNVESARLVESLRARARAQDNLTLVEADAAGAVPGETAARVALADGNAV
EARLVVGADGARSPLRAASGVRVRDWTYPQSAITTILAHARPHRDVSTEFHTRSGPFTLVPLPGGHRSSLVWVTGEGAAR
RLAALDDAALGQAVERQARAMLGAMRVDGPRGLVPMRGLAVATPVAPRLALIGEAAHVFPPIGAQGLNLGLRDAATLRDA
VLAAARDRRDPGSRAVLDGYARARRVDTAARTAAVDLLNRSLLSDLLPVDALRGLGLLAMTQLGPLRRLVMREGVLPQIG
APELMRAAR

Sequences:

>Translated_409_residues
MSEGTRVEEAGAPFAVAVVGAGAAGLAAALAIARDGIPTALVGRHAPVADGRTVALLDGSVRFLEALGAWEAVAPHASPL
CTLQIVDDTGSLFRPPPAQFRAAEIGLDAFGWNVESARLVESLRARARAQDNLTLVEADAAGAVPGETAARVALADGNAV
EARLVVGADGARSPLRAASGVRVRDWTYPQSAITTILAHARPHRDVSTEFHTRSGPFTLVPLPGGHRSSLVWVTGEGAAR
RLAALDDAALGQAVERQARAMLGAMRVDGPRGLVPMRGLAVATPVAPRLALIGEAAHVFPPIGAQGLNLGLRDAATLRDA
VLAAARDRRDPGSRAVLDGYARARRVDTAARTAAVDLLNRSLLSDLLPVDALRGLGLLAMTQLGPLRRLVMREGVLPQIG
APELMRAAR
>Mature_408_residues
SEGTRVEEAGAPFAVAVVGAGAAGLAAALAIARDGIPTALVGRHAPVADGRTVALLDGSVRFLEALGAWEAVAPHASPLC
TLQIVDDTGSLFRPPPAQFRAAEIGLDAFGWNVESARLVESLRARARAQDNLTLVEADAAGAVPGETAARVALADGNAVE
ARLVVGADGARSPLRAASGVRVRDWTYPQSAITTILAHARPHRDVSTEFHTRSGPFTLVPLPGGHRSSLVWVTGEGAARR
LAALDDAALGQAVERQARAMLGAMRVDGPRGLVPMRGLAVATPVAPRLALIGEAAHVFPPIGAQGLNLGLRDAATLRDAV
LAAARDRRDPGSRAVLDGYARARRVDTAARTAAVDLLNRSLLSDLLPVDALRGLGLLAMTQLGPLRRLVMREGVLPQIGA
PELMRAAR

Specific function: Oxygenase That Introduces The Hydroxyl Group At Carbon Five Of 2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol Resulting In The Formation Of 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=399, Percent_Identity=28.0701754385965, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI32967307, Length=275, Percent_Identity=32.3636363636364, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786883, Length=396, Percent_Identity=30.050505050505, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789273, Length=371, Percent_Identity=28.8409703504043, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789274, Length=384, Percent_Identity=28.90625, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25146631, Length=439, Percent_Identity=25.0569476082005, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19920760, Length=382, Percent_Identity=25.130890052356, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42450; Mature: 42319

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGTRVEEAGAPFAVAVVGAGAAGLAAALAIARDGIPTALVGRHAPVADGRTVALLDGS
CCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCC
VRFLEALGAWEAVAPHASPLCTLQIVDDTGSLFRPPPAQFRAAEIGLDAFGWNVESARLV
HHHHHHHCCHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHHCCCCCHHHHHH
ESLRARARAQDNLTLVEADAAGAVPGETAARVALADGNAVEARLVVGADGARSPLRAASG
HHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHCC
VRVRDWTYPQSAITTILAHARPHRDVSTEFHTRSGPFTLVPLPGGHRSSLVWVTGEGAAR
CEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHH
RLAALDDAALGQAVERQARAMLGAMRVDGPRGLVPMRGLAVATPVAPRLALIGEAAHVFP
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEECCCCHHHHHHCCHHHHCC
PIGAQGLNLGLRDAATLRDAVLAAARDRRDPGSRAVLDGYARARRVDTAARTAAVDLLNR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSDLLPVDALRGLGLLAMTQLGPLRRLVMREGVLPQIGAPELMRAAR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
SEGTRVEEAGAPFAVAVVGAGAAGLAAALAIARDGIPTALVGRHAPVADGRTVALLDGS
CCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCC
VRFLEALGAWEAVAPHASPLCTLQIVDDTGSLFRPPPAQFRAAEIGLDAFGWNVESARLV
HHHHHHHCCHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHHCCCCCHHHHHH
ESLRARARAQDNLTLVEADAAGAVPGETAARVALADGNAVEARLVVGADGARSPLRAASG
HHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHCC
VRVRDWTYPQSAITTILAHARPHRDVSTEFHTRSGPFTLVPLPGGHRSSLVWVTGEGAAR
CEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHH
RLAALDDAALGQAVERQARAMLGAMRVDGPRGLVPMRGLAVATPVAPRLALIGEAAHVFP
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEECCCCHHHHHHCCHHHHCC
PIGAQGLNLGLRDAATLRDAVLAAARDRRDPGSRAVLDGYARARRVDTAARTAAVDLLNR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSDLLPVDALRGLGLLAMTQLGPLRRLVMREGVLPQIGAPELMRAAR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]