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The map label for this gene is pncA [H]

Identifier: 170748061

GI number: 170748061

Start: 1752385

End: 1753011

Strand: Reverse

Name: pncA [H]

Synonym: Mrad2831_1643

Alternate gene names: 170748061

Gene position: 1753011-1752385 (Counterclockwise)

Preceding gene: 170748062

Following gene: 170748060

Centisome position: 28.84

GC content: 74.48

Gene sequence:

>627_bases
ATGACGCCCGGCAGCGCCGACCTGCTCCTCGTCGTCGACGTGCAGGTCGATTTCCTGCCGGGCGGGGCGCTCCCGGTCCC
GGAAGGCGACGCGGTGGTCGGCCCGATCAACGACCTCCAGCACCGCTTCCGGCACGTGGTGCTGATGCAGGACTGGCATC
CGGCCGATCACGTCTCCTTCGCCGAGACCCATCCGGGGCGGGCCCCGTTCGAGACCGTGGCGCGGCCCTACGGGCCGCAG
GTGCTGTGGCCGCGCCACTGCGTCCAGGGCAGCCCGGGCGCCGCCTTCGCGCCGGGTCTCGCCACCGACCGGGCGAGCCT
CGTGGTGCGCAAGGGGCTCGATCCCCGGGTCGACAGCTACTCGGCCTTCCTGGAGGCCGACCGGTCGACCCGCACCGGGC
TCGCCGGGGCGCTCAGGGAGCGCGGCATCACCCGCGTCGCCGTGTGCGGCCTCGCCACCGATTTCTGCGTCGCCTGGAGC
GCGCTCGACGCCAGCGACGCGGGTTTCGAGGTCCTCGTGGTGGAGGATGCGGTGCGCGGGATCGACGTGGACGGGTCGCT
CGCGCAGGCCTGGGCGCGCATGGAGGCGGCGGGGGTGCGCCGGATCCGGTCGGCGCAGATCGGCTGA

Upstream 100 bases:

>100_bases
ACGCCACCTACCCGCTGGCCGAGGCGCAGCAGGTCCACCGCGACCTCGCGGGCCGGGAGACCACCGGCTCGATCGTGATG
CTGCCCTGAGGCAGGCGACC

Downstream 100 bases:

>100_bases
GCGGGCTGACCGGCCCCGGGGGCGGGAGGCGAACCGCCGCACGCCGCGCGGGAGACCGGCCCCGCGCCGATGCGTTGGCC
CGGCATCGGCGTCCGTCGGG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTPGSADLLLVVDVQVDFLPGGALPVPEGDAVVGPINDLQHRFRHVVLMQDWHPADHVSFAETHPGRAPFETVARPYGPQ
VLWPRHCVQGSPGAAFAPGLATDRASLVVRKGLDPRVDSYSAFLEADRSTRTGLAGALRERGITRVAVCGLATDFCVAWS
ALDASDAGFEVLVVEDAVRGIDVDGSLAQAWARMEAAGVRRIRSAQIG

Sequences:

>Translated_208_residues
MTPGSADLLLVVDVQVDFLPGGALPVPEGDAVVGPINDLQHRFRHVVLMQDWHPADHVSFAETHPGRAPFETVARPYGPQ
VLWPRHCVQGSPGAAFAPGLATDRASLVVRKGLDPRVDSYSAFLEADRSTRTGLAGALRERGITRVAVCGLATDFCVAWS
ALDASDAGFEVLVVEDAVRGIDVDGSLAQAWARMEAAGVRRIRSAQIG
>Mature_207_residues
TPGSADLLLVVDVQVDFLPGGALPVPEGDAVVGPINDLQHRFRHVVLMQDWHPADHVSFAETHPGRAPFETVARPYGPQV
LWPRHCVQGSPGAAFAPGLATDRASLVVRKGLDPRVDSYSAFLEADRSTRTGLAGALRERGITRVAVCGLATDFCVAWSA
LDASDAGFEVLVVEDAVRGIDVDGSLAQAWARMEAAGVRRIRSAQIG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=195, Percent_Identity=45.1282051282051, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71999339, Length=237, Percent_Identity=33.7552742616034, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193206735, Length=201, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=201, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=30.5699481865285, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648247, Length=199, Percent_Identity=38.1909547738693, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22142; Mature: 22011

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGSADLLLVVDVQVDFLPGGALPVPEGDAVVGPINDLQHRFRHVVLMQDWHPADHVSF
CCCCCCCEEEEEEEEEEEECCCCCCCCCCCEEECCHHHHHHHHHEEEEEECCCCCCCCCC
AETHPGRAPFETVARPYGPQVLWPRHCVQGSPGAAFAPGLATDRASLVVRKGLDPRVDSY
CCCCCCCCCHHHHHCCCCCCEECHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
SAFLEADRSTRTGLAGALRERGITRVAVCGLATDFCVAWSALDASDAGFEVLVVEDAVRG
HHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
IDVDGSLAQAWARMEAAGVRRIRSAQIG
CCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPGSADLLLVVDVQVDFLPGGALPVPEGDAVVGPINDLQHRFRHVVLMQDWHPADHVSF
CCCCCCEEEEEEEEEEEECCCCCCCCCCCEEECCHHHHHHHHHEEEEEECCCCCCCCCC
AETHPGRAPFETVARPYGPQVLWPRHCVQGSPGAAFAPGLATDRASLVVRKGLDPRVDSY
CCCCCCCCCHHHHHCCCCCCEECHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
SAFLEADRSTRTGLAGALRERGITRVAVCGLATDFCVAWSALDASDAGFEVLVVEDAVRG
HHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
IDVDGSLAQAWARMEAAGVRRIRSAQIG
CCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]