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The map label for this gene is purD [H]

Identifier: 170747941

GI number: 170747941

Start: 1602602

End: 1603876

Strand: Reverse

Name: purD [H]

Synonym: Mrad2831_1523

Alternate gene names: 170747941

Gene position: 1603876-1602602 (Counterclockwise)

Preceding gene: 170747942

Following gene: 170747938

Centisome position: 26.39

GC content: 74.67

Gene sequence:

>1275_bases
ATGAACATCCTGCTGATCGGCTCCGGCGGCCGCGAGCACGCCCTGGCCTGGCGACTGGCCCAGAGCCCGCTCTGCACCCG
CCTGTTCACGGCGCCGGGCAATCCCGGCACGGCCCGCCACGGGACCAACCGACCGGACCTCAAGGTCACGGACCACGCGG
CCGTGGCCGCCTTCTGCGCGGCGGAGTCGATCGGCCTCGTGGTGGTCGGGCCCGAGGCGCCCCTCGTCGCCGGCCTGGTG
GACGACCTGAAGGCCGCCGGGATCCGCGCCTTCGGGCCGACCCGCGACGCCGCCCGGCTCGAGGGCTCGAAGGGCTTCAC
CAAGGATCTCTGCGCCGCCTGCAACATCCCCACCGCCGCCTTCGCGCGCTTCACCGAACTGGAGCCGGCGCTGGCCTATG
TCCGCCAGCAGGGGGCCCCGATCGTCGTGAAGGCCGACGGCCTCGCCGCCGGCAAGGGCGTCACCGTGGCCGAAACCGTC
GACCAGGCCGAGGACGCGCTGACCGCCCTCTTCGCCGAGCCCGGCGCCGAGGTGGTGGTCGAGGAGTGCCTGTTCGGCGA
GGAGGCGAGCTTCTTCGCCCTGTGCGACGGCACGCGGGCGATCCCCCTCGGCACCGCCCAGGACCACAAGCGGGTCTTCG
ACGGCGACCGCGGTCCCAATACCGGCGGCATGGGCGCCTATTCCCCGGCCCGGGTGGTCACCCCGGAGATCGAGGCCCGG
GTCATGGCCGAGATCATCGCGCCGACGCTCGCCGGCATGCGGGCCCGCGGCTGCCCGTTCACCGGCATCCTGTACGCCGG
CCTGATGCTCACCGCCGACGGCCCCAAGCTGATCGAGTACAACACCCGCTTCGGCGACCCCGAGGCGCAGGTGCTGATGC
CGCGCCTCGCCTCCGACCTCGTGCCGGCCCTGCTCTCGGCCTGCGACGGCGACCTCTCCGGCGTGGGCCTGGAATTCGAT
CCCCACCGGGCGGCCCTCACCGTGGTGATGGCGGCGGCCGGCTACCCGGGCGCCGTGGTCCGGGGCTCGGTGATCCGGGG
CGTCGACGCCGCCGAGGGCGACGGGGTCCACGTGTTCCAGGCCGGCACCCGCGCCGAGGACGGGCAGTTGCTCGCCGACG
GCGGCCGGGTCCTGGCGGTGACGGCCCTCGGCGACAGCGTCACCGACGCCAAGGCCCGCGCCTACGCGGCGGTGGCCCGG
ATCGACTGGCCGGAGGGTTTCTGCCGGCGCGACATCGGGTTCCGCGCCGTGGCGCGGGAGGCGGGAGAGGGCTGA

Upstream 100 bases:

>100_bases
CCGAGCCGCCGGGATATCCGCGCAGAGCGGGGAAAGCCCGAAAAAAACCTTTTCCCGCGGGCACGCTTCCCCCAGAAGCT
GGGCCCATGGTGGCGTGCGC

Downstream 100 bases:

>100_bases
GCGGCGCCCGTGTTCGACGCCGCGGCGGGCCGTGCGCCCCCTGCCCCGGACGCCCGGGTCGCCGCGGAGGCTGATCCGGG
ACCCGAGCCCGCGCGGTGCC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNILLIGSGGREHALAWRLAQSPLCTRLFTAPGNPGTARHGTNRPDLKVTDHAAVAAFCAAESIGLVVVGPEAPLVAGLV
DDLKAAGIRAFGPTRDAARLEGSKGFTKDLCAACNIPTAAFARFTELEPALAYVRQQGAPIVVKADGLAAGKGVTVAETV
DQAEDALTALFAEPGAEVVVEECLFGEEASFFALCDGTRAIPLGTAQDHKRVFDGDRGPNTGGMGAYSPARVVTPEIEAR
VMAEIIAPTLAGMRARGCPFTGILYAGLMLTADGPKLIEYNTRFGDPEAQVLMPRLASDLVPALLSACDGDLSGVGLEFD
PHRAALTVVMAAAGYPGAVVRGSVIRGVDAAEGDGVHVFQAGTRAEDGQLLADGGRVLAVTALGDSVTDAKARAYAAVAR
IDWPEGFCRRDIGFRAVAREAGEG

Sequences:

>Translated_424_residues
MNILLIGSGGREHALAWRLAQSPLCTRLFTAPGNPGTARHGTNRPDLKVTDHAAVAAFCAAESIGLVVVGPEAPLVAGLV
DDLKAAGIRAFGPTRDAARLEGSKGFTKDLCAACNIPTAAFARFTELEPALAYVRQQGAPIVVKADGLAAGKGVTVAETV
DQAEDALTALFAEPGAEVVVEECLFGEEASFFALCDGTRAIPLGTAQDHKRVFDGDRGPNTGGMGAYSPARVVTPEIEAR
VMAEIIAPTLAGMRARGCPFTGILYAGLMLTADGPKLIEYNTRFGDPEAQVLMPRLASDLVPALLSACDGDLSGVGLEFD
PHRAALTVVMAAAGYPGAVVRGSVIRGVDAAEGDGVHVFQAGTRAEDGQLLADGGRVLAVTALGDSVTDAKARAYAAVAR
IDWPEGFCRRDIGFRAVAREAGEG
>Mature_424_residues
MNILLIGSGGREHALAWRLAQSPLCTRLFTAPGNPGTARHGTNRPDLKVTDHAAVAAFCAAESIGLVVVGPEAPLVAGLV
DDLKAAGIRAFGPTRDAARLEGSKGFTKDLCAACNIPTAAFARFTELEPALAYVRQQGAPIVVKADGLAAGKGVTVAETV
DQAEDALTALFAEPGAEVVVEECLFGEEASFFALCDGTRAIPLGTAQDHKRVFDGDRGPNTGGMGAYSPARVVTPEIEAR
VMAEIIAPTLAGMRARGCPFTGILYAGLMLTADGPKLIEYNTRFGDPEAQVLMPRLASDLVPALLSACDGDLSGVGLEFD
PHRAALTVVMAAAGYPGAVVRGSVIRGVDAAEGDGVHVFQAGTRAEDGQLLADGGRVLAVTALGDSVTDAKARAYAAVAR
IDWPEGFCRRDIGFRAVAREAGEG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=46.0992907801418, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=46.0992907801418, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=46.0992907801418, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=46.0992907801418, Blast_Score=366, Evalue=1e-101,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=55.3738317757009, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=40.3301886792453, Blast_Score=277, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=40, Blast_Score=318, Evalue=7e-88,
Organism=Drosophila melanogaster, GI62471619, Length=412, Percent_Identity=40.7766990291262, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24582400, Length=412, Percent_Identity=40.7766990291262, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43819; Mature: 43819

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLIGSGGREHALAWRLAQSPLCTRLFTAPGNPGTARHGTNRPDLKVTDHAAVAAFCA
CEEEEEECCCCCHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHH
AESIGLVVVGPEAPLVAGLVDDLKAAGIRAFGPTRDAARLEGSKGFTKDLCAACNIPTAA
HHHCCEEEECCCCCCHHHHHHHHHHCCEEECCCCCHHHHCCCCCCCHHHHHHHCCCCHHH
FARFTELEPALAYVRQQGAPIVVKADGLAAGKGVTVAETVDQAEDALTALFAEPGAEVVV
HHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EECLFGEEASFFALCDGTRAIPLGTAQDHKRVFDGDRGPNTGGMGAYSPARVVTPEIEAR
HHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHH
VMAEIIAPTLAGMRARGCPFTGILYAGLMLTADGPKLIEYNTRFGDPEAQVLMPRLASDL
HHHHHHHHHHHCCCCCCCCHHHHHHHCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHH
VPALLSACDGDLSGVGLEFDPHRAALTVVMAAAGYPGAVVRGSVIRGVDAAEGDGVHVFQ
HHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCCHHHCCHHHCCCCCCCCCCEEEEE
AGTRAEDGQLLADGGRVLAVTALGDSVTDAKARAYAAVARIDWPEGFCRRDIGFRAVARE
CCCCCCCCCEEECCCEEEEEEECCCCCHHHHHHHHEEEEEECCCCHHHHHCCCHHHHHHH
AGEG
CCCC
>Mature Secondary Structure
MNILLIGSGGREHALAWRLAQSPLCTRLFTAPGNPGTARHGTNRPDLKVTDHAAVAAFCA
CEEEEEECCCCCHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHH
AESIGLVVVGPEAPLVAGLVDDLKAAGIRAFGPTRDAARLEGSKGFTKDLCAACNIPTAA
HHHCCEEEECCCCCCHHHHHHHHHHCCEEECCCCCHHHHCCCCCCCHHHHHHHCCCCHHH
FARFTELEPALAYVRQQGAPIVVKADGLAAGKGVTVAETVDQAEDALTALFAEPGAEVVV
HHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EECLFGEEASFFALCDGTRAIPLGTAQDHKRVFDGDRGPNTGGMGAYSPARVVTPEIEAR
HHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHH
VMAEIIAPTLAGMRARGCPFTGILYAGLMLTADGPKLIEYNTRFGDPEAQVLMPRLASDL
HHHHHHHHHHHCCCCCCCCHHHHHHHCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHH
VPALLSACDGDLSGVGLEFDPHRAALTVVMAAAGYPGAVVRGSVIRGVDAAEGDGVHVFQ
HHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCCHHHCCHHHCCCCCCCCCCEEEEE
AGTRAEDGQLLADGGRVLAVTALGDSVTDAKARAYAAVARIDWPEGFCRRDIGFRAVARE
CCCCCCCCCEEECCCEEEEEEECCCCCHHHHHHHHEEEEEECCCCHHHHHCCCHHHHHHH
AGEG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]