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The map label for this gene is alkK [H]

Identifier: 170747920

GI number: 170747920

Start: 1571625

End: 1573262

Strand: Reverse

Name: alkK [H]

Synonym: Mrad2831_1502

Alternate gene names: 170747920

Gene position: 1573262-1571625 (Counterclockwise)

Preceding gene: 170747921

Following gene: 170747918

Centisome position: 25.89

GC content: 72.71

Gene sequence:

>1638_bases
ATGGCCCGCGACACGATCTACGACCGCGACCTCGACCGCGTCCCGGCCAACCACCAGCCGCTGACGCCCCTGCTCTACCT
GGACCGGGCCGCGCGGGTGTTCCCCGACCACGTCGCGGTGGTCCACGGCCCCCTGCGGCGCTCCTACCGGGAGGTCTACG
CCCGGGCGCGCCGCCTCGCCGCGGCGCTGGCGGCGCGCGGGATCGGCCGCGGCGACACCGTGGCGGCGCTGCTCGCCAAC
ACCCCCGAGATGATCGAGTGCCACTACGGCGTGCCGATGACCGGGGCGGTGCTCAATACCCTCAACACCCGCCTCGACGC
CGACGCGATCCGCTTCTGCCTGCAGCACGGCGAGGCCGCGGTGCTGATCACCGACCGCGAGTTCTCCCGGACGGCCGCCG
CGGCCCTGGAGGGGCTCGCCACCAAGCCCTTCGTGATCGACGTCGACGATCCCGAATATGACGGCCCGGGCGCCCGCCTC
GGCGCGACCGACTACGAGGCCTTCCTGGCCGGGGGCGACCCGGAGCACGACTGGCGTCTGCCGGACGACGAGTGGGACGC
GATCACCCTGAACTACACGTCGGGCACCACCGGCGACCCGAAGGGCGTGGTCTACCACCATCGCGGGGCGGCGCTGCTGG
CGCTCGGCAACGTCATCACGGGCGGGCTCGGCCAGCACCCGGTCTATCTCTGGACCCTGCCGATGTTCCACTGCAACGGC
TGGTGCTTCCCCTGGACCCTGTCGATCGTGGCCGGGACCCATGTCTGCCTGCGTCAGGTGCGGGCGGAGGCGATGTACCG
GCTGATGGCCGAGCACGGGGTCACGCATCTCTGCGGCGCGCCGATCGTCATGCAGATGCTGATCAACGCCCCCGACGCCG
AGCGCCGCGACCTGCCCCGGCGCGTCGCCTTCTTCACCGCCGCGGCGCCGCCGCCGGAGGCGGTGCTGGCCGGCATGTCG
GAGGCCGGGTTCGACGTGACCCACCTGTACGGCCTGACCGAGAGCTACGGGCCGGCGGTCGTCAACGCCTGGCACGCCGA
CTGGGACGCCCTGGCGGCCGACCAGCGGGCGGCCAAGAAGGCCCGCCAGGGGGTGCGCTACCCGGTGCTCGAGGCCCTCG
ACGTCCGCGACCCGGAGACGCTGGAGCCGGTGCCCGCCGACGGGGCGACGATCGGCGAGGTGATGATGCGCGGCAACGTG
GTGATGCGCGGCTACCTGAAGAACCCGGCCGCCACGCGGGCCGCCTTCGCGGGCGGCTGGTTCCGCACCGGCGACCTCGG
GGTGAAGCACCCGGACGGCTACGTGCAGCTCAAGGACCGCTCGAAGGACATCATCATCTCGGGCGGCGAGAACATCTCGT
CGATCGAGGTCGAGGACGCCCTGTTCAAGCACCCGGCGGTGGCCGCGGCCGCGGTGGTGGCGCGGCCCGACGCGAAATGG
GGCGAGACCCCGTGCGCCTTCGTCGAGCTCAAGGCCGGCGCGGCCCCCACGGCCGACGAGCTGATCCAGTGGTGCCGGGG
GCGGCTGGCCTCCTACAAGCTCCCCCGCCACGTCATCTTCGGCGAATTGCCCAAGACCTCCACCGGCAAGGTGCAGAAGT
TCATCCTGCGGGAGCGGGCCCGGCAGGACGGGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCCCCCGGGTCGACGGCGCGGAAGCCCCGCCGGACCGATTGATCGCGCCCGTTCCGGCAAGCTACTGTCCCGGCAAC
ACATTGCCGGGAGGACGGGG

Downstream 100 bases:

>100_bases
TCGGCGCCGGGCCGGGATCGGGCCCCGACGCCCGGCCCCGGTGTCGCGGGCCGGTCAGGCCTGCGGCGGATCCCGTCGGT
CGGCGCGGACCGGCACCGGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MARDTIYDRDLDRVPANHQPLTPLLYLDRAARVFPDHVAVVHGPLRRSYREVYARARRLAAALAARGIGRGDTVAALLAN
TPEMIECHYGVPMTGAVLNTLNTRLDADAIRFCLQHGEAAVLITDREFSRTAAAALEGLATKPFVIDVDDPEYDGPGARL
GATDYEAFLAGGDPEHDWRLPDDEWDAITLNYTSGTTGDPKGVVYHHRGAALLALGNVITGGLGQHPVYLWTLPMFHCNG
WCFPWTLSIVAGTHVCLRQVRAEAMYRLMAEHGVTHLCGAPIVMQMLINAPDAERRDLPRRVAFFTAAAPPPEAVLAGMS
EAGFDVTHLYGLTESYGPAVVNAWHADWDALAADQRAAKKARQGVRYPVLEALDVRDPETLEPVPADGATIGEVMMRGNV
VMRGYLKNPAATRAAFAGGWFRTGDLGVKHPDGYVQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVARPDAKW
GETPCAFVELKAGAAPTADELIQWCRGRLASYKLPRHVIFGELPKTSTGKVQKFILRERARQDGA

Sequences:

>Translated_545_residues
MARDTIYDRDLDRVPANHQPLTPLLYLDRAARVFPDHVAVVHGPLRRSYREVYARARRLAAALAARGIGRGDTVAALLAN
TPEMIECHYGVPMTGAVLNTLNTRLDADAIRFCLQHGEAAVLITDREFSRTAAAALEGLATKPFVIDVDDPEYDGPGARL
GATDYEAFLAGGDPEHDWRLPDDEWDAITLNYTSGTTGDPKGVVYHHRGAALLALGNVITGGLGQHPVYLWTLPMFHCNG
WCFPWTLSIVAGTHVCLRQVRAEAMYRLMAEHGVTHLCGAPIVMQMLINAPDAERRDLPRRVAFFTAAAPPPEAVLAGMS
EAGFDVTHLYGLTESYGPAVVNAWHADWDALAADQRAAKKARQGVRYPVLEALDVRDPETLEPVPADGATIGEVMMRGNV
VMRGYLKNPAATRAAFAGGWFRTGDLGVKHPDGYVQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVARPDAKW
GETPCAFVELKAGAAPTADELIQWCRGRLASYKLPRHVIFGELPKTSTGKVQKFILRERARQDGA
>Mature_544_residues
ARDTIYDRDLDRVPANHQPLTPLLYLDRAARVFPDHVAVVHGPLRRSYREVYARARRLAAALAARGIGRGDTVAALLANT
PEMIECHYGVPMTGAVLNTLNTRLDADAIRFCLQHGEAAVLITDREFSRTAAAALEGLATKPFVIDVDDPEYDGPGARLG
ATDYEAFLAGGDPEHDWRLPDDEWDAITLNYTSGTTGDPKGVVYHHRGAALLALGNVITGGLGQHPVYLWTLPMFHCNGW
CFPWTLSIVAGTHVCLRQVRAEAMYRLMAEHGVTHLCGAPIVMQMLINAPDAERRDLPRRVAFFTAAAPPPEAVLAGMSE
AGFDVTHLYGLTESYGPAVVNAWHADWDALAADQRAAKKARQGVRYPVLEALDVRDPETLEPVPADGATIGEVMMRGNVV
MRGYLKNPAATRAAFAGGWFRTGDLGVKHPDGYVQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVARPDAKWG
ETPCAFVELKAGAAPTADELIQWCRGRLASYKLPRHVIFGELPKTSTGKVQKFILRERARQDGA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=26.7148014440433, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI187761345, Length=554, Percent_Identity=25.812274368231, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI187761343, Length=554, Percent_Identity=25.812274368231, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=26.171875, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=26.171875, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI58082049, Length=513, Percent_Identity=26.5107212475634, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=26.8199233716475, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI42544132, Length=564, Percent_Identity=25.531914893617, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI38505220, Length=518, Percent_Identity=27.027027027027, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI115511026, Length=521, Percent_Identity=27.447216890595, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI28416953, Length=124, Percent_Identity=33.8709677419355, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI8923896, Length=129, Percent_Identity=34.8837209302326, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI253970489, Length=129, Percent_Identity=34.8837209302326, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=30.550284629981, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI221142682, Length=498, Percent_Identity=27.3092369477912, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1788107, Length=559, Percent_Identity=25.7602862254025, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=27.9850746268657, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1790505, Length=555, Percent_Identity=23.4234234234234, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788595, Length=471, Percent_Identity=24.416135881104, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=531, Percent_Identity=28.060263653484, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71994690, Length=531, Percent_Identity=28.060263653484, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71994703, Length=531, Percent_Identity=28.060263653484, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=27.3809523809524, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=533, Percent_Identity=26.266416510319, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=24.3713733075435, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=375, Percent_Identity=26.9333333333333, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=516, Percent_Identity=24.2248062015504, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=538, Percent_Identity=23.7918215613383, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=530, Percent_Identity=23.9622641509434, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=523, Percent_Identity=21.6061185468451, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=156, Percent_Identity=37.8205128205128, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=542, Percent_Identity=24.9077490774908, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=502, Percent_Identity=24.5019920318725, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=536, Percent_Identity=24.6268656716418, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323182, Length=141, Percent_Identity=31.9148936170213, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581924, Length=564, Percent_Identity=26.9503546099291, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI18859661, Length=512, Percent_Identity=26.953125, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21355181, Length=359, Percent_Identity=27.8551532033426, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24583465, Length=457, Percent_Identity=25.8205689277899, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583463, Length=457, Percent_Identity=25.8205689277899, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281364798, Length=457, Percent_Identity=25.8205689277899, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648253, Length=501, Percent_Identity=23.9520958083832, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648255, Length=501, Percent_Identity=23.75249500998, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45550496, Length=459, Percent_Identity=25.2723311546841, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45552801, Length=459, Percent_Identity=25.2723311546841, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922652, Length=513, Percent_Identity=24.3664717348928, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648260, Length=508, Percent_Identity=24.6062992125984, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356441, Length=515, Percent_Identity=22.1359223300971, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356947, Length=548, Percent_Identity=22.6277372262774, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=440, Percent_Identity=25.9090909090909, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 59314; Mature: 59183

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDTIYDRDLDRVPANHQPLTPLLYLDRAARVFPDHVAVVHGPLRRSYREVYARARRLA
CCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHH
AALAARGIGRGDTVAALLANTPEMIECHYGVPMTGAVLNTLNTRLDADAIRFCLQHGEAA
HHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEE
VLITDREFSRTAAAALEGLATKPFVIDVDDPEYDGPGARLGATDYEAFLAGGDPEHDWRL
EEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCC
PDDEWDAITLNYTSGTTGDPKGVVYHHRGAALLALGNVITGGLGQHPVYLWTLPMFHCNG
CCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEHHHHHCCCCCCCCEEEEEEHHHHCCC
WCFPWTLSIVAGTHVCLRQVRAEAMYRLMAEHGVTHLCGAPIVMQMLINAPDAERRDLPR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCHHHCCCH
RVAFFTAAAPPPEAVLAGMSEAGFDVTHLYGLTESYGPAVVNAWHADWDALAADQRAAKK
HEEEEECCCCCHHHHHHCCHHCCCCHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
ARQGVRYPVLEALDVRDPETLEPVPADGATIGEVMMRGNVVMRGYLKNPAATRAAFAGGW
HHCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHCCCC
FRTGDLGVKHPDGYVQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVARPDAKW
EEECCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHHHHCCCCCCC
GETPCAFVELKAGAAPTADELIQWCRGRLASYKLPRHVIFGELPKTSTGKVQKFILRERA
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCHHHHHHHHHHHH
RQDGA
HCCCC
>Mature Secondary Structure 
ARDTIYDRDLDRVPANHQPLTPLLYLDRAARVFPDHVAVVHGPLRRSYREVYARARRLA
CCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHH
AALAARGIGRGDTVAALLANTPEMIECHYGVPMTGAVLNTLNTRLDADAIRFCLQHGEAA
HHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEE
VLITDREFSRTAAAALEGLATKPFVIDVDDPEYDGPGARLGATDYEAFLAGGDPEHDWRL
EEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCC
PDDEWDAITLNYTSGTTGDPKGVVYHHRGAALLALGNVITGGLGQHPVYLWTLPMFHCNG
CCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEHHHHHCCCCCCCCEEEEEEHHHHCCC
WCFPWTLSIVAGTHVCLRQVRAEAMYRLMAEHGVTHLCGAPIVMQMLINAPDAERRDLPR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCHHHCCCH
RVAFFTAAAPPPEAVLAGMSEAGFDVTHLYGLTESYGPAVVNAWHADWDALAADQRAAKK
HEEEEECCCCCHHHHHHCCHHCCCCHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
ARQGVRYPVLEALDVRDPETLEPVPADGATIGEVMMRGNVVMRGYLKNPAATRAAFAGGW
HHCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHCCCC
FRTGDLGVKHPDGYVQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVARPDAKW
EEECCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHHHHCCCCCCC
GETPCAFVELKAGAAPTADELIQWCRGRLASYKLPRHVIFGELPKTSTGKVQKFILRERA
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCHHHHHHHHHHHH
RQDGA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]