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The map label for this gene is czcB [H]

Identifier: 170747821

GI number: 170747821

Start: 1475457

End: 1476836

Strand: Reverse

Name: czcB [H]

Synonym: Mrad2831_1403

Alternate gene names: 170747821

Gene position: 1476836-1475457 (Counterclockwise)

Preceding gene: 170747822

Following gene: 170747820

Centisome position: 24.3

GC content: 75.29

Gene sequence:

>1380_bases
ATGCGCATCGTCCTGTCCGTGGCCGCCGCCGCGGCACTTCTGGCCGCCGGCTTCGCCCTCGGCTCCGCCGTTCCCGCCGT
CCCCACCCTGCCCGACCGTCTCCGGCAGACCCTCGCGGCCGCCGGACTCGCCCGCCCTCCGGCCGACGATCCCGATCATC
GCGCCGCGGCCGCCGCACCGGGCCGGCCGGAGGCCGCGCCCGATCCGCACGCGGGCGACGATCACGCCGACCACGGCCGC
GCGCACGACCACGCGCACGACCACGCGCACGGCGCCGACGAGGCCCACGAGCGGGGCACGATCCGGATGCGGCCCGACCA
AGTCGTCGGCCAGGACATCGTCACGCGGACGGTCGGGGGCGGCACGCTGGCGCGCCACCTCCTCGTCCCCGGCACGATCA
CGCCCGACACCGACCGGATCGCCCGGGTCCCGGCCCGGGTGGTCGGGACCGTGGCCGAGATGCGCAAGCGCCTCGGCGAC
GCCGTGCAGACGAACGAGGTCGTCGCCATCCTCGACAGCCGCGAGGTCGCCGACGCCAAGAGCGAGTACCTCACCGCCCA
GGTCCAGGCCGAGCTGCAGACGATCAACTTCGAGCGCCAGCAGAAGCTGCTCGCCTCGCGCAGCGCCTCGGAGGCGGCCT
TCGAGACCGCCCGCGCCGCCTATCTGGAGAACCGGCTCCGGGTCGACCTCGCCCGCCAGAAGCTGTCGGCGCTCGGCCTC
GACGCCGCCGAGGTGGCGGCCGCGCAGAAGCGCGACGAGGCCACGCCGAACCAGTCGACCCTGCGCCGCTACCCCCTGCG
GGCGCCGCTCGCCGGGCGCATCGTCGAGCGCAAGGTCGATGTCGGAACGGCGGTCGGCAAGGAGGGCGATCCGGCCGACC
TCTACACGGTGGCCGACCTCTCCACGGTCTGGATCGAGCTGTCGGTGCCGACCCTCGACCTCGCCCAGGTGCGGGAGGGC
GCCCGCGTGACGGTCACGCCCGGCCGGACGGGCGAGGCGGAGCGCCGGGCCGAGGGCCGGGTGATCTTCGTCAGCCCCTT
CCTCAACCCCGACACGCGCTCGGCCCGCGTGGTGGTCGCCCTGCCGAATCCCGACCTCGCGTGGCGGCCCGGCACCTTCG
TGACCGCCGAAGTCGAGATCGCGCAGGACGCGGTCCCGGTCCGGATCCCCAAGGCGGCCGTCCAGACCATCGCGGGCCGG
AGCGCCGTCTTCGTGCGCACGGCCGAGGGGTTCGAGAAGCGGCCGGTCACCCTCGGCCGGTCCGACGACGAGGCCTACGA
GGTCACCGCCGGGCTCGAGCCCGGCGCCGAGATCGCGGTCGGCAACGCGTTCCTGCTGAAGGCCGAGCTCGGCAAGGCCG
ACGCCGGCCACGACCACTGA

Upstream 100 bases:

>100_bases
TTCCGAGCAGGCTCCGGGTTCGCCCTCACGTGTCACCTGAACGGGACCTCCCGGGACCGCCGCGCGCGGCACCCCGCCGT
CCCGGCCGAGCAGCTTCCCC

Downstream 100 bases:

>100_bases
GGAGACGGACGATGATCAGCCGCATCCTCGCCGTCTCGGTCCGCCAGCGCTGGCTGGTGCTGCTCCTCGTGCTGCTGGCC
TCGGGGTTCGGCGCCGCCGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRIVLSVAAAAALLAAGFALGSAVPAVPTLPDRLRQTLAAAGLARPPADDPDHRAAAAAPGRPEAAPDPHAGDDHADHGR
AHDHAHDHAHGADEAHERGTIRMRPDQVVGQDIVTRTVGGGTLARHLLVPGTITPDTDRIARVPARVVGTVAEMRKRLGD
AVQTNEVVAILDSREVADAKSEYLTAQVQAELQTINFERQQKLLASRSASEAAFETARAAYLENRLRVDLARQKLSALGL
DAAEVAAAQKRDEATPNQSTLRRYPLRAPLAGRIVERKVDVGTAVGKEGDPADLYTVADLSTVWIELSVPTLDLAQVREG
ARVTVTPGRTGEAERRAEGRVIFVSPFLNPDTRSARVVVALPNPDLAWRPGTFVTAEVEIAQDAVPVRIPKAAVQTIAGR
SAVFVRTAEGFEKRPVTLGRSDDEAYEVTAGLEPGAEIAVGNAFLLKAELGKADAGHDH

Sequences:

>Translated_459_residues
MRIVLSVAAAAALLAAGFALGSAVPAVPTLPDRLRQTLAAAGLARPPADDPDHRAAAAAPGRPEAAPDPHAGDDHADHGR
AHDHAHDHAHGADEAHERGTIRMRPDQVVGQDIVTRTVGGGTLARHLLVPGTITPDTDRIARVPARVVGTVAEMRKRLGD
AVQTNEVVAILDSREVADAKSEYLTAQVQAELQTINFERQQKLLASRSASEAAFETARAAYLENRLRVDLARQKLSALGL
DAAEVAAAQKRDEATPNQSTLRRYPLRAPLAGRIVERKVDVGTAVGKEGDPADLYTVADLSTVWIELSVPTLDLAQVREG
ARVTVTPGRTGEAERRAEGRVIFVSPFLNPDTRSARVVVALPNPDLAWRPGTFVTAEVEIAQDAVPVRIPKAAVQTIAGR
SAVFVRTAEGFEKRPVTLGRSDDEAYEVTAGLEPGAEIAVGNAFLLKAELGKADAGHDH
>Mature_459_residues
MRIVLSVAAAAALLAAGFALGSAVPAVPTLPDRLRQTLAAAGLARPPADDPDHRAAAAAPGRPEAAPDPHAGDDHADHGR
AHDHAHDHAHGADEAHERGTIRMRPDQVVGQDIVTRTVGGGTLARHLLVPGTITPDTDRIARVPARVVGTVAEMRKRLGD
AVQTNEVVAILDSREVADAKSEYLTAQVQAELQTINFERQQKLLASRSASEAAFETARAAYLENRLRVDLARQKLSALGL
DAAEVAAAQKRDEATPNQSTLRRYPLRAPLAGRIVERKVDVGTAVGKEGDPADLYTVADLSTVWIELSVPTLDLAQVREG
ARVTVTPGRTGEAERRAEGRVIFVSPFLNPDTRSARVVVALPNPDLAWRPGTFVTAEVEIAQDAVPVRIPKAAVQTIAGR
SAVFVRTAEGFEKRPVTLGRSDDEAYEVTAGLEPGAEIAVGNAFLLKAELGKADAGHDH

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=350, Percent_Identity=24.5714285714286, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48707; Mature: 48707

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVLSVAAAAALLAAGFALGSAVPAVPTLPDRLRQTLAAAGLARPPADDPDHRAAAAAP
CEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GRPEAAPDPHAGDDHADHGRAHDHAHDHAHGADEAHERGTIRMRPDQVVGQDIVTRTVGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCHHHHHHHHHHHCCCC
GTLARHLLVPGTITPDTDRIARVPARVVGTVAEMRKRLGDAVQTNEVVAILDSREVADAK
HHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHH
SEYLTAQVQAELQTINFERQQKLLASRSASEAAFETARAAYLENRLRVDLARQKLSALGL
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DAAEVAAAQKRDEATPNQSTLRRYPLRAPLAGRIVERKVDVGTAVGKEGDPADLYTVADL
CHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEECC
STVWIELSVPTLDLAQVREGARVTVTPGRTGEAERRAEGRVIFVSPFLNPDTRSARVVVA
EEEEEEEECCCCCHHHHCCCCEEEECCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEE
LPNPDLAWRPGTFVTAEVEIAQDAVPVRIPKAAVQTIAGRSAVFVRTAEGFEKRPVTLGR
ECCCCCCCCCCCEEEEEEEECCCCCCEECCHHHHHHHCCCCEEEEEECCCCCCCCEECCC
SDDEAYEVTAGLEPGAEIAVGNAFLLKAELGKADAGHDH
CCCCCEEEEECCCCCCEEEECCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MRIVLSVAAAAALLAAGFALGSAVPAVPTLPDRLRQTLAAAGLARPPADDPDHRAAAAAP
CEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GRPEAAPDPHAGDDHADHGRAHDHAHDHAHGADEAHERGTIRMRPDQVVGQDIVTRTVGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCHHHHHHHHHHHCCCC
GTLARHLLVPGTITPDTDRIARVPARVVGTVAEMRKRLGDAVQTNEVVAILDSREVADAK
HHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHH
SEYLTAQVQAELQTINFERQQKLLASRSASEAAFETARAAYLENRLRVDLARQKLSALGL
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DAAEVAAAQKRDEATPNQSTLRRYPLRAPLAGRIVERKVDVGTAVGKEGDPADLYTVADL
CHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEECC
STVWIELSVPTLDLAQVREGARVTVTPGRTGEAERRAEGRVIFVSPFLNPDTRSARVVVA
EEEEEEEECCCCCHHHHCCCCEEEECCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEE
LPNPDLAWRPGTFVTAEVEIAQDAVPVRIPKAAVQTIAGRSAVFVRTAEGFEKRPVTLGR
ECCCCCCCCCCCEEEEEEEECCCCCCEECCHHHHHHHCCCCEEEEEECCCCCCCCEECCC
SDDEAYEVTAGLEPGAEIAVGNAFLLKAELGKADAGHDH
CCCCCEEEEECCCCCCEEEECCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]