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The map label for this gene is purR [H]

Identifier: 170747728

GI number: 170747728

Start: 1364792

End: 1365847

Strand: Reverse

Name: purR [H]

Synonym: Mrad2831_1307

Alternate gene names: 170747728

Gene position: 1365847-1364792 (Counterclockwise)

Preceding gene: 170747730

Following gene: 170747724

Centisome position: 22.47

GC content: 75.95

Gene sequence:

>1056_bases
ATGGGCCAGACGACGATCAAGGACGTCGCCCGACGGGCCGGCGTGAGCTTCCAGACGGTCTCGCTGGTCCTGAACCACCC
GCAGAAGGTCGCCCGGACCACGCGCGAGACCATCGAGACGGCCATGCGCGACCTGGACTTCGTGCCCAGCCTCGCCGCCC
GGTCCCTGCGCAAGAAGCCGAGCCGCAGCATCGCCTGCGTGTTCGCCCCCGCGCCGGAGCCCGCCGCCGACGGGTCCCGC
CCGGGGGAGGAGACCGACCACGGCCGCGTCCTCCAGACCTTCGGCACCGTCACCGACCAGCAGGGCTACACCCTGGTCCA
CCGCTGCCGCCGCGGCGACGACCCGGACGGCCCGGGCCGGATCGCCAGCCTCGTCAGCGAGGCCCGGGTCGACGGCCTCG
TGGTCTTCGCCGAGGGCCGGGACGACCCGCTGATCGGCCTGCTCCAGGCCAAGCGGTGCCCGTTCGTGGTGTTCGGCCTG
GACGTGCCCGGCACGCACTATGCGGCGCGGGCCGAGCGCGACGCGATCCACGGGGCCGTTCGCCACCTGATCGCGGCGGG
GAGCCGGTCGATCTGCCTGCTCACGGGCGACGCGCGCCGGCCGGCCGCCGACACCGAGCGGTTCCGCGGCTACAGGGACG
CCCTCGCGGAGGCCGGCCTGCCCCTGCGCGACGCCCTCGTGGTCCCCTGCGACGGGACCGTCGCGGGCGGCTACCGGGCC
GCGCGGGCGCTGCTGGAGACCGGGCCCGGCGCCCGCCCGGACGCCGTCGTGGCCGCCAGCGACGGGGTGGCGCTCGGCGC
GCTGCGGGCGCTGCACGAGGGCGGCGTGCGGGTCCCCGCGGAGATCCGCGTCGTCGGCTTCGACAACCTCGCCCAGGGGG
CCTACGCGGTCCCGAGCCTGACCTCGGTCGATGTGCCGGTGCTCGACATGGTCCGGTTCGCCTTCGCGACGCTGGTCGAG
CTGATCGAGGGGACGCGGGCGCCGGACGACCTCGCCCAGAAGCTGTTTCCCACGCGCCTCGTCGTGCGCGAGTCGAGCGC
GGCGGCGCCGTCCTAG

Upstream 100 bases:

>100_bases
TTATCTCCGATCGATACAGATCTAGATTGCCGCACCGAACGAGGCGGCGCCGCGCGCCCGCGCCCGGCCGCGACCGTTCG
GACCGGCTCGCGAGGGGACG

Downstream 100 bases:

>100_bases
CGGAGTCTGTGGCCGGTTCTCACTCCGCCGCGAGGACGCCCGCGCCGGATGCGCCGCGCCCGAACTGCGCCGCGTGCAGG
CGGCTGTAGGGGCCGCCGGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MGQTTIKDVARRAGVSFQTVSLVLNHPQKVARTTRETIETAMRDLDFVPSLAARSLRKKPSRSIACVFAPAPEPAADGSR
PGEETDHGRVLQTFGTVTDQQGYTLVHRCRRGDDPDGPGRIASLVSEARVDGLVVFAEGRDDPLIGLLQAKRCPFVVFGL
DVPGTHYAARAERDAIHGAVRHLIAAGSRSICLLTGDARRPAADTERFRGYRDALAEAGLPLRDALVVPCDGTVAGGYRA
ARALLETGPGARPDAVVAASDGVALGALRALHEGGVRVPAEIRVVGFDNLAQGAYAVPSLTSVDVPVLDMVRFAFATLVE
LIEGTRAPDDLAQKLFPTRLVVRESSAAAPS

Sequences:

>Translated_351_residues
MGQTTIKDVARRAGVSFQTVSLVLNHPQKVARTTRETIETAMRDLDFVPSLAARSLRKKPSRSIACVFAPAPEPAADGSR
PGEETDHGRVLQTFGTVTDQQGYTLVHRCRRGDDPDGPGRIASLVSEARVDGLVVFAEGRDDPLIGLLQAKRCPFVVFGL
DVPGTHYAARAERDAIHGAVRHLIAAGSRSICLLTGDARRPAADTERFRGYRDALAEAGLPLRDALVVPCDGTVAGGYRA
ARALLETGPGARPDAVVAASDGVALGALRALHEGGVRVPAEIRVVGFDNLAQGAYAVPSLTSVDVPVLDMVRFAFATLVE
LIEGTRAPDDLAQKLFPTRLVVRESSAAAPS
>Mature_350_residues
GQTTIKDVARRAGVSFQTVSLVLNHPQKVARTTRETIETAMRDLDFVPSLAARSLRKKPSRSIACVFAPAPEPAADGSRP
GEETDHGRVLQTFGTVTDQQGYTLVHRCRRGDDPDGPGRIASLVSEARVDGLVVFAEGRDDPLIGLLQAKRCPFVVFGLD
VPGTHYAARAERDAIHGAVRHLIAAGSRSICLLTGDARRPAADTERFRGYRDALAEAGLPLRDALVVPCDGTVAGGYRAA
RALLETGPGARPDAVVAASDGVALGALRALHEGGVRVPAEIRVVGFDNLAQGAYAVPSLTSVDVPVLDMVRFAFATLVEL
IEGTRAPDDLAQKLFPTRLVVRESSAAAPS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=351, Percent_Identity=31.0541310541311, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787948, Length=351, Percent_Identity=31.6239316239316, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790194, Length=348, Percent_Identity=29.0229885057471, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789202, Length=349, Percent_Identity=30.0859598853868, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790369, Length=354, Percent_Identity=25.9887005649718, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786540, Length=360, Percent_Identity=28.8888888888889, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788474, Length=348, Percent_Identity=28.735632183908, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=25.4491017964072, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=26.0736196319018, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994940, Length=316, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787906, Length=346, Percent_Identity=26.3005780346821, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37180; Mature: 37048

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTTIKDVARRAGVSFQTVSLVLNHPQKVARTTRETIETAMRDLDFVPSLAARSLRKKP
CCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SRSIACVFAPAPEPAADGSRPGEETDHGRVLQTFGTVTDQQGYTLVHRCRRGDDPDGPGR
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHH
IASLVSEARVDGLVVFAEGRDDPLIGLLQAKRCPFVVFGLDVPGTHYAARAERDAIHGAV
HHHHHHHHCCCEEEEEECCCCCCEEHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
RHLIAAGSRSICLLTGDARRPAADTERFRGYRDALAEAGLPLRDALVVPCDGTVAGGYRA
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCHHHH
ARALLETGPGARPDAVVAASDGVALGALRALHEGGVRVPAEIRVVGFDNLAQGAYAVPSL
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCC
TSVDVPVLDMVRFAFATLVELIEGTRAPDDLAQKLFPTRLVVRESSAAAPS
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEEEECCCCCCCC
>Mature Secondary Structure 
GQTTIKDVARRAGVSFQTVSLVLNHPQKVARTTRETIETAMRDLDFVPSLAARSLRKKP
CCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SRSIACVFAPAPEPAADGSRPGEETDHGRVLQTFGTVTDQQGYTLVHRCRRGDDPDGPGR
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHH
IASLVSEARVDGLVVFAEGRDDPLIGLLQAKRCPFVVFGLDVPGTHYAARAERDAIHGAV
HHHHHHHHCCCEEEEEECCCCCCEEHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
RHLIAAGSRSICLLTGDARRPAADTERFRGYRDALAEAGLPLRDALVVPCDGTVAGGYRA
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCHHHH
ARALLETGPGARPDAVVAASDGVALGALRALHEGGVRVPAEIRVVGFDNLAQGAYAVPSL
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCC
TSVDVPVLDMVRFAFATLVELIEGTRAPDDLAQKLFPTRLVVRESSAAAPS
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA