Click here to switch to the map view.

The map label for this gene is czcB [H]

Identifier: 170747664

GI number: 170747664

Start: 1304469

End: 1305824

Strand: Reverse

Name: czcB [H]

Synonym: Mrad2831_1239

Alternate gene names: 170747664

Gene position: 1305824-1304469 (Counterclockwise)

Preceding gene: 170747665

Following gene: 170747663

Centisome position: 21.49

GC content: 75.88

Gene sequence:

>1356_bases
ATGGCGCTGACCACCGAGACGGATGCCGGCGACCGTGACGTCGACTGCCGCGACGCCGGGCGCGCCGACGCGGGCGCAGC
GTCCAGGCGGGAGCCGGACGCGGCCGGCGCCGCGGAGGCCCGCGACGCCGGCCGCCGCGGCGGCGCGCGGGTCTGGCTGG
CCCTCGTCCTCGTCGTCCTCGCGGCAGCGGGCTGGGTCTATCGGGACGCGCTCCGCGAGCGGCTCGGCCTGGCGCCCGCG
GCGCCGCCCGTGACCGGCGCGGCCGACGCCGCGCCGGCGGACCCCGACCGCGTCACCCTCGACGCGGCGGGACAGAAGCG
GATCGGCCTCGCCTTCGGGACGGCCGAGACCCGCCGGATCGTGCTGCCTTTGCGCGTCCCCGGCACCGTCGCCTTCGACG
AGCGGCGGGTGACCCATCTCAAGCCGCGCACGCAAGGACGGGTGCTGAGCCTCGCGGTCCAGCCCGGCGACCGGGTCCAG
GCGGGCGAGACCCTCGCGACCCTCGACGCCGCCGGCCTCCTCGACGCGCGCAACGGCTATCTCGCCGCGCAGGCCTCCCT
CGGCGAGGCGCGGGCCTCCGAGGCGGTGGCGGCGCTGCAGCTCAAGCGCGGGCAGGAGCAGCTCAAGTTCGGCGGCGTCG
CGCAGGCCGAGGTGGAGCGGCGGCAGGTCGATCTCGCCAAGGCGCAGGCGGCGGTGAAATCGGCCCAGGCCAACGCCGAG
ATGTACCGGGCGCAGTACCAGCGCCTCGCCCCGACCGAGGGCGGCGCCCCCGGGACCAGCGCCGTCGTGACCCCGATACA
GGGCGTCGTGACCAGCGTCGGCGTGACCCTGGGCGAGGTCGTCGACACCACCCGCGACGCCGTCACGGTCGCCGACCCGA
CCCGCGTCCTCGTCCTGGCCAACCTCTACGGACCCGATCTCGGGCGGGTGAAGGCCGGCGACACCGCCTCCGTGGAGAGT
CCGATCCCGGGCCAGGCTCCCCTCGACGGCCGCGTCCGCTCGGTGAACGCCGCCCTCGATCCGAGCACCAACACGGCGCC
CGCCCGGATCGAGCTCGACAACCCGGGCGATCTGCTGCGCGCCAACATGTTCGTCTCCGTGACGATCGCGGCCGATCTCG
GCCGCGACGGGATCACCGTCCCGGCGGCCGCGGTGCAGCAGACCGAGCAGGGGCCGATCGCCTTCGTGCGCGTGGCCGAG
GACCGGTTCGAGCGGCGCGCCCTCAAACTCGGGATCCAGCGCTCCGACTGGGTCGAGGTGCAGAGCGGCGTCGCGGCCGG
GGAGAGCGTGGCGACGCAGGGCAGCTTCGGGCTCAAGGCGATCCTGCTGCGCAGCCTGCTCGGCTCGACGGATTGA

Upstream 100 bases:

>100_bases
CTCCGGCCGGGCCTCGACGCGTGAGCGGGCGCGGCACGAGTGCGCGCGCGCGGCGGCCCGGGGGAGCCGCCGGCGGCAGG
AAATCCCGGCGGGCGTGCCC

Downstream 100 bases:

>100_bases
GGCTCCCGATGCGGTCGTGGTTCGCCCTCCTGGTCCGCCGCCGGTTCCTGGTCCTGATGATCGCGCTCGCCGCGGCGGCG
GGCGGCGTGGCCAACCTCGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MALTTETDAGDRDVDCRDAGRADAGAASRREPDAAGAAEARDAGRRGGARVWLALVLVVLAAAGWVYRDALRERLGLAPA
APPVTGAADAAPADPDRVTLDAAGQKRIGLAFGTAETRRIVLPLRVPGTVAFDERRVTHLKPRTQGRVLSLAVQPGDRVQ
AGETLATLDAAGLLDARNGYLAAQASLGEARASEAVAALQLKRGQEQLKFGGVAQAEVERRQVDLAKAQAAVKSAQANAE
MYRAQYQRLAPTEGGAPGTSAVVTPIQGVVTSVGVTLGEVVDTTRDAVTVADPTRVLVLANLYGPDLGRVKAGDTASVES
PIPGQAPLDGRVRSVNAALDPSTNTAPARIELDNPGDLLRANMFVSVTIAADLGRDGITVPAAAVQQTEQGPIAFVRVAE
DRFERRALKLGIQRSDWVEVQSGVAAGESVATQGSFGLKAILLRSLLGSTD

Sequences:

>Translated_451_residues
MALTTETDAGDRDVDCRDAGRADAGAASRREPDAAGAAEARDAGRRGGARVWLALVLVVLAAAGWVYRDALRERLGLAPA
APPVTGAADAAPADPDRVTLDAAGQKRIGLAFGTAETRRIVLPLRVPGTVAFDERRVTHLKPRTQGRVLSLAVQPGDRVQ
AGETLATLDAAGLLDARNGYLAAQASLGEARASEAVAALQLKRGQEQLKFGGVAQAEVERRQVDLAKAQAAVKSAQANAE
MYRAQYQRLAPTEGGAPGTSAVVTPIQGVVTSVGVTLGEVVDTTRDAVTVADPTRVLVLANLYGPDLGRVKAGDTASVES
PIPGQAPLDGRVRSVNAALDPSTNTAPARIELDNPGDLLRANMFVSVTIAADLGRDGITVPAAAVQQTEQGPIAFVRVAE
DRFERRALKLGIQRSDWVEVQSGVAAGESVATQGSFGLKAILLRSLLGSTD
>Mature_450_residues
ALTTETDAGDRDVDCRDAGRADAGAASRREPDAAGAAEARDAGRRGGARVWLALVLVVLAAAGWVYRDALRERLGLAPAA
PPVTGAADAAPADPDRVTLDAAGQKRIGLAFGTAETRRIVLPLRVPGTVAFDERRVTHLKPRTQGRVLSLAVQPGDRVQA
GETLATLDAAGLLDARNGYLAAQASLGEARASEAVAALQLKRGQEQLKFGGVAQAEVERRQVDLAKAQAAVKSAQANAEM
YRAQYQRLAPTEGGAPGTSAVVTPIQGVVTSVGVTLGEVVDTTRDAVTVADPTRVLVLANLYGPDLGRVKAGDTASVESP
IPGQAPLDGRVRSVNAALDPSTNTAPARIELDNPGDLLRANMFVSVTIAADLGRDGITVPAAAVQQTEQGPIAFVRVAED
RFERRALKLGIQRSDWVEVQSGVAAGESVATQGSFGLKAILLRSLLGSTD

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=310, Percent_Identity=23.5483870967742, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46810; Mature: 46679

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTTETDAGDRDVDCRDAGRADAGAASRREPDAAGAAEARDAGRRGGARVWLALVLVVL
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
AAAGWVYRDALRERLGLAPAAPPVTGAADAAPADPDRVTLDAAGQKRIGLAFGTAETRRI
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEE
VLPLRVPGTVAFDERRVTHLKPRTQGRVLSLAVQPGDRVQAGETLATLDAAGLLDARNGY
EEEEECCCEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCEECCCCE
LAAQASLGEARASEAVAALQLKRGQEQLKFGGVAQAEVERRQVDLAKAQAAVKSAQANAE
EEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYRAQYQRLAPTEGGAPGTSAVVTPIQGVVTSVGVTLGEVVDTTRDAVTVADPTRVLVLA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
NLYGPDLGRVKAGDTASVESPIPGQAPLDGRVRSVNAALDPSTNTAPARIELDNPGDLLR
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCEEE
ANMFVSVTIAADLGRDGITVPAAAVQQTEQGPIAFVRVAEDRFERRALKLGIQRSDWVEV
EEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCHHHH
QSGVAAGESVATQGSFGLKAILLRSLLGSTD
HHCCCCCCHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALTTETDAGDRDVDCRDAGRADAGAASRREPDAAGAAEARDAGRRGGARVWLALVLVVL
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
AAAGWVYRDALRERLGLAPAAPPVTGAADAAPADPDRVTLDAAGQKRIGLAFGTAETRRI
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEE
VLPLRVPGTVAFDERRVTHLKPRTQGRVLSLAVQPGDRVQAGETLATLDAAGLLDARNGY
EEEEECCCEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCEECCCCE
LAAQASLGEARASEAVAALQLKRGQEQLKFGGVAQAEVERRQVDLAKAQAAVKSAQANAE
EEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYRAQYQRLAPTEGGAPGTSAVVTPIQGVVTSVGVTLGEVVDTTRDAVTVADPTRVLVLA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
NLYGPDLGRVKAGDTASVESPIPGQAPLDGRVRSVNAALDPSTNTAPARIELDNPGDLLR
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCEEE
ANMFVSVTIAADLGRDGITVPAAAVQQTEQGPIAFVRVAEDRFERRALKLGIQRSDWVEV
EEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCHHHH
QSGVAAGESVATQGSFGLKAILLRSLLGSTD
HHCCCCCCHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]