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The map label for this gene is btuF [H]

Identifier: 170747577

GI number: 170747577

Start: 1205747

End: 1206523

Strand: Reverse

Name: btuF [H]

Synonym: Mrad2831_1145

Alternate gene names: 170747577

Gene position: 1206523-1205747 (Counterclockwise)

Preceding gene: 170747578

Following gene: 170747576

Centisome position: 19.85

GC content: 75.16

Gene sequence:

>777_bases
ATGCGCTGCTTCCCGCCCGAGCGGATCGTCTGCCTCACCGAGGAGACGGTCGAGACCCTGTACCTCCTGGGCGCGGAGGA
CAGGATCGTCGGCGTCTCGGGCTACGCGGTGCGCCCGCCCCGGGTCCGCCGGGAGAAGCCGCGCGTCTCCGCCTTCACCA
GCGCGGACATCCCGAAGATCCTGGCGCTGGCGCCGGACCTCGTCCTGGCCTTCTCGGACCTCCAGGCCGGCATCGTCGCC
GACCTCGCCCGCGCGGGCGTGGCGGTCCACCTGTTCAACCAGCGCGACGTGGCGGGCTGCCTCGCGATGGTGCGCACGCT
CGGCGCCCTCGTGGGCCTGCCGGAGCGGGCCGAGACCCTGGCGGCGGGCTTCGAGGCGCGGCTGGCCGCGGCCGCCGCGG
AGGTGCGGGACCGGCCGCCCCCGCGCGTCTACTTCGAGGAGTGGGACGCGCCGATGATCTCCGGCATCGGCTGGGTCTCG
GACCTGATCGGCCTCGCGGGCGGCCGGGACGTCTTTCCGGAGTTGAGCCGCCAGCCGGCCGCGAGGGACCGGATCGTCAC
CGCCGAGCAGGTGATCGCGGCCGCGCCCGACGTGATCCTCGCCTCGTGGTGCGGCAAGCGGGTCAACGCCGAGCGCATCC
GGTCCCGGCCCGGCTGGGCGGCGATCCCGGCGGTGCGCGACGGGCGCATCCACGAGATCAAGTCGCCGCTGATCCTGCAG
CCCGGCCCGGCCGCCCTCACCGACGGGTTCGACGCGATCCGGGCCTGCCTCGGGTGA

Upstream 100 bases:

>100_bases
CTGGCCAACGTCGCCTCCGCTCTGGTCTGGGCCTTCGTGCTGCTGGCCCCCGGGGCCGGGCTGCTGACGTGGCTCGACCG
ATGACCCGGCGCGGCCGCTG

Downstream 100 bases:

>100_bases
TCGCCGCTGGGCGATGCCGTGGGGCGATGCCGCGCCGCAGGTGACGGACCGGGCGCGACTTGGCAGGATGTCCCGATTCG
GTACAGACGCACCCGCAGAC

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRCFPPERIVCLTEETVETLYLLGAEDRIVGVSGYAVRPPRVRREKPRVSAFTSADIPKILALAPDLVLAFSDLQAGIVA
DLARAGVAVHLFNQRDVAGCLAMVRTLGALVGLPERAETLAAGFEARLAAAAAEVRDRPPPRVYFEEWDAPMISGIGWVS
DLIGLAGGRDVFPELSRQPAARDRIVTAEQVIAAAPDVILASWCGKRVNAERIRSRPGWAAIPAVRDGRIHEIKSPLILQ
PGPAALTDGFDAIRACLG

Sequences:

>Translated_258_residues
MRCFPPERIVCLTEETVETLYLLGAEDRIVGVSGYAVRPPRVRREKPRVSAFTSADIPKILALAPDLVLAFSDLQAGIVA
DLARAGVAVHLFNQRDVAGCLAMVRTLGALVGLPERAETLAAGFEARLAAAAAEVRDRPPPRVYFEEWDAPMISGIGWVS
DLIGLAGGRDVFPELSRQPAARDRIVTAEQVIAAAPDVILASWCGKRVNAERIRSRPGWAAIPAVRDGRIHEIKSPLILQ
PGPAALTDGFDAIRACLG
>Mature_258_residues
MRCFPPERIVCLTEETVETLYLLGAEDRIVGVSGYAVRPPRVRREKPRVSAFTSADIPKILALAPDLVLAFSDLQAGIVA
DLARAGVAVHLFNQRDVAGCLAMVRTLGALVGLPERAETLAAGFEARLAAAAAEVRDRPPPRVYFEEWDAPMISGIGWVS
DLIGLAGGRDVFPELSRQPAARDRIVTAEQVIAAAPDVILASWCGKRVNAERIRSRPGWAAIPAVRDGRIHEIKSPLILQ
PGPAALTDGFDAIRACLG

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=196, Percent_Identity=28.0612244897959, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 27714; Mature: 27714

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCFPPERIVCLTEETVETLYLLGAEDRIVGVSGYAVRPPRVRREKPRVSAFTSADIPKI
CCCCCCCCEEEECHHHHHHHHHCCCCCCEEECCCCEECCCHHHCCCCCCHHCCCCCHHHH
LALAPDLVLAFSDLQAGIVADLARAGVAVHLFNQRDVAGCLAMVRTLGALVGLPERAETL
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHH
AAGFEARLAAAAAEVRDRPPPRVYFEEWDAPMISGIGWVSDLIGLAGGRDVFPELSRQPA
HHHHHHHHHHHHHHHHCCCCCCEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
ARDRIVTAEQVIAAAPDVILASWCGKRVNAERIRSRPGWAAIPAVRDGRIHEIKSPLILQ
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHCCCCCEECCCCCCCCHHHHCCCEEEC
PGPAALTDGFDAIRACLG
CCCCHHHCCHHHHHHHHC
>Mature Secondary Structure
MRCFPPERIVCLTEETVETLYLLGAEDRIVGVSGYAVRPPRVRREKPRVSAFTSADIPKI
CCCCCCCCEEEECHHHHHHHHHCCCCCCEEECCCCEECCCHHHCCCCCCHHCCCCCHHHH
LALAPDLVLAFSDLQAGIVADLARAGVAVHLFNQRDVAGCLAMVRTLGALVGLPERAETL
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHH
AAGFEARLAAAAAEVRDRPPPRVYFEEWDAPMISGIGWVSDLIGLAGGRDVFPELSRQPA
HHHHHHHHHHHHHHHHCCCCCCEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
ARDRIVTAEQVIAAAPDVILASWCGKRVNAERIRSRPGWAAIPAVRDGRIHEIKSPLILQ
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHCCCCCEECCCCCCCCHHHHCCCEEEC
PGPAALTDGFDAIRACLG
CCCCHHHCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA