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The map label for this gene is ligT [C]

Identifier: 170747445

GI number: 170747445

Start: 1069391

End: 1069930

Strand: Reverse

Name: ligT [C]

Synonym: Mrad2831_1013

Alternate gene names: 170747445

Gene position: 1069930-1069391 (Counterclockwise)

Preceding gene: 170747446

Following gene: 170747441

Centisome position: 17.6

GC content: 73.52

Gene sequence:

>540_bases
ATGCCGCGCCTGTTCACCGGGCTCGCCGTGCCGGCGGAGATCGCCGAGGCGCTCAAGGTCTACCAGGGCGGCTTGCCCGG
CGCCCGCTGGATCGAGCCGGGCGACTTCCACGTCACCCTGCGCTTCCTCGGCGACGTGGAGGCGACCGTGGCCGACGACG
TCGTCGAGGCCCTGTCGGAGATGCGGGCGCGCCCGGCCCTGACGGTGACGCTCGACGGACTCGGCATCTTCGGCGGCGAC
AGGCCCCGCGCGCTCTACGCGTCGGTGGTGCCGGAGGCGGGGCTGATGGATCTGCAGGCCGAGCAGGAGCGGCTCGTGCG
CCGCGCCGGGGTCGAGCCGGAACGGCGGAAATTCACCCCGCACGTGACCCTGGCCCGGCTGCGGCGCGACGCCACGCCGG
AGGCGACCGCGATGTACCTGTCGCAGGCGCCGGTCTTCGCGCCGCTGACTTACACGTCGGACCGGGTGACGCTGTTCTCG
GCGCGGGACTCGACCGGGGGCGGGCCGTATGTGGCGGAGGCGGAATTTCCGTTCGCGTGA

Upstream 100 bases:

>100_bases
TGATCCACCCGAACCGGCAGGGCGTGGAGACCATCGCGGCCCGCATGGCGCCCACGGTCGAGGCGTTCCTGGACCGAATC
CGCCAGCAGCCGGTGCGCTG

Downstream 100 bases:

>100_bases
GCGGGCTGCTCCCACCCGCGCCCTCATCCTGAGGTGCCGGAGCGGAGGTCTCGAAGAAGGGTGCGGTCTGGAGCCCCGAA
CCGGCCTACAGCCGCCTCAG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MPRLFTGLAVPAEIAEALKVYQGGLPGARWIEPGDFHVTLRFLGDVEATVADDVVEALSEMRARPALTVTLDGLGIFGGD
RPRALYASVVPEAGLMDLQAEQERLVRRAGVEPERRKFTPHVTLARLRRDATPEATAMYLSQAPVFAPLTYTSDRVTLFS
ARDSTGGGPYVAEAEFPFA

Sequences:

>Translated_179_residues
MPRLFTGLAVPAEIAEALKVYQGGLPGARWIEPGDFHVTLRFLGDVEATVADDVVEALSEMRARPALTVTLDGLGIFGGD
RPRALYASVVPEAGLMDLQAEQERLVRRAGVEPERRKFTPHVTLARLRRDATPEATAMYLSQAPVFAPLTYTSDRVTLFS
ARDSTGGGPYVAEAEFPFA
>Mature_178_residues
PRLFTGLAVPAEIAEALKVYQGGLPGARWIEPGDFHVTLRFLGDVEATVADDVVEALSEMRARPALTVTLDGLGIFGGDR
PRALYASVVPEAGLMDLQAEQERLVRRAGVEPERRKFTPHVTLARLRRDATPEATAMYLSQAPVFAPLTYTSDRVTLFSA
RDSTGGGPYVAEAEFPFA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19431; Mature: 19300

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFTGLAVPAEIAEALKVYQGGLPGARWIEPGDFHVTLRFLGDVEATVADDVVEALSE
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHH
MRARPALTVTLDGLGIFGGDRPRALYASVVPEAGLMDLQAEQERLVRRAGVEPERRKFTP
HHCCCCEEEEECCEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCC
HVTLARLRRDATPEATAMYLSQAPVFAPLTYTSDRVTLFSARDSTGGGPYVAEAEFPFA
CHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
PRLFTGLAVPAEIAEALKVYQGGLPGARWIEPGDFHVTLRFLGDVEATVADDVVEALSE
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHH
MRARPALTVTLDGLGIFGGDRPRALYASVVPEAGLMDLQAEQERLVRRAGVEPERRKFTP
HHCCCCEEEEECCEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCC
HVTLARLRRDATPEATAMYLSQAPVFAPLTYTSDRVTLFSARDSTGGGPYVAEAEFPFA
CHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]