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The map label for this gene is gyrB [H]

Identifier: 170747435

GI number: 170747435

Start: 1058454

End: 1060514

Strand: Reverse

Name: gyrB [H]

Synonym: Mrad2831_1003

Alternate gene names: 170747435

Gene position: 1060514-1058454 (Counterclockwise)

Preceding gene: 170747437

Following gene: 170747432

Centisome position: 17.45

GC content: 70.98

Gene sequence:

>2061_bases
GTGAGCGACCCGGCGCGGGACCTGTTCGGGCCGGGCGGCAAGGGCGCGGCGGCCCGTCCGCCCGAGCCGCGGCGGCGCCT
GGACGCCTCGGCCTCCGTGGCGACGCTCGCGGCCGCCGAGCCCGAGGGCGGCTACACCGCCTCCGACATCGAGGTGCTGG
AGGGGCTGGAGCCCGTCCGGCGCCGGCCCGGCATGTATATCGGCGGCACCGACGAGCGGGCGCTGCACCACCTCTTCGCC
GAGGTGATCGACAACTCGATGGACGAGGCGGTCGCCGGCCATGCCAGCTTCATCGAGGTGGAACTGGAGGAATCCGGCGC
CCTCGTGGTCACCGACAACGGCCGCGGCATCCCGGTCGACCCGCACCCGAAGTTCCCCGGCAAGTCGGCGCTCGAGGTCA
TCATGACCACGCTGCACGCCGGCGGTAAGTTCGACTCGAAGGTCTACGAGACCTCGGGCGGCCTGCACGGCGTCGGCATC
TCGGTGGTGAACGCGCTCTCCGACCTGCTGGAGGTCGAGGTCGCGCGCAACCAGACCCTCTACCGCCAGACCTTCTCGCG
CGGTCACGCGCAGGGCGCGTTGGAGCTGGTCGGCCGGGTGCAGAACCGGCGCGGCACCCGGGTCCGTTTCCACCCGGACG
CCCAGATCTTCGGCTCACTGAAGTTCGACCCGCGCCGCCTGTTCAAGATGGCGCGCTCGAAGGCCTACCTGTTCGGCGGC
GTCGAGATCCGCTGGCGCTGCGCTCCCGCCCTCCTCGAGGGGCTGGCGGACGTGCCGGCCGAGGCGGTCCACCGCTTCCC
CGACGGGCTGCGCGACTATCTGGCCCGGGACATCGACGGCAAGGAGCTCGTCACCGAGGCGATGTTCTCCGGCAAGATCA
CCAAGCCGGGCTCGCACGGGTCGCTCGAATGGGCGGTGGCCTGGATGGTGGCCGAGGACGGGTTCTCGCACTCCTACTGC
AACACCATCCCGACCCCCGAGGGCGGCACCCACGAGGCGGGCCTCCGCGTCGCGCTGCTGCGCGGCCTGCGCGAGCACGC
CGAGCGGGTGAACCAGGCCAAGCGCATGACGGCGGTGACCACCGACGACGTCATGGCGACCTGCGCGTCGATGCTCTCGG
TGTTCATCCGCGAGCCCGAGTTCCAGGGTCAGACCAAGGACAAGCTCGCCACCGTGGAGGCCCAGCGCATCGTCGAGACG
GCGGTGCGCGACGCCTTCGACCACTGGCTCGCCGCCTCCCCGGCCCAGGCCAACAAGCTCCTCGACTGGGTGATCGACCG
GGCCGAGGAGCGCCTGCGCCGCCGCCAGGAGAAGGAGGTCGCCCGCAAGAGCGCGACCCGCAAGCTGCGCCTGCCCGGCA
AGCTCGCCGACTGCTCGGCGGCCGGCACCGCGGGCTCGGAGATCTTCATCGTCGAGGGCGACTCGGCCGGCGGCTCCGCC
AAGCAGGCCCGGGACCGGGCGACCCAGGCGATCCTGCCCCTGCGCGGCAAGATCCTGAACGTCGCCTCGGCCTCGCGCGA
CAAGCTCGGCGCCAACCAGCTGATCTCGGATCTGACCCTGGCGCTCGGCTGCGGCACCGGCACGGCCTTCCGCGAGACGG
ACCTGCGCTACGAGAAGGTGATCATCATGACGGACGCCGACGTGGACGGCGCCCACATCGCCTCGCTGCTGATCACGTTC
TTCTACCGCCAGATGCCCAAGCTGATCGACCGCGGCCACCTCTACCTCGCGATCCCGCCGCTCTACCGACTGCAGCAGGG
CTCGAAGGTGGCCTATGCCCGGGACGACGCGGATCGCGAGCGGATCGTCAAGACGGTGTTCAAGGGCGGCAAGGTCGAGA
TCGGGCGGTTCAAGGGCCTCGGCGAGATGATGCCGGCCCAGCTCAAGGAGACCACCATGGACCCGAAGAAGCGCACGCTC
CTGCGCGTCGCGGTCCTAGACGAGGCCCGGGAATCGACCGGCGACACGGTCGAGCGCCTGATGGGCAACAAGCCCGAGGC
GCGCTTCGCCTTCATCAGCGAGCGCGCGGCCTTCGCGGAGGGCGCCGACCTCGACATCTGA

Upstream 100 bases:

>100_bases
TGCGGGGATGAAGGCGCATGCTCCACGACCCGAGACCCGTCCCAAGACCCGTCCGCGCGATTCCGAGCCGGCCGACGCGC
CGCGCGCGCGGAGGCCGCGC

Downstream 100 bases:

>100_bases
CGCAGTCCCGACGCGGCCCCGCCGCGTCGGGCGACGGCCTCCGGTCGCGGCCTAGCGCGGGGCCGCGATCGCCTTGGCCG
GGTCGAAGGGCTGGGCCGCC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MSDPARDLFGPGGKGAAARPPEPRRRLDASASVATLAAAEPEGGYTASDIEVLEGLEPVRRRPGMYIGGTDERALHHLFA
EVIDNSMDEAVAGHASFIEVELEESGALVVTDNGRGIPVDPHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGI
SVVNALSDLLEVEVARNQTLYRQTFSRGHAQGALELVGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGG
VEIRWRCAPALLEGLADVPAEAVHRFPDGLRDYLARDIDGKELVTEAMFSGKITKPGSHGSLEWAVAWMVAEDGFSHSYC
NTIPTPEGGTHEAGLRVALLRGLREHAERVNQAKRMTAVTTDDVMATCASMLSVFIREPEFQGQTKDKLATVEAQRIVET
AVRDAFDHWLAASPAQANKLLDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTAGSEIFIVEGDSAGGSA
KQARDRATQAILPLRGKILNVASASRDKLGANQLISDLTLALGCGTGTAFRETDLRYEKVIIMTDADVDGAHIASLLITF
FYRQMPKLIDRGHLYLAIPPLYRLQQGSKVAYARDDADRERIVKTVFKGGKVEIGRFKGLGEMMPAQLKETTMDPKKRTL
LRVAVLDEARESTGDTVERLMGNKPEARFAFISERAAFAEGADLDI

Sequences:

>Translated_686_residues
MSDPARDLFGPGGKGAAARPPEPRRRLDASASVATLAAAEPEGGYTASDIEVLEGLEPVRRRPGMYIGGTDERALHHLFA
EVIDNSMDEAVAGHASFIEVELEESGALVVTDNGRGIPVDPHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGI
SVVNALSDLLEVEVARNQTLYRQTFSRGHAQGALELVGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGG
VEIRWRCAPALLEGLADVPAEAVHRFPDGLRDYLARDIDGKELVTEAMFSGKITKPGSHGSLEWAVAWMVAEDGFSHSYC
NTIPTPEGGTHEAGLRVALLRGLREHAERVNQAKRMTAVTTDDVMATCASMLSVFIREPEFQGQTKDKLATVEAQRIVET
AVRDAFDHWLAASPAQANKLLDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTAGSEIFIVEGDSAGGSA
KQARDRATQAILPLRGKILNVASASRDKLGANQLISDLTLALGCGTGTAFRETDLRYEKVIIMTDADVDGAHIASLLITF
FYRQMPKLIDRGHLYLAIPPLYRLQQGSKVAYARDDADRERIVKTVFKGGKVEIGRFKGLGEMMPAQLKETTMDPKKRTL
LRVAVLDEARESTGDTVERLMGNKPEARFAFISERAAFAEGADLDI
>Mature_685_residues
SDPARDLFGPGGKGAAARPPEPRRRLDASASVATLAAAEPEGGYTASDIEVLEGLEPVRRRPGMYIGGTDERALHHLFAE
VIDNSMDEAVAGHASFIEVELEESGALVVTDNGRGIPVDPHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGIS
VVNALSDLLEVEVARNQTLYRQTFSRGHAQGALELVGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGGV
EIRWRCAPALLEGLADVPAEAVHRFPDGLRDYLARDIDGKELVTEAMFSGKITKPGSHGSLEWAVAWMVAEDGFSHSYCN
TIPTPEGGTHEAGLRVALLRGLREHAERVNQAKRMTAVTTDDVMATCASMLSVFIREPEFQGQTKDKLATVEAQRIVETA
VRDAFDHWLAASPAQANKLLDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTAGSEIFIVEGDSAGGSAK
QARDRATQAILPLRGKILNVASASRDKLGANQLISDLTLALGCGTGTAFRETDLRYEKVIIMTDADVDGAHIASLLITFF
YRQMPKLIDRGHLYLAIPPLYRLQQGSKVAYARDDADRERIVKTVFKGGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLL
RVAVLDEARESTGDTVERLMGNKPEARFAFISERAAFAEGADLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=632, Percent_Identity=25.3164556962025, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI19913406, Length=632, Percent_Identity=25.9493670886076, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1789408, Length=643, Percent_Identity=43.8569206842924, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI48994957, Length=560, Percent_Identity=43.2142857142857, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535065, Length=593, Percent_Identity=24.4519392917369, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=576, Percent_Identity=25.5208333333333, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=206, Percent_Identity=27.6699029126214, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=590, Percent_Identity=25.5932203389831, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI17136538, Length=587, Percent_Identity=25.3833049403748, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74918; Mature: 74787

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPARDLFGPGGKGAAARPPEPRRRLDASASVATLAAAEPEGGYTASDIEVLEGLEPVR
CCCCCCHHCCCCCCCCCCCCCCHHHHHCCCCCHHEEEECCCCCCCCHHHHHHHHCCHHHH
RRPGMYIGGTDERALHHLFAEVIDNSMDEAVAGHASFIEVELEESGALVVTDNGRGIPVD
HCCCEEECCCHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCC
PHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDLLEVEVARNQTL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHH
YRQTFSRGHAQGALELVGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGG
HHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCHHHEECCCCCHHHHHHHHHCCEEEEEC
VEIRWRCAPALLEGLADVPAEAVHRFPDGLRDYLARDIDGKELVTEAMFSGKITKPGSHG
EEEEEEEHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
SLEWAVAWMVAEDGFSHSYCNTIPTPEGGTHEAGLRVALLRGLREHAERVNQAKRMTAVT
CEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDDVMATCASMLSVFIREPEFQGQTKDKLATVEAQRIVETAVRDAFDHWLAASPAQANKL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTAGSEIFIVEGDSAGGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
KQARDRATQAILPLRGKILNVASASRDKLGANQLISDLTLALGCGTGTAFRETDLRYEKV
HHHHHHHHHHHHHHCCHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEE
IIMTDADVDGAHIASLLITFFYRQMPKLIDRGHLYLAIPPLYRLQQGSKVAYARDDADRE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCEEEEECCCHHHH
RIVKTVFKGGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLLRVAVLDEARESTGDTVERL
HHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
MGNKPEARFAFISERAAFAEGADLDI
HCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDPARDLFGPGGKGAAARPPEPRRRLDASASVATLAAAEPEGGYTASDIEVLEGLEPVR
CCCCCHHCCCCCCCCCCCCCCHHHHHCCCCCHHEEEECCCCCCCCHHHHHHHHCCHHHH
RRPGMYIGGTDERALHHLFAEVIDNSMDEAVAGHASFIEVELEESGALVVTDNGRGIPVD
HCCCEEECCCHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCC
PHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDLLEVEVARNQTL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHH
YRQTFSRGHAQGALELVGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGG
HHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCHHHEECCCCCHHHHHHHHHCCEEEEEC
VEIRWRCAPALLEGLADVPAEAVHRFPDGLRDYLARDIDGKELVTEAMFSGKITKPGSHG
EEEEEEEHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
SLEWAVAWMVAEDGFSHSYCNTIPTPEGGTHEAGLRVALLRGLREHAERVNQAKRMTAVT
CEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDDVMATCASMLSVFIREPEFQGQTKDKLATVEAQRIVETAVRDAFDHWLAASPAQANKL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTAGSEIFIVEGDSAGGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
KQARDRATQAILPLRGKILNVASASRDKLGANQLISDLTLALGCGTGTAFRETDLRYEKV
HHHHHHHHHHHHHHCCHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEE
IIMTDADVDGAHIASLLITFFYRQMPKLIDRGHLYLAIPPLYRLQQGSKVAYARDDADRE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCEEEEECCCHHHH
RIVKTVFKGGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLLRVAVLDEARESTGDTVERL
HHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
MGNKPEARFAFISERAAFAEGADLDI
HCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]