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The map label for this gene is pleD [H]

Identifier: 170747430

GI number: 170747430

Start: 1053034

End: 1054092

Strand: Reverse

Name: pleD [H]

Synonym: Mrad2831_0998

Alternate gene names: 170747430

Gene position: 1054092-1053034 (Counterclockwise)

Preceding gene: 170747431

Following gene: 170747427

Centisome position: 17.34

GC content: 70.07

Gene sequence:

>1059_bases
ATGAGCCAGGAGACCTATTCGGATCGCGATCGCAGCTTCGCGCTGGCGAAGCGCAGCCACGATCTGATTCGCGACTACGG
CCCCTCCGCGACGCCGCGCGCCTACGCCGTCTGGTACGCCTACGTCTCGGGGGAGCTGCCGCTCCTCGCCGACGCCGTGA
AGCGCCTCACCACCCAGAATGGCTGCCTCACCGAGGGCGACATCGACGACCTGCACGAGACCTATCTCGACGGGCGCCGC
CTCGCGAGCACCACCGACGACGTCAGCCGCGTCGTCCTCAGCGAGATCGCGGCCGTCACCGAGATCCTGGACCTCTCCCT
GGGCTCGACGGCGCAGTACAGCGAGTCCCTGCGCGGGGTCGCGCAGGATCTCTCCCAGGGCGTCCCGAGCCGGGCGCGGT
TCGGCGAGATCGTCGCGACCCTGGCCGCGACGACCCGGGAGGTCGCCGCCAACAACCGGATCCTCGAAGCCCGCATGCGC
GAGACCCGCAGCGAGATCGAGACCCTGCGCGAGAAGCTCGACGCGACCCGGCGCGAGAGCCTGACCGACGCCCTGACGGG
GCTGGCGAACCGCAAGCATTTCGAGGAGACGCTGAAGGCGTCCATGGAGACGGCCCGGGCGCGCACGACGCCCATGAGCC
TGATCATCCTCGACATCGACTTCTTCAAGCGCTTCAACGACCTGTACGGTCATCTCACGGGCGACCAGGTCCTGCGGCTC
GTGGCGATCGTGATGCGCGAGAACGCCGGCAAGCAGGCGCAGCTCGCCCGGTTCGGCGGCGAGGAGTTCGGGATCATCCT
GCCCGGCGTCGACCGGGCGGTGGCCAAGCAGGTGGCCGAGACGGTGCGCACCAGCGTCATGGGGCGCGAGCTCGTCAAGC
GCTCGACCGGCGAGTCGCTCGGCAAGATCACGATCTCCCTGGGTGTCGCGGTGCTGCGCCCGGGCGACACGCCGGCCTCG
CTGATGGAGCGGGCCGACCTGTGCATGTTCGCCGCGAAGCGCGCGGGCCGGAACCGCTTCCTGGACGACGCCGCCGAGTC
GCTGTCGCAGGTGGCCTGA

Upstream 100 bases:

>100_bases
AGACGTCTGTTCGGGCTTAACTGTCCGGCTACCAGGCGGCCGTATATCAAGTCTGGGTGACGGCGCATGCGCGCCGGTCG
CCCCGGACCGCGGGATACGG

Downstream 100 bases:

>100_bases
GGGCGCGGCGCGCCGCCGGCTCGCTGCGCGCCGGGGCGCTCCCGCTCAGGCCTGCGCGGGCAGGCGGTCCGCCGAGACCG
GCGTGATCGCCACGGATTCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSQETYSDRDRSFALAKRSHDLIRDYGPSATPRAYAVWYAYVSGELPLLADAVKRLTTQNGCLTEGDIDDLHETYLDGRR
LASTTDDVSRVVLSEIAAVTEILDLSLGSTAQYSESLRGVAQDLSQGVPSRARFGEIVATLAATTREVAANNRILEARMR
ETRSEIETLREKLDATRRESLTDALTGLANRKHFEETLKASMETARARTTPMSLIILDIDFFKRFNDLYGHLTGDQVLRL
VAIVMRENAGKQAQLARFGGEEFGIILPGVDRAVAKQVAETVRTSVMGRELVKRSTGESLGKITISLGVAVLRPGDTPAS
LMERADLCMFAAKRAGRNRFLDDAAESLSQVA

Sequences:

>Translated_352_residues
MSQETYSDRDRSFALAKRSHDLIRDYGPSATPRAYAVWYAYVSGELPLLADAVKRLTTQNGCLTEGDIDDLHETYLDGRR
LASTTDDVSRVVLSEIAAVTEILDLSLGSTAQYSESLRGVAQDLSQGVPSRARFGEIVATLAATTREVAANNRILEARMR
ETRSEIETLREKLDATRRESLTDALTGLANRKHFEETLKASMETARARTTPMSLIILDIDFFKRFNDLYGHLTGDQVLRL
VAIVMRENAGKQAQLARFGGEEFGIILPGVDRAVAKQVAETVRTSVMGRELVKRSTGESLGKITISLGVAVLRPGDTPAS
LMERADLCMFAAKRAGRNRFLDDAAESLSQVA
>Mature_351_residues
SQETYSDRDRSFALAKRSHDLIRDYGPSATPRAYAVWYAYVSGELPLLADAVKRLTTQNGCLTEGDIDDLHETYLDGRRL
ASTTDDVSRVVLSEIAAVTEILDLSLGSTAQYSESLRGVAQDLSQGVPSRARFGEIVATLAATTREVAANNRILEARMRE
TRSEIETLREKLDATRRESLTDALTGLANRKHFEETLKASMETARARTTPMSLIILDIDFFKRFNDLYGHLTGDQVLRLV
AIVMRENAGKQAQLARFGGEEFGIILPGVDRAVAKQVAETVRTSVMGRELVKRSTGESLGKITISLGVAVLRPGDTPASL
MERADLCMFAAKRAGRNRFLDDAAESLSQVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=195, Percent_Identity=34.8717948717949, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787262, Length=282, Percent_Identity=29.4326241134752, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=35.3932584269663, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=34.5679012345679, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87082007, Length=226, Percent_Identity=34.070796460177, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=32.9113924050633, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=33.1325301204819, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=34.9397590361446, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788956, Length=174, Percent_Identity=32.7586206896552, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=30.9090909090909, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=188, Percent_Identity=29.7872340425532, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=30.3867403314917, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787056, Length=198, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=27.1604938271605, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38691; Mature: 38560

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQETYSDRDRSFALAKRSHDLIRDYGPSATPRAYAVWYAYVSGELPLLADAVKRLTTQN
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCC
GCLTEGDIDDLHETYLDGRRLASTTDDVSRVVLSEIAAVTEILDLSLGSTAQYSESLRGV
CCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AQDLSQGVPSRARFGEIVATLAATTREVAANNRILEARMRETRSEIETLREKLDATRRES
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDALTGLANRKHFEETLKASMETARARTTPMSLIILDIDFFKRFNDLYGHLTGDQVLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHH
VAIVMRENAGKQAQLARFGGEEFGIILPGVDRAVAKQVAETVRTSVMGRELVKRSTGESL
HHHHHHHCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
GKITISLGVAVLRPGDTPASLMERADLCMFAAKRAGRNRFLDDAAESLSQVA
HHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SQETYSDRDRSFALAKRSHDLIRDYGPSATPRAYAVWYAYVSGELPLLADAVKRLTTQN
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCC
GCLTEGDIDDLHETYLDGRRLASTTDDVSRVVLSEIAAVTEILDLSLGSTAQYSESLRGV
CCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AQDLSQGVPSRARFGEIVATLAATTREVAANNRILEARMRETRSEIETLREKLDATRRES
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDALTGLANRKHFEETLKASMETARARTTPMSLIILDIDFFKRFNDLYGHLTGDQVLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHH
VAIVMRENAGKQAQLARFGGEEFGIILPGVDRAVAKQVAETVRTSVMGRELVKRSTGESL
HHHHHHHCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
GKITISLGVAVLRPGDTPASLMERADLCMFAAKRAGRNRFLDDAAESLSQVA
HHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA