Click here to switch to the map view.

The map label for this gene is pnp [H]

Identifier: 170747372

GI number: 170747372

Start: 984191

End: 986410

Strand: Reverse

Name: pnp [H]

Synonym: Mrad2831_0939

Alternate gene names: 170747372

Gene position: 986410-984191 (Counterclockwise)

Preceding gene: 170747373

Following gene: 170747371

Centisome position: 16.23

GC content: 68.74

Gene sequence:

>2220_bases
ATGTTCGACGTTCAACGCGAAGAGCTGATGTGGGGCGACCGCAAGCTCGTCCTGGAGACCGGCAAGGTCGCCCGCCAGGC
CGACGGCGCCGTCATCGCCACCTACGGTGAGACCTCGGTGCTCGCGACCGTGGTCGCGGCCAAGGAGCCGAAGCCCGGCA
TCGACTTCATGCCGCTCACCGTGAACTACCAGGAGCGCGCCTACGCCGCCGGCCGCATCCCGGGCGGCTACTTCAAGCGC
GAGGGCCGTCCCTCCGAGAAGGAGACCCTGGTCTCCCGCCTGATCGACCGCCCGATCCGCCCGCTCTTCATCGAGGGCTG
GCGCAACGACACGCAGGTCGTCGTCACCGTCCTGACCCACGATCTCGAGAACGATCCCGACATCGTCGCGATGGTCGCGG
CCTCCGCGGCCCTGACCCTCTCGGGCGTGCCGTTCATGGGCCCGATCGGCGGCGCCCGCGTCGGCTACATCAACGGCGGC
TACCGGCTGAACCCGCTGGTCACCGAGACCAAGGAAGAGTCCAGCCTCGACCTCGTCGTCGCCGGCACGCAGGACGCGGT
GCTGATGGTCGAGTCGGAGGCCAAGGAGCTCTCCGAGGACGTGATGCTCGGCGCCGTGATGTTCGGCCACAAGCACTTCC
AGCCGGTGATCGAGGCGATCATCCGCCTCGCCGAGAAGGCCGCCAAGGAGCCGCGCGACTTCAAGGCCCCCGAGAACGCC
GACGTCGAGAAGGCCGTCCTGGAGGTCTGCGAGGCCGAGCTGCGCGCCGCCTACACCAAGACCGTCAAGCAGGAGCGCTA
CGCCGCCGTCGACGCCGTGAAGGCGAAGGTGATGGCCGCCCTCTGCCCCGAGGGCGCCGAGAAGTTCCCGGCCGAGAAGG
TCAAGGCCGCCTTCAAGGAGGCCCAGTCGAAGGTGGTCCGCTGGAACATCCTCGACAGCGGCGCCCGCATCGACGGCCGC
GACGTGAAGACGGTCCGCTCGATCCTCTCCGAGGTCGGCGTTCTGCCCCGCGCCCACGGCTCGGCCCTGTTCACCCGCGG
CGAGACCCAGGCGCTGGTCGTGGCGACGCTGGGCACCGGCGAGGACGAGCAGTTCATCGACGCGCTGGAAGGCACCTACA
AGGAGCGCTTCCTGCTTCACTACAACTTCCCTCCCTACAGCGTCGGCGAGACCGGCCGGATGGGCTCCCCGGGCCGTCGC
GAGATCGGCCACGGCAAGCTCGCCTGGCGGGCGATCCGTCCGGTCCTGCCGCCGGCCCACGAGTTCCCCTACACGATCCG
CGTCGTGTCGGAGATCACCGAGTCCAACGGCTCGTCCTCGATGGCCTCGGTCTGCGGCGGCTCGCTGTCGCTGATGGATG
CCGGCGTGCCGCTGCGCCGTCCGGTGGCGGGCATCGCCATGGGCCTCATCCTCGAGGGTGAGCGCTTCGCGGTCCTGTCC
GACATCCTCGGCGACGAGGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGCACGGACGAGGGCGTGACCTCGCTCCA
GATGGACATCAAGATCGCCGGCATCACCGAGGAGATCATGAAGATCGCCCTCGCCCAGGCGAAGGACGGCCGCGCCCACA
TCCTGGCCGAGATGGCCAAGGCCCTGACGGCGGCCCGTCCGGAGCTCGGCGAGTACGCGCCGCGCATCGAGACGATGCAG
ATCCCGACGGACAAGATCCGCGACGTGATCGGCACCGGCGGCAAGGTGATCCGCGAGATCGTCGAGAAGACCGGCGCCAA
GATCAACATCGAGGATACCGGCGTCGTGAAGATCGCCTCGGCCGACGGCAAGGCGATCAAGGCGGCCTACAACTGGATCC
GCTCGATCGTGGCGGAGCCGGAGGTCGGCGTGATCTACGACGGCACGATCGTGAAGTGCATGGAGTTCGGCGCCTTCGTG
AACTTCTTCGGCGCGAAGGACGGCCTGGTCCACATTTCGGAGCTCGCCGCCCAGCGGGTCGCCAAGGTTCAGGACGTCGT
CAAGGAAGGCGACAAGGTCAAGGTGAAGTTCCTCGGTCAGGACGACCGCGGCAAGATCCGCCTGTCCATGAAGGTCGTCG
ACCAGCAGACGGGCGAGGACATCACCGACAAGATCAAGGCGCAGCGCGACGCCGAGCGGGCCGAGCGCGGCGACGAGCCG
CGCGAGCCCCGCGAGGGCGGCCGCCACCGCGGCGAGCGCCGCCGCGAGGCCGGCGAGTAA

Upstream 100 bases:

>100_bases
GCGAGACACTTCGCCGAGGGTTCTCAGGCGCAGTGTCTCGCCGTCTTGCCCCCGGGCGGCGCCGGGCTTGGGCCCGCCGC
AGGCAAGGAAGAGATTGAGA

Downstream 100 bases:

>100_bases
GCAGGGGAACACCCGCGCCGGCTGCGGTCGGCGCGGGGTGACTCAGACGGGCGCGGTCCTCCGGGGCCGCGCCCGTTTTC
GTTCCGTCGCGGGAAGGGGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MFDVQREELMWGDRKLVLETGKVARQADGAVIATYGETSVLATVVAAKEPKPGIDFMPLTVNYQERAYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFIEGWRNDTQVVVTVLTHDLENDPDIVAMVAASAALTLSGVPFMGPIGGARVGYINGG
YRLNPLVTETKEESSLDLVVAGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFKAPENA
DVEKAVLEVCEAELRAAYTKTVKQERYAAVDAVKAKVMAALCPEGAEKFPAEKVKAAFKEAQSKVVRWNILDSGARIDGR
DVKTVRSILSEVGVLPRAHGSALFTRGETQALVVATLGTGEDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPVLPPAHEFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTDEGVTSLQMDIKIAGITEEIMKIALAQAKDGRAHILAEMAKALTAARPELGEYAPRIETMQ
IPTDKIRDVIGTGGKVIREIVEKTGAKINIEDTGVVKIASADGKAIKAAYNWIRSIVAEPEVGVIYDGTIVKCMEFGAFV
NFFGAKDGLVHISELAAQRVAKVQDVVKEGDKVKVKFLGQDDRGKIRLSMKVVDQQTGEDITDKIKAQRDAERAERGDEP
REPREGGRHRGERRREAGE

Sequences:

>Translated_739_residues
MFDVQREELMWGDRKLVLETGKVARQADGAVIATYGETSVLATVVAAKEPKPGIDFMPLTVNYQERAYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFIEGWRNDTQVVVTVLTHDLENDPDIVAMVAASAALTLSGVPFMGPIGGARVGYINGG
YRLNPLVTETKEESSLDLVVAGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFKAPENA
DVEKAVLEVCEAELRAAYTKTVKQERYAAVDAVKAKVMAALCPEGAEKFPAEKVKAAFKEAQSKVVRWNILDSGARIDGR
DVKTVRSILSEVGVLPRAHGSALFTRGETQALVVATLGTGEDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPVLPPAHEFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTDEGVTSLQMDIKIAGITEEIMKIALAQAKDGRAHILAEMAKALTAARPELGEYAPRIETMQ
IPTDKIRDVIGTGGKVIREIVEKTGAKINIEDTGVVKIASADGKAIKAAYNWIRSIVAEPEVGVIYDGTIVKCMEFGAFV
NFFGAKDGLVHISELAAQRVAKVQDVVKEGDKVKVKFLGQDDRGKIRLSMKVVDQQTGEDITDKIKAQRDAERAERGDEP
REPREGGRHRGERRREAGE
>Mature_739_residues
MFDVQREELMWGDRKLVLETGKVARQADGAVIATYGETSVLATVVAAKEPKPGIDFMPLTVNYQERAYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFIEGWRNDTQVVVTVLTHDLENDPDIVAMVAASAALTLSGVPFMGPIGGARVGYINGG
YRLNPLVTETKEESSLDLVVAGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFKAPENA
DVEKAVLEVCEAELRAAYTKTVKQERYAAVDAVKAKVMAALCPEGAEKFPAEKVKAAFKEAQSKVVRWNILDSGARIDGR
DVKTVRSILSEVGVLPRAHGSALFTRGETQALVVATLGTGEDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPVLPPAHEFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTDEGVTSLQMDIKIAGITEEIMKIALAQAKDGRAHILAEMAKALTAARPELGEYAPRIETMQ
IPTDKIRDVIGTGGKVIREIVEKTGAKINIEDTGVVKIASADGKAIKAAYNWIRSIVAEPEVGVIYDGTIVKCMEFGAFV
NFFGAKDGLVHISELAAQRVAKVQDVVKEGDKVKVKFLGQDDRGKIRLSMKVVDQQTGEDITDKIKAQRDAERAERGDEP
REPREGGRHRGERRREAGE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=711, Percent_Identity=37.9746835443038, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4826690, Length=88, Percent_Identity=51.1363636363636, Blast_Score=77, Evalue=7e-14,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=57.7424023154848, Blast_Score=769, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=118, Percent_Identity=37.2881355932203, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=701, Percent_Identity=33.3808844507846, Blast_Score=346, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17535281, Length=106, Percent_Identity=43.3962264150943, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320850, Length=91, Percent_Identity=40.6593406593407, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=37.6770538243626, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=37.6770538243626, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=37.6770538243626, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=658, Percent_Identity=37.2340425531915, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI20129977, Length=105, Percent_Identity=42.8571428571429, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 80456; Mature: 80456

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVQREELMWGDRKLVLETGKVARQADGAVIATYGETSVLATVVAAKEPKPGIDFMPLT
CCCCCHHHHHCCCCEEEEECCHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEEE
VNYQERAYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFIEGWRNDTQVVVTVLTH
ECHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEHH
DLENDPDIVAMVAASAALTLSGVPFMGPIGGARVGYINGGYRLNPLVTETKEESSLDLVV
CCCCCCCEEEHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCEECCCCCCCCEEEE
AGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFKAPENA
ECCCCEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DVEKAVLEVCEAELRAAYTKTVKQERYAAVDAVKAKVMAALCPEGAEKFPAEKVKAAFKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHH
AQSKVVRWNILDSGARIDGRDVKTVRSILSEVGVLPRAHGSALFTRGETQALVVATLGTG
HHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPVLPPAH
CCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
CCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEECCCHHHHHH
DILGDEDHLGDMDFKVAGTDEGVTSLQMDIKIAGITEEIMKIALAQAKDGRAHILAEMAK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHH
ALTAARPELGEYAPRIETMQIPTDKIRDVIGTGGKVIREIVEKTGAKINIEDTGVVKIAS
HHHHCCCCHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
ADGKAIKAAYNWIRSIVAEPEVGVIYDGTIVKCMEFGAFVNFFGAKDGLVHISELAAQRV
CCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AKVQDVVKEGDKVKVKFLGQDDRGKIRLSMKVVDQQTGEDITDKIKAQRDAERAERGDEP
HHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
REPREGGRHRGERRREAGE
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFDVQREELMWGDRKLVLETGKVARQADGAVIATYGETSVLATVVAAKEPKPGIDFMPLT
CCCCCHHHHHCCCCEEEEECCHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEEE
VNYQERAYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFIEGWRNDTQVVVTVLTH
ECHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEHH
DLENDPDIVAMVAASAALTLSGVPFMGPIGGARVGYINGGYRLNPLVTETKEESSLDLVV
CCCCCCCEEEHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCEECCCCCCCCEEEE
AGTQDAVLMVESEAKELSEDVMLGAVMFGHKHFQPVIEAIIRLAEKAAKEPRDFKAPENA
ECCCCEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DVEKAVLEVCEAELRAAYTKTVKQERYAAVDAVKAKVMAALCPEGAEKFPAEKVKAAFKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHH
AQSKVVRWNILDSGARIDGRDVKTVRSILSEVGVLPRAHGSALFTRGETQALVVATLGTG
HHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQFIDALEGTYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPVLPPAH
CCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGGSLSLMDAGVPLRRPVAGIAMGLILEGERFAVLS
CCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHEECCCHHHHHH
DILGDEDHLGDMDFKVAGTDEGVTSLQMDIKIAGITEEIMKIALAQAKDGRAHILAEMAK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHH
ALTAARPELGEYAPRIETMQIPTDKIRDVIGTGGKVIREIVEKTGAKINIEDTGVVKIAS
HHHHCCCCHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
ADGKAIKAAYNWIRSIVAEPEVGVIYDGTIVKCMEFGAFVNFFGAKDGLVHISELAAQRV
CCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AKVQDVVKEGDKVKVKFLGQDDRGKIRLSMKVVDQQTGEDITDKIKAQRDAERAERGDEP
HHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
REPREGGRHRGERRREAGE
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA