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The map label for this gene is rpoE [H]

Identifier: 170747336

GI number: 170747336

Start: 947323

End: 948027

Strand: Reverse

Name: rpoE [H]

Synonym: Mrad2831_0903

Alternate gene names: 170747336

Gene position: 948027-947323 (Counterclockwise)

Preceding gene: 170747340

Following gene: 170747335

Centisome position: 15.6

GC content: 70.21

Gene sequence:

>705_bases
ATGACTGAGGACGTTGCGGACCGGCCAGATGCAACTGCGGGCACCCGGGACGGGGCCGGCTACGCCGCGTCCATCGGCCA
GCACCTCGCCCTGCCGATCCGGGAGTATTTCAAGGTCGTCGAGCTGGAGCCCCTGCCGGCGCAGCTCGCCGCGCTGGTAT
CGCGCTTCGAGGCGGCGGTCACGGCGCACGGGGAGACGGTGCCCTTCGACTTCCGCGGCGACATCATCAAGGCGCTGCCG
GCCCTGCGCACCTTCGCCCTGTCGCTCGTGGGCGACGTGAGCCGCGCCGACGACTTGGTGCAGGAGACCTTCGTGAAGGC
CTGGGCGAACCAGGAGCGGTTCCGGCCCGGCACCAACTTCACCGCGTGGCTGTTCACGATCCTGCGCAACCAGTTCTATA
CCGATCTGCGCAAGACGCGGCGGGAGGTGGAGGACGTGGACGGGACGCATGCCGGTCAGATGACCAGCCCGTCCGATCAG
GAGGATGCCAGCACCCTCAAGGTCGTCTGGGAGCGTCTCGGCGACCTGCCGTCGGCGCAGCGCCAGGCGCTGCTGCTGGT
GGGCGCCGAGGGCCACACCTACGAGGAGGCCGCGATCATGCTCGGCTGCCAGGTCGGCACCGTGAAGAGCCGCGTGAGCC
GGGCCCGGTCGTCGCTCCTCGATACCCTCGGCGTCGTCGTGGCTGGGCAGGCTCCGGCCGCGTGA

Upstream 100 bases:

>100_bases
TTCCCTGTCACACTCGGGAGCGTTTGGCATAGGTTCAGAATTTTATCGCTACCGACGTTAGAGCTATTCTAGTATGCCAG
CGTGGGAGGCGGAAACGTTC

Downstream 100 bases:

>100_bases
TCGGCCGAGCCCCCGGCGGCACGGCCTGACGCGGCCTCGTTCCGGCGCGGGGCGCGACGCGTTCCGGCGCCGCCGCACCC
TGGACGTGGATCAACCGCAG

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MTEDVADRPDATAGTRDGAGYAASIGQHLALPIREYFKVVELEPLPAQLAALVSRFEAAVTAHGETVPFDFRGDIIKALP
ALRTFALSLVGDVSRADDLVQETFVKAWANQERFRPGTNFTAWLFTILRNQFYTDLRKTRREVEDVDGTHAGQMTSPSDQ
EDASTLKVVWERLGDLPSAQRQALLLVGAEGHTYEEAAIMLGCQVGTVKSRVSRARSSLLDTLGVVVAGQAPAA

Sequences:

>Translated_234_residues
MTEDVADRPDATAGTRDGAGYAASIGQHLALPIREYFKVVELEPLPAQLAALVSRFEAAVTAHGETVPFDFRGDIIKALP
ALRTFALSLVGDVSRADDLVQETFVKAWANQERFRPGTNFTAWLFTILRNQFYTDLRKTRREVEDVDGTHAGQMTSPSDQ
EDASTLKVVWERLGDLPSAQRQALLLVGAEGHTYEEAAIMLGCQVGTVKSRVSRARSSLLDTLGVVVAGQAPAA
>Mature_233_residues
TEDVADRPDATAGTRDGAGYAASIGQHLALPIREYFKVVELEPLPAQLAALVSRFEAAVTAHGETVPFDFRGDIIKALPA
LRTFALSLVGDVSRADDLVQETFVKAWANQERFRPGTNFTAWLFTILRNQFYTDLRKTRREVEDVDGTHAGQMTSPSDQE
DASTLKVVWERLGDLPSAQRQALLLVGAEGHTYEEAAIMLGCQVGTVKSRVSRARSSLLDTLGVVVAGQAPAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=141, Percent_Identity=32.6241134751773, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 25414; Mature: 25283

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDVADRPDATAGTRDGAGYAASIGQHLALPIREYFKVVELEPLPAQLAALVSRFEAAV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TAHGETVPFDFRGDIIKALPALRTFALSLVGDVSRADDLVQETFVKAWANQERFRPGTNF
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCH
TAWLFTILRNQFYTDLRKTRREVEDVDGTHAGQMTSPSDQEDASTLKVVWERLGDLPSAQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
RQALLLVGAEGHTYEEAAIMLGCQVGTVKSRVSRARSSLLDTLGVVVAGQAPAA
CCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEDVADRPDATAGTRDGAGYAASIGQHLALPIREYFKVVELEPLPAQLAALVSRFEAAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TAHGETVPFDFRGDIIKALPALRTFALSLVGDVSRADDLVQETFVKAWANQERFRPGTNF
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCH
TAWLFTILRNQFYTDLRKTRREVEDVDGTHAGQMTSPSDQEDASTLKVVWERLGDLPSAQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
RQALLLVGAEGHTYEEAAIMLGCQVGTVKSRVSRARSSLLDTLGVVVAGQAPAA
CCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]