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The map label for this gene is waaA [H]

Identifier: 170747322

GI number: 170747322

Start: 930922

End: 932253

Strand: Reverse

Name: waaA [H]

Synonym: Mrad2831_0889

Alternate gene names: 170747322

Gene position: 932253-930922 (Counterclockwise)

Preceding gene: 170747323

Following gene: 170747321

Centisome position: 15.34

GC content: 75.38

Gene sequence:

>1332_bases
GTGAGGCGCCCCTCCGTGACCCTGCCGCTGCAGGCCTACCGGGCCGGCCTGCGCATCGGCGAGCCGGCGCTGACCGGTCT
GCTCGCGTGGCGCGCGCAGCGCGGCAAGGAGGATCCGCGCCGGCTGCCGGAGCGGCGGGGCCTGCCGGGGCGGGCCCGGC
CGGTCGGGCCGCTCGCCTGGATGCACGGCGCCAGCGTGGGCGAGGCCCTGTCGTTGATCGGGCTCGTCGAGGGCATGATC
GCCCGCGGCTTCTCGGTCCTCGTCACCACCGGCACGCGCGCGGCCGCCGAGCTGGTCGGCGGCCGGCTGCCCCCGGGCGC
GGTGCACCAGTACATGCCCCTGGACGCGCCGCGCTGGGTCGGACGGTTCCTCGATCACTGGCAGCCGGACCTCGCGGTGA
TCGCCGAGTCGGAGATCTGGCCGAACACGATCCTGGCGCTCGACGAGCGCGAGATCCCGCTGATCCTGGTCAACGGGCGC
ATGTCGGAGCGCTCGTTCCGGGGCTGGGAGCGCTGCCCGCGCACCGCGAAGGCGCTCCTGGCGCGGATCGCGATCTGCCT
CACGCAGACCCAGGAGGACGGCGAGCGCTTCGCCAAGCTCGGCGCGCCGCGCGTGAGCATCGCCGGCAACCTCAAGTTCG
ACGCGTCGGTGCCGCCGGCCGACGCGCAGCAGCTCGCCTATCTGGGATCCATGGTGGCCGGCCGGCCGGTCTGGGTCGCG
GCGAGCACGCATCCCGGCGAGGAGGCCATGGTCGCCTACGCGCACGCGATGCTGAAGGCGCAGTTTCCCCAGCTCCTGAC
CATCATCGCCCCGCGCCACCCGGCGCGGGGCGGCGAGGCCGTCGCCTGCGCGAACGCCGTCGGCCTGCGCAGCGCGCTGC
GGTCCACCGGCGGGCGGCCCCATCCCTCGATCGAGGTCTACGTCGCCGACACGATCGGCGAGCTGGGCCTGTTCTACCGC
CTCAGCCCCCTGGTGTTCCTCGGCGGCTCCCTGGTCCCGCGGGGCGGCCAGAACCCGATCGAGCCGCTGCGTCTCGAATC
AGCGGTGCTGCACGGGCCGCACACCGAGAATTTCGGCGTGATCTACCAGGCCCTCGACCGCGCCGGCGGCGCCGTGCCGG
TGCGCGACGGCGTCGAGCTGGCGGCGGTCGCCGCCGAACTTCTCGGTGACCGGCAGCGCCTCGCCGACATGGCCCGCGCC
GGGCAGCGCGCCCTGGAGCCGTTCGAGGGGGCGGTGGCGCGCACGCTCGCGGTCCTCGACCCGTTCGTGGCGCAGATGAA
GCTCCAGCGCCTCGACCGCGGCAGCCCCGCGCGCCCGCTCGCGCGGGCCTGA

Upstream 100 bases:

>100_bases
TCCACGCCCGCGCCTACGCGCTCGTCGGGCGCGCCGACCGGGCGGCGCTCTACCGCGACCCGCTCCCCGAGACGCGGCGC
GCGCCGGTGGGAGACCTCGC

Downstream 100 bases:

>100_bases
TGCGACCGCCGGCCTTCTGGGCCGCGGGGCCGGACCACCCGGCCGCGCGCGGGCTGGGGCCGCTCGGCGCGGTCTACGGG
GCGCTGGCGGCGCGACGGAT

Product: 3-deoxy-D-manno-octulosonic-acid transferase domain-containing protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRRPSVTLPLQAYRAGLRIGEPALTGLLAWRAQRGKEDPRRLPERRGLPGRARPVGPLAWMHGASVGEALSLIGLVEGMI
ARGFSVLVTTGTRAAAELVGGRLPPGAVHQYMPLDAPRWVGRFLDHWQPDLAVIAESEIWPNTILALDEREIPLILVNGR
MSERSFRGWERCPRTAKALLARIAICLTQTQEDGERFAKLGAPRVSIAGNLKFDASVPPADAQQLAYLGSMVAGRPVWVA
ASTHPGEEAMVAYAHAMLKAQFPQLLTIIAPRHPARGGEAVACANAVGLRSALRSTGGRPHPSIEVYVADTIGELGLFYR
LSPLVFLGGSLVPRGGQNPIEPLRLESAVLHGPHTENFGVIYQALDRAGGAVPVRDGVELAAVAAELLGDRQRLADMARA
GQRALEPFEGAVARTLAVLDPFVAQMKLQRLDRGSPARPLARA

Sequences:

>Translated_443_residues
MRRPSVTLPLQAYRAGLRIGEPALTGLLAWRAQRGKEDPRRLPERRGLPGRARPVGPLAWMHGASVGEALSLIGLVEGMI
ARGFSVLVTTGTRAAAELVGGRLPPGAVHQYMPLDAPRWVGRFLDHWQPDLAVIAESEIWPNTILALDEREIPLILVNGR
MSERSFRGWERCPRTAKALLARIAICLTQTQEDGERFAKLGAPRVSIAGNLKFDASVPPADAQQLAYLGSMVAGRPVWVA
ASTHPGEEAMVAYAHAMLKAQFPQLLTIIAPRHPARGGEAVACANAVGLRSALRSTGGRPHPSIEVYVADTIGELGLFYR
LSPLVFLGGSLVPRGGQNPIEPLRLESAVLHGPHTENFGVIYQALDRAGGAVPVRDGVELAAVAAELLGDRQRLADMARA
GQRALEPFEGAVARTLAVLDPFVAQMKLQRLDRGSPARPLARA
>Mature_443_residues
MRRPSVTLPLQAYRAGLRIGEPALTGLLAWRAQRGKEDPRRLPERRGLPGRARPVGPLAWMHGASVGEALSLIGLVEGMI
ARGFSVLVTTGTRAAAELVGGRLPPGAVHQYMPLDAPRWVGRFLDHWQPDLAVIAESEIWPNTILALDEREIPLILVNGR
MSERSFRGWERCPRTAKALLARIAICLTQTQEDGERFAKLGAPRVSIAGNLKFDASVPPADAQQLAYLGSMVAGRPVWVA
ASTHPGEEAMVAYAHAMLKAQFPQLLTIIAPRHPARGGEAVACANAVGLRSALRSTGGRPHPSIEVYVADTIGELGLFYR
LSPLVFLGGSLVPRGGQNPIEPLRLESAVLHGPHTENFGVIYQALDRAGGAVPVRDGVELAAVAAELLGDRQRLADMARA
GQRALEPFEGAVARTLAVLDPFVAQMKLQRLDRGSPARPLARA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=417, Percent_Identity=39.8081534772182, Blast_Score=264, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47614; Mature: 47614

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPSVTLPLQAYRAGLRIGEPALTGLLAWRAQRGKEDPRRLPERRGLPGRARPVGPLAW
CCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCCCCHHH
MHGASVGEALSLIGLVEGMIARGFSVLVTTGTRAAAELVGGRLPPGAVHQYMPLDAPRWV
HHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCHHHH
GRFLDHWQPDLAVIAESEIWPNTILALDEREIPLILVNGRMSERSFRGWERCPRTAKALL
HHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHH
ARIAICLTQTQEDGERFAKLGAPRVSIAGNLKFDASVPPADAQQLAYLGSMVAGRPVWVA
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
ASTHPGEEAMVAYAHAMLKAQFPQLLTIIAPRHPARGGEAVACANAVGLRSALRSTGGRP
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCC
HPSIEVYVADTIGELGLFYRLSPLVFLGGSLVPRGGQNPIEPLRLESAVLHGPHTENFGV
CCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
IYQALDRAGGAVPVRDGVELAAVAAELLGDRQRLADMARAGQRALEPFEGAVARTLAVLD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
PFVAQMKLQRLDRGSPARPLARA
HHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRRPSVTLPLQAYRAGLRIGEPALTGLLAWRAQRGKEDPRRLPERRGLPGRARPVGPLAW
CCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCCCCHHH
MHGASVGEALSLIGLVEGMIARGFSVLVTTGTRAAAELVGGRLPPGAVHQYMPLDAPRWV
HHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCHHHH
GRFLDHWQPDLAVIAESEIWPNTILALDEREIPLILVNGRMSERSFRGWERCPRTAKALL
HHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHH
ARIAICLTQTQEDGERFAKLGAPRVSIAGNLKFDASVPPADAQQLAYLGSMVAGRPVWVA
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
ASTHPGEEAMVAYAHAMLKAQFPQLLTIIAPRHPARGGEAVACANAVGLRSALRSTGGRP
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCC
HPSIEVYVADTIGELGLFYRLSPLVFLGGSLVPRGGQNPIEPLRLESAVLHGPHTENFGV
CCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
IYQALDRAGGAVPVRDGVELAAVAAELLGDRQRLADMARAGQRALEPFEGAVARTLAVLD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
PFVAQMKLQRLDRGSPARPLARA
HHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]