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The map label for this gene is priA [H]

Identifier: 170747273

GI number: 170747273

Start: 880167

End: 882356

Strand: Reverse

Name: priA [H]

Synonym: Mrad2831_0840

Alternate gene names: 170747273

Gene position: 882356-880167 (Counterclockwise)

Preceding gene: 170747274

Following gene: 170747272

Centisome position: 14.52

GC content: 74.89

Gene sequence:

>2190_bases
ATGCCCGTCGTCGCCGAGATCCTGATCCCGCTCGCCCTCGATACGCCCTACAGCTACGTGGCGCCGGCCGGGCTCGACCT
CGCCGCGGGCGACGTGGTGCAGGTGCCGCTCGGCCCGCGCGAGATCGTCGGGGTCGTCTGGGGCGTCGCCGAGACGCCCG
GCGGCTCGAACCTCCGGCCGGTGACCGGCCGCCTGCCCTACCCGCCCCTCTCGGACCCGCTGCGCAGCCTCGTCGACTGG
ATCGCCCGCTACACGCTGGCGCCCAAGGGCTCGGCGCTGGCCATGGTGCTGCGGCTGCCCGACGAGGCGGCGGCCGGCGA
GACCGCCCGGGTCGCCGTGCGCCTCACGGACAAGCCCCCGTCCCGGCCGACTCCGGCCCGCACCAAGGTGCTCGCCGCGG
CGGCCGACGGCGCCCTGCGCGGCAAGAGCGCGCTCGCCAAGGAGGCCGGCGTCTCGCTCTCGGTGGTCGACGGGCTGATC
GACGACGGCGTCCTCGAGACCGTGGCGGTCGAGCCCGAGCCGGTCGCGCCGCGGCCCGACCCGGACTTCCCGCGGGCGCC
GCTCTCGCCCGCGCAGGCCGAGGCCGTGTCGGCCCTCGTCGCGCCCTTCCCGTGGGACCGGCCGCAGCCGCAGGAAGCCG
ACGATCTCCGCCCGGTCCTGCTGGAGGGCGTCACCGGCTCGGGCAAGACCGAGGTCTACTTCGAGGCGGTGGCGGCCTGC
ATCCGCGCCGGGCGCCAAGCACTGATCCTGATGCCCGAGATCGCCCTGACGGCGCAGTTCCTCGACCGGTTCGCCGCGCG
GTTCGGGGTCCGGCCGGCCGCGTGGCATTCGGGGATCGGCGGCAGGCGGCGCGAGCGCCTGCGCGCCGCCGTGGCCGAGG
GCGAGGCGCTCGTTGTGGTCGGCGCGCGCTCGGCCCTGTTCCTGCCCTTCGCGCGGCTCGGCCTGATCGTGGTCGATGAG
GAGCACGAGACCGCCTACAAGCAGGAGGACGGCGTCCATTACCACGCCCGCGACATGGCGGTCGTGCGCGGACGCCTCGA
GGGCTGCCCGGTCGTCCTGACCTCGGCGACGCCCGCCATCGAGACCCGCGTCAACGCCGCGCGGGGGCGCTACCGGCACG
TCCTGCTCCCTGAGCGCTTCGGCGGGCGGCGCCTGCCCGACATCGCCGCCATCGACATGCGCCTCGACCAGCCCGAGCGG
GGCCGCTTCCTGAGCCCGCCGCTGGTCAACGCCGTGAAGAGCACCCTGGAGCGCGGCGAGCAGGCGCTGCTGTTCCTCAA
CCGCCGGGGCTACGCGCCGCTGACCCTCTGCCGGGCCTGCGGCCACCGCTACCAGTGCAAGAACTGCTCGACCTGGCTCG
TGGAGCACCGGTTCCGCCGGGCGCTGGTCTGCCACCAGTGCGGCTACGCGGAGCGCCGGCCGGAGGCCTGCACCGAGTGC
GGGACCTTCGACAATCTCACCGCGTGCGGGCCCGGCGTCGAGCGGATCGCCGAAGAGGCCGCTGAGATGTTCCCCGACCG
GCGCATCGTCGTCCTGTCGAGCGACTTCCCCGGCGGCGCCGAGCGTCTGCGCCAGGAACTCGACGCCGTGGCGGCGGGCG
AGTGCGACGTCGTGATCGGCACGCAGCTCGTGGCCAAGGGCCACAACTTCCCGTTCCTGACCCTGGTCGGCGTCCTCGAC
GCCGATATCGGCCTGACATCGGGCGACCCGCGGGCAGCCGAGCGGACTTTCCAGCTGCTCCAGCAGGTCACCGGGCGCGC
CGGACGCGGCGAGAAGCCCGGGCGCGCCCTGGTCCAGACCTACCAGCCGGACCACCCGGTGATCGCGGCGCTCCTCTCGG
GCGACGCCGAGCGCTTCTACGCGGAGGAGATCCAGGCCCGCGAGGCGGCCGGCCTGCCGCCGTTCGGGCGGCTCGCGGCC
CTGATCGTCTCGGCGAGCGAGCGCGAGGTCGCGGAGGCGCACGGTCAGGCCTTGGCCCGCGCCGCCGAGCCGCCGGAGGG
CGTGATGGTGCTGGGGCCCGCCGAGGCGCCGCTCGCCCTGGTGCGCGGCCGCTACCGCTTCCGGCTGCTCGTGAAGACCG
AGCGCAACGTCGATCTCCAGGCCTACCTGCGCGCCTGGATCGCCCGGGGCCCGAAGGTGCGGGGCAACGTGCGGGTCGCC
ATTGATGTCGACCCGCAGAGCTTTTTGTAG

Upstream 100 bases:

>100_bases
CCCTGCCGCTCGTGCCGGCGCTGCGGGATCGCTGAAGCGGCGGGTTCTTTTCCCGCGCCTATGGTACGGACCCGGAACGG
ACCCGCCCGAAAAGCCCCGC

Downstream 100 bases:

>100_bases
GAGACTTCGCGGACCCCCCCGGCCTTGTGAACGCCGGTTCGCCGTCGGGAGGCACGTCAGAGCGAGGGTCCGGGGCCATA
CCGGTTCGTCCCGCGATCGG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MPVVAEILIPLALDTPYSYVAPAGLDLAAGDVVQVPLGPREIVGVVWGVAETPGGSNLRPVTGRLPYPPLSDPLRSLVDW
IARYTLAPKGSALAMVLRLPDEAAAGETARVAVRLTDKPPSRPTPARTKVLAAAADGALRGKSALAKEAGVSLSVVDGLI
DDGVLETVAVEPEPVAPRPDPDFPRAPLSPAQAEAVSALVAPFPWDRPQPQEADDLRPVLLEGVTGSGKTEVYFEAVAAC
IRAGRQALILMPEIALTAQFLDRFAARFGVRPAAWHSGIGGRRRERLRAAVAEGEALVVVGARSALFLPFARLGLIVVDE
EHETAYKQEDGVHYHARDMAVVRGRLEGCPVVLTSATPAIETRVNAARGRYRHVLLPERFGGRRLPDIAAIDMRLDQPER
GRFLSPPLVNAVKSTLERGEQALLFLNRRGYAPLTLCRACGHRYQCKNCSTWLVEHRFRRALVCHQCGYAERRPEACTEC
GTFDNLTACGPGVERIAEEAAEMFPDRRIVVLSSDFPGGAERLRQELDAVAAGECDVVIGTQLVAKGHNFPFLTLVGVLD
ADIGLTSGDPRAAERTFQLLQQVTGRAGRGEKPGRALVQTYQPDHPVIAALLSGDAERFYAEEIQAREAAGLPPFGRLAA
LIVSASEREVAEAHGQALARAAEPPEGVMVLGPAEAPLALVRGRYRFRLLVKTERNVDLQAYLRAWIARGPKVRGNVRVA
IDVDPQSFL

Sequences:

>Translated_729_residues
MPVVAEILIPLALDTPYSYVAPAGLDLAAGDVVQVPLGPREIVGVVWGVAETPGGSNLRPVTGRLPYPPLSDPLRSLVDW
IARYTLAPKGSALAMVLRLPDEAAAGETARVAVRLTDKPPSRPTPARTKVLAAAADGALRGKSALAKEAGVSLSVVDGLI
DDGVLETVAVEPEPVAPRPDPDFPRAPLSPAQAEAVSALVAPFPWDRPQPQEADDLRPVLLEGVTGSGKTEVYFEAVAAC
IRAGRQALILMPEIALTAQFLDRFAARFGVRPAAWHSGIGGRRRERLRAAVAEGEALVVVGARSALFLPFARLGLIVVDE
EHETAYKQEDGVHYHARDMAVVRGRLEGCPVVLTSATPAIETRVNAARGRYRHVLLPERFGGRRLPDIAAIDMRLDQPER
GRFLSPPLVNAVKSTLERGEQALLFLNRRGYAPLTLCRACGHRYQCKNCSTWLVEHRFRRALVCHQCGYAERRPEACTEC
GTFDNLTACGPGVERIAEEAAEMFPDRRIVVLSSDFPGGAERLRQELDAVAAGECDVVIGTQLVAKGHNFPFLTLVGVLD
ADIGLTSGDPRAAERTFQLLQQVTGRAGRGEKPGRALVQTYQPDHPVIAALLSGDAERFYAEEIQAREAAGLPPFGRLAA
LIVSASEREVAEAHGQALARAAEPPEGVMVLGPAEAPLALVRGRYRFRLLVKTERNVDLQAYLRAWIARGPKVRGNVRVA
IDVDPQSFL
>Mature_728_residues
PVVAEILIPLALDTPYSYVAPAGLDLAAGDVVQVPLGPREIVGVVWGVAETPGGSNLRPVTGRLPYPPLSDPLRSLVDWI
ARYTLAPKGSALAMVLRLPDEAAAGETARVAVRLTDKPPSRPTPARTKVLAAAADGALRGKSALAKEAGVSLSVVDGLID
DGVLETVAVEPEPVAPRPDPDFPRAPLSPAQAEAVSALVAPFPWDRPQPQEADDLRPVLLEGVTGSGKTEVYFEAVAACI
RAGRQALILMPEIALTAQFLDRFAARFGVRPAAWHSGIGGRRRERLRAAVAEGEALVVVGARSALFLPFARLGLIVVDEE
HETAYKQEDGVHYHARDMAVVRGRLEGCPVVLTSATPAIETRVNAARGRYRHVLLPERFGGRRLPDIAAIDMRLDQPERG
RFLSPPLVNAVKSTLERGEQALLFLNRRGYAPLTLCRACGHRYQCKNCSTWLVEHRFRRALVCHQCGYAERRPEACTECG
TFDNLTACGPGVERIAEEAAEMFPDRRIVVLSSDFPGGAERLRQELDAVAAGECDVVIGTQLVAKGHNFPFLTLVGVLDA
DIGLTSGDPRAAERTFQLLQQVTGRAGRGEKPGRALVQTYQPDHPVIAALLSGDAERFYAEEIQAREAAGLPPFGRLAAL
IVSASEREVAEAHGQALARAAEPPEGVMVLGPAEAPLALVRGRYRFRLLVKTERNVDLQAYLRAWIARGPKVRGNVRVAI
DVDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=748, Percent_Identity=33.9572192513369, Blast_Score=356, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 78564; Mature: 78433

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVAEILIPLALDTPYSYVAPAGLDLAAGDVVQVPLGPREIVGVVWGVAETPGGSNLRP
CCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCC
VTGRLPYPPLSDPLRSLVDWIARYTLAPKGSALAMVLRLPDEAAAGETARVAVRLTDKPP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHCCCCCEEEEEEECCCCC
SRPTPARTKVLAAAADGALRGKSALAKEAGVSLSVVDGLIDDGVLETVAVEPEPVAPRPD
CCCCCHHHHEEEECCCCCCCCHHHHHHHHCCCCEEHHHHHCCCCHHEEEECCCCCCCCCC
PDFPRAPLSPAQAEAVSALVAPFPWDRPQPQEADDLRPVLLEGVTGSGKTEVYFEAVAAC
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHH
IRAGRQALILMPEIALTAQFLDRFAARFGVRPAAWHSGIGGRRRERLRAAVAEGEALVVV
HHCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
GARSALFLPFARLGLIVVDEEHETAYKQEDGVHYHARDMAVVRGRLEGCPVVLTSATPAI
ECCHHHHHHHHHCCEEEEECCHHHHHHCCCCCEEEHHHHHHHHHCCCCCCEEEECCCCHH
ETRVNAARGRYRHVLLPERFGGRRLPDIAAIDMRLDQPERGRFLSPPLVNAVKSTLERGE
HHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
QALLFLNRRGYAPLTLCRACGHRYQCKNCSTWLVEHRFRRALVCHQCGYAERRPEACTEC
EEEEEEECCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
GTFDNLTACGPGVERIAEEAAEMFPDRRIVVLSSDFPGGAERLRQELDAVAAGECDVVIG
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEC
TQLVAKGHNFPFLTLVGVLDADIGLTSGDPRAAERTFQLLQQVTGRAGRGEKPGRALVQT
CHHEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
YQPDHPVIAALLSGDAERFYAEEIQAREAAGLPPFGRLAALIVSASEREVAEAHGQALAR
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
AAEPPEGVMVLGPAEAPLALVRGRYRFRLLVKTERNVDLQAYLRAWIARGPKVRGNVRVA
CCCCCCCEEEECCCCCCHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEE
IDVDPQSFL
EECCCHHCC
>Mature Secondary Structure 
PVVAEILIPLALDTPYSYVAPAGLDLAAGDVVQVPLGPREIVGVVWGVAETPGGSNLRP
CHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCC
VTGRLPYPPLSDPLRSLVDWIARYTLAPKGSALAMVLRLPDEAAAGETARVAVRLTDKPP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHCCCCCEEEEEEECCCCC
SRPTPARTKVLAAAADGALRGKSALAKEAGVSLSVVDGLIDDGVLETVAVEPEPVAPRPD
CCCCCHHHHEEEECCCCCCCCHHHHHHHHCCCCEEHHHHHCCCCHHEEEECCCCCCCCCC
PDFPRAPLSPAQAEAVSALVAPFPWDRPQPQEADDLRPVLLEGVTGSGKTEVYFEAVAAC
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHH
IRAGRQALILMPEIALTAQFLDRFAARFGVRPAAWHSGIGGRRRERLRAAVAEGEALVVV
HHCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
GARSALFLPFARLGLIVVDEEHETAYKQEDGVHYHARDMAVVRGRLEGCPVVLTSATPAI
ECCHHHHHHHHHCCEEEEECCHHHHHHCCCCCEEEHHHHHHHHHCCCCCCEEEECCCCHH
ETRVNAARGRYRHVLLPERFGGRRLPDIAAIDMRLDQPERGRFLSPPLVNAVKSTLERGE
HHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
QALLFLNRRGYAPLTLCRACGHRYQCKNCSTWLVEHRFRRALVCHQCGYAERRPEACTEC
EEEEEEECCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
GTFDNLTACGPGVERIAEEAAEMFPDRRIVVLSSDFPGGAERLRQELDAVAAGECDVVIG
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEC
TQLVAKGHNFPFLTLVGVLDADIGLTSGDPRAAERTFQLLQQVTGRAGRGEKPGRALVQT
CHHEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
YQPDHPVIAALLSGDAERFYAEEIQAREAAGLPPFGRLAALIVSASEREVAEAHGQALAR
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
AAEPPEGVMVLGPAEAPLALVRGRYRFRLLVKTERNVDLQAYLRAWIARGPKVRGNVRVA
CCCCCCCEEEECCCCCCHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEE
IDVDPQSFL
EECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]