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The map label for this gene is prlC

Identifier: 170684269

GI number: 170684269

Start: 3854565

End: 3856607

Strand: Reverse

Name: prlC

Synonym: EcSMS35_3787

Alternate gene names: 170684269

Gene position: 3856607-3854565 (Counterclockwise)

Preceding gene: 170680664

Following gene: 170683366

Centisome position: 76.09

GC content: 55.41

Gene sequence:

>2043_bases
ATGACGAATCCGTTACTGACTCCCTTTGAATTGCCTCCGTTTTCTAAAATTCTCCCGGAACATGTCGTTCCAGCCGTGAC
TAAGGCGCTGAACGACTGCCGCGAAAATGTGGAGCGCGTAGTAGCGCAAGGGGCACCGTACACCTGGGAAAATCTCTGCC
AGCCGTTGGCGGAAGTGGACGATGTGCTGGGGCGTATCTTCTCCCCGGTCAGCCACCTGAACTCGGTGAAAAATAGCCCG
GAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGAATACAGCACCTGGGTAGGGCAACATGAAGGGCTGTA
TAAGGCATATCGCGACCTGCGCGATGGCGATCATTACGCCACGCTGAACACGGCGCAGAAAAAAGCGGTTGATAACGCAC
TGCGCGACTTCGAACTCTCTGGCATCGGTCTGCCGAAAGAGAAACAGCAGCGTTACGGCGAAATTGCTACCCGTCTTTCT
GAACTGGGCAACCAGTACAGCAACAACGTCCTCGATGCGACGATGGGCTGGACCAAACTCGTTACCGACGAAGCGGAGCT
GGCGGGGATGCCAGAAAGCGCGCTGGCTGCGGCAAAAGCCCAGGCCGAAGCGAAAGAGCTGGAAGGTTATTTGCTGACGC
TGGATATCCCAAGCTACTTGCCGGTAATGACCTACTGCGACAACCAGGCTCTGCGTGAAGAGATGTATCGTGCTTACAGC
ACCCGCGCTTCCGATCAAGGCCCGAACGCCGGTAAGTGGGACAACAGCAAGGTGATGGAAGAGATCCTCGCGCTGCGTCA
CGAACTGGCGCAACTGCTGGGCTTTGAAAACTATGCCTTTAAATCCCTTGCTACTAAAATGGCAGAAAACCCGCAGCAGG
TGCTGGATTTCTTAACCGATCTGGCAAAACGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAACTGCGTGCCTTCGCC
AAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAGAAACAGCACCTCTACAG
CATCAGCGATGAACAACTGCGTCCGTACTTCCCGGAAAACAAAGCGGTTAACGGCCTGTTTGAAGTGGTGAAACGTATTT
ACGGCATCACCGCTAAAGAGCGTAAAGATGTTGATGTCTGGCATCCGGATGTACGTTTCTTCGAACTGTATGACGAGAAC
AACGAACTGCGCGGCAGCTTCTACCTCGACCTGTATGCCCGTGAAAACAAACGCGGCGGGGCGTGGATGGATGACTGCGT
AGGCCAGATGCGTAAAGCCGACGGTTCGCTGCAAAAACCGGTCGCGTATCTGACCTGCAACTTCAACCGCCCGGTAAATG
GTAAACCGGCGCTGTTTACCCATGACGAAGTGATCACCCTGTTCCACGAGTTCGGTCACGGCCTGCATCATATGCTGACC
CGCATTGAAACCGCTGGAGTGTCTGGTATCAGCGGGGTGCCGTGGGATGCGGTCGAACTGCCGAGCCAGTTTATGGAAAA
CTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCACTATGAAACCGGCGAACCGCTGCCGAAAGAGTTGCTGG
ATAAAATGCTGGCGGCGAAGAACTACCAGGCGGCGCTGTTTATTCTGCGCCAGCTGGAGTTCGGTCTGTTCGATTTCCGC
CTCCATGCCGAGTTCCGCCCGGATCAGGGAGCGAAAATCCTCGAAACTCTGGCAGAAATCAAGAAACTGGTTGCCGTAGT
ACCGTCTCCATCCTGGGGCCGTTTCCCGCACGCTTTCAGCCATATTTTCGCCGGTGGTTATGCCGCAGGTTACTACAGCT
ACCTGTGGGCCGACGTGCTGGCGGCAGATGCCTTCTCGCGCTTTGAGGAAGAGGGCATTTTCAACCGTGAAACCGGACAG
TCGTTCCTCGACAACATTCTGAGCCGTGGCGGTTCAGAAGAGCCGATGGATCTGTTCAAACGCTTCCGTGGTCGTGAACC
GCAGCTGGATGCGATGCTGGAGCATTACGGCATTAAGGGCTGA

Upstream 100 bases:

>100_bases
CGTTTCTCATTGAAATTCACTACACTTAACCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTCCTTAACCT
CTTTAACCAGGACTGCGCTA

Downstream 100 bases:

>100_bases
TCATTCAGTGAAAATCTGCTTAATTGATGAAACAGGCACCGGAGACGGTGCCTTATCTGTTCTGGCGGCCCGCTGGGGGC
TGGAGCACGATGAAGACAAC

Product: oligopeptidase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQGAPYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSP
ELREAYEQTLPLLSEYSTWVGQHEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPSYLPVMTYCDNQALREEMYRAYS
TRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFENYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFA
KAEFGVDELQPWDIAYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVRFFELYDEN
NELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNRPVNGKPALFTHDEVITLFHEFGHGLHHMLT
RIETAGVSGISGVPWDAVELPSQFMENWCWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFR
LHAEFRPDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADAFSRFEEEGIFNRETGQ
SFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG

Sequences:

>Translated_680_residues
MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQGAPYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSP
ELREAYEQTLPLLSEYSTWVGQHEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPSYLPVMTYCDNQALREEMYRAYS
TRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFENYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFA
KAEFGVDELQPWDIAYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVRFFELYDEN
NELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNRPVNGKPALFTHDEVITLFHEFGHGLHHMLT
RIETAGVSGISGVPWDAVELPSQFMENWCWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFR
LHAEFRPDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADAFSRFEEEGIFNRETGQ
SFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG
>Mature_679_residues
TNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQGAPYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPE
LREAYEQTLPLLSEYSTWVGQHEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLSE
LGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPSYLPVMTYCDNQALREEMYRAYST
RASDQGPNAGKWDNSKVMEEILALRHELAQLLGFENYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAK
AEFGVDELQPWDIAYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVRFFELYDENN
ELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNRPVNGKPALFTHDEVITLFHEFGHGLHHMLTR
IETAGVSGISGVPWDAVELPSQFMENWCWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRL
HAEFRPDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADAFSRFEEEGIFNRETGQS
FLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG

Specific function: May play a specific role in the degradation of signal peptides after they are released from precursor forms of secreted proteins. Can cleave N-acetyl-L-Ala(4)

COG id: COG0339

COG function: function code E; Zn-dependent oligopeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M3 family

Homologues:

Organism=Homo sapiens, GI4507491, Length=640, Percent_Identity=31.5625, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI14149738, Length=642, Percent_Identity=31.4641744548287, Blast_Score=292, Evalue=9e-79,
Organism=Homo sapiens, GI156105687, Length=618, Percent_Identity=27.0226537216829, Blast_Score=206, Evalue=7e-53,
Organism=Escherichia coli, GI1789913, Length=680, Percent_Identity=100, Blast_Score=1411, Evalue=0.0,
Organism=Escherichia coli, GI1787819, Length=687, Percent_Identity=32.4599708879185, Blast_Score=327, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI71999758, Length=574, Percent_Identity=25.7839721254355, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32565901, Length=637, Percent_Identity=23.861852433281, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319793, Length=691, Percent_Identity=28.7988422575977, Blast_Score=299, Evalue=9e-82,
Organism=Saccharomyces cerevisiae, GI6322715, Length=697, Percent_Identity=23.3859397417504, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21356111, Length=560, Percent_Identity=30.3571428571429, Blast_Score=255, Evalue=7e-68,
Organism=Drosophila melanogaster, GI20129717, Length=614, Percent_Identity=25.5700325732899, Blast_Score=192, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPDA_ECOLI (P27298)

Other databases:

- EMBL:   M93984
- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S47718
- RefSeq:   AP_004295.1
- RefSeq:   NP_417955.1
- ProteinModelPortal:   P27298
- SMR:   P27298
- DIP:   DIP-10566N
- MINT:   MINT-1234260
- STRING:   P27298
- MEROPS:   M03.004
- EnsemblBacteria:   EBESCT00000002212
- EnsemblBacteria:   EBESCT00000017084
- GeneID:   948016
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3465
- KEGG:   eco:b3498
- EchoBASE:   EB1411
- EcoGene:   EG11441
- eggNOG:   COG0339
- GeneTree:   EBGT00050000008965
- HOGENOM:   HBG678447
- OMA:   WSPVSHL
- ProtClustDB:   PRK10911
- BioCyc:   EcoCyc:EG11441-MONOMER
- Genevestigator:   P27298
- GO:   GO:0006508
- InterPro:   IPR001567

Pfam domain/function: PF01432 Peptidase_M3

EC number: =3.4.24.70

Molecular weight: Translated: 77168; Mature: 77037

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 470-470

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQGAPYTWENLCQPLAEVD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQHEGLYKAYRDLRDGDHYA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
TLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLSELGNQYSNNVLDATMGWTKL
EHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHH
VTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPSYLPVMTYCDNQALREEMYRAYS
HCCCHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHH
TRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFENYAFKSLATKMAENPQQVLDFLTD
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHH
LAKRARPQGEKELAQLRAFAKAEFGVDELQPWDIAYYSEKQKQHLYSISDEQLRPYFPEN
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCC
KAVNGLFEVVKRIYGITAKERKDVDVWHPDVRFFELYDENNELRGSFYLDLYARENKRGG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCC
AWMDDCVGQMRKADGSLQKPVAYLTCNFNRPVNGKPALFTHDEVITLFHEFGHGLHHMLT
CCHHHHHHHHHHCCCCHHCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RIETAGVSGISGVPWDAVELPSQFMENWCWEPEALAFISGHYETGEPLPKELLDKMLAAK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHC
NYQAALFILRQLEFGLFDFRLHAEFRPDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFS
CHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HIFAGGYAAGYYSYLWADVLAADAFSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
RFRGREPQLDAMLEHYGIKG
HHCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQGAPYTWENLCQPLAEVD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQHEGLYKAYRDLRDGDHYA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
TLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLSELGNQYSNNVLDATMGWTKL
EHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHH
VTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPSYLPVMTYCDNQALREEMYRAYS
HCCCHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHH
TRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFENYAFKSLATKMAENPQQVLDFLTD
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHH
LAKRARPQGEKELAQLRAFAKAEFGVDELQPWDIAYYSEKQKQHLYSISDEQLRPYFPEN
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCC
KAVNGLFEVVKRIYGITAKERKDVDVWHPDVRFFELYDENNELRGSFYLDLYARENKRGG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCC
AWMDDCVGQMRKADGSLQKPVAYLTCNFNRPVNGKPALFTHDEVITLFHEFGHGLHHMLT
CCHHHHHHHHHHCCCCHHCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RIETAGVSGISGVPWDAVELPSQFMENWCWEPEALAFISGHYETGEPLPKELLDKMLAAK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHC
NYQAALFILRQLEFGLFDFRLHAEFRPDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFS
CHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HIFAGGYAAGYYSYLWADVLAADAFSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
RFRGREPQLDAMLEHYGIKG
HHCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1325967; 8366062; 8041620; 9278503