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The map label for this gene is fdoI [H]

Identifier: 170684266

GI number: 170684266

Start: 4377906

End: 4378541

Strand: Reverse

Name: fdoI [H]

Synonym: EcSMS35_4278

Alternate gene names: 170684266

Gene position: 4378541-4377906 (Counterclockwise)

Preceding gene: 170680015

Following gene: 170681666

Centisome position: 86.39

GC content: 52.67

Gene sequence:

>636_bases
ATGAAACGACGTGACACCATCGTGCGCTACACCGCGCCGGAACGTATCAACCACTGGATCACCGCCTTCTGCTTCATCCT
GGCGGCGGTGAGCGGGCTGGGCTTTTTGTTCCCGTCCTTCAACTGGTTGATGCAAATCATGGGCACATCGCAGCTGGCGC
GCATTCTGCACCCGTTTGTCGGCGTGGTTATGTTTGCCTCGTTCATCATCATGTTTTTCCGTTACTGGCATCACAACCTA
ATCAATCGGGATGATATCTTTTGGGCGAAGAATATTCGTAAGATCGTCGTCAACGAGGAAGTAGGCGACACCGGGCGTTA
TAACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGC
GTCCTTATTTTGCGCCTGCTTTCTCAATCCCGGTGATCCGATTCGCGTTAATGCTGCATTCATTTGCCGCAGTGGCGTTA
ATTGTGGTTATCATGGTGCATATCTACGCCGCCCTTTGGGTGAAAGGCACGATTACCGCCATGGTGGAAGGATGGGTTAC
CAGCGCCTGGGCGAAGAAACATCACCCGCGCTGGTACCGTGAGGTCCGCAAGACAACGGAAAAGAAAGCTGAATGA

Upstream 100 bases:

>100_bases
CTACCTTCGCAGCCAGTATCTTCCACTACGTGGGTGTCGGTCCGAACCGTGCGGATGAGGAAGAGAATAATCTGCACGAA
GAGAAAGACGAGGAGCGCAA

Downstream 100 bases:

>100_bases
GTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCT
CGGCTCAAGAATTTATACAA

Product: formate dehydrogenase-O subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTSQLARILHPFVGVVMFASFIIMFFRYWHHNL
INRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE

Sequences:

>Translated_211_residues
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTSQLARILHPFVGVVMFASFIIMFFRYWHHNL
INRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
>Mature_211_residues
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTSQLARILHPFVGVVMFASFIIMFFRYWHHNL
INRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=211, Percent_Identity=99.5260663507109, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787750, Length=213, Percent_Identity=47.4178403755869, Blast_Score=210, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24597; Mature: 24597

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTSQLARILHPFV
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
FLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVALIVVIMVHIYAALWVKGTITA
HHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTSQLARILHPFV
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAII
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
FLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVALIVVIMVHIYAALWVKGTITA
HHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]