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The map label for this gene is mlaE

Identifier: 170684208

GI number: 170684208

Start: 3565079

End: 3565861

Strand: Reverse

Name: mlaE

Synonym: EcSMS35_3490

Alternate gene names: 170684208

Gene position: 3565861-3565079 (Counterclockwise)

Preceding gene: 170683530

Following gene: 170682506

Centisome position: 70.35

GC content: 55.43

Gene sequence:

>783_bases
ATGCTGTTAAATGCGCTGGCGTCGCTCGGACATAAAGGGATTAAAACCCTGAGAACGTTCGGGCGGGCCGGGTTAATGTT
ATTCAATGCGCTGGTCGGCAAACCGGAATTTCGCAAACATGCGCCGCTGCTGGTGCGCCAGCTCTATAATGTCGGCGTCC
TGTCGATGCTGATTATTGTAGTTTCTGGCGTGTTCATCGGAATGGTGTTGGGGCTGCAAGGTTATCTGGTTCTGACCACT
TATAGTGCGGAAACCAGTCTGGGTATGCTGGTGGCGTTATCGCTACTGCGTGAACTGGGGCCGGTGGTTGCCGCGCTGTT
GTTTGCCGGGCGTGCCGGTTCGGCGCTAACCGCAGAAATCGGCCTGATGCGCGCTACAGAGCAACTCTCCAGTATGGAGA
TGATGGCGGTGGATCCGCTGCGTCGGGTTATTTCTCCCCGTTTCTGGGCTGGGGTTATTTCATTACCACTGTTGACGGTT
ATCTTCGTCGCCGTGGGGATCTGGGGCGGATCGCTGGTCGGCGTCAGTTGGAAAGGCATTGATAGCGGGTTCTTCTGGTC
GGCAATGCAAAATGCCGTCGACTGGCGTATGGATCTGGTCAACTGTCTGATTAAGAGCGTGGTGTTTGCCATCACGGTGA
CGTGGATTTCGTTGTTTAACGGCTACGACGCCATCCCGACGTCTGCCGGGATTAGCCGGGCAACCACTCGCACCGTTGTC
CACTCGTCTCTGGCTGTTCTGGGGCTGGATTTTGTGCTGACCGCATTGATGTTTGGGAATTGA

Upstream 100 bases:

>100_bases
GTACGTCAGTTTCTGGACGGGATAGCTGACGGGCCTGTTCCGTTCCGCTATCCTGCCGGCGATTATCACGCTGATCTTTT
ACCAGGGAGTTAAGCCACTC

Downstream 100 bases:

>100_bases
GTTCATGCAAACGAAAAAAAATGAAATTTGGGTGGGTATCTTTTTATTAGCAGCACTGCTTGCGGCGCTTTTTGTTTGCC
TGAAGGCAGCGAACGTGACG

Product: toluene ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLLVRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISLFNGYDAIPTSAGISRATTRTVV
HSSLAVLGLDFVLTALMFGN

Sequences:

>Translated_260_residues
MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLLVRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISLFNGYDAIPTSAGISRATTRTVV
HSSLAVLGLDFVLTALMFGN
>Mature_260_residues
MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLLVRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISLFNGYDAIPTSAGISRATTRTVV
HSSLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family

Homologues:

Organism=Escherichia coli, GI1789585, Length=260, Percent_Identity=100, Blast_Score=511, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLAE_ECO57 (P64608)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91138
- PIR:   D85983
- RefSeq:   NP_289768.1
- RefSeq:   NP_312100.1
- ProteinModelPortal:   P64608
- EnsemblBacteria:   EBESCT00000024016
- EnsemblBacteria:   EBESCT00000058442
- GeneID:   916084
- GeneID:   958636
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4557
- KEGG:   ecs:ECs4073
- GeneTree:   EBGT00050000009860
- HOGENOM:   HBG564941
- OMA:   LNAMAIR
- ProtClustDB:   CLSK880619
- BioCyc:   ECOL83334:ECS4073-MONOMER
- GO:   GO:0006810
- InterPro:   IPR003453
- TIGRFAMs:   TIGR00056

Pfam domain/function: PF02405 DUF140

EC number: NA

Molecular weight: Translated: 27864; Mature: 27864

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1bce9f30)-; HASH(0x1c7cb334)-; HASH(0x1c08d078)-; HASH(0x1a90acbc)-; HASH(0x1c7efe4c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLLVRQLYNVGVLSMLIIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTVIFVAVGIWGGSLVGVSWKGI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISLFNGYDAIPTSAGISRATTRTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLLVRQLYNVGVLSMLIIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTVIFVAVGIWGGSLVGVSWKGI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISLFNGYDAIPTSAGISRATTRTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796