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The map label for this gene is rlmG [H]

Identifier: 170684079

GI number: 170684079

Start: 3459537

End: 3460673

Strand: Reverse

Name: rlmG [H]

Synonym: EcSMS35_3376

Alternate gene names: 170684079

Gene position: 3460673-3459537 (Counterclockwise)

Preceding gene: 170679962

Following gene: 170681948

Centisome position: 68.28

GC content: 53.65

Gene sequence:

>1137_bases
ATGAGCCACTTAGACAACGGTTTCCGTTCACTGACACTACAACGTTTTCCGGCGACGGATGACGTTAACCCGCTACAGGC
GTGGGAAGCGGCGGATGAATATTTGCTGCAACAGTTGGACGACACAGAAATCCGCGGCCCGGTGTTGATCCTGAATGATG
CCTTTGGTGCGTTAAGTTGTGCGCTGGCGGAACATAAGCCGTACAGCATTGGCGACTCATACATCAGTGAACTGGCCACG
CGCGAGAATTTACGCCTCAACGGGATTGATGAATCGAGCGTGAAGTTTCTCGACAGCACCGCCGACTACCCGCAACAGCC
GGGCGTGGTACTGATCAAAGTACCGAAAACACTGGCGTTGCTGGAACAACAACTGCGTGCGCTGCGCAAAGTGGTCACGC
CGGATACACGTATTATTGCCGGTGCTAAAGCCCGTGACATTCACACCTCCACGCTGGAACTGTTCGAAAAAGTGCTCGGT
CCGACCACCACCACACTGGCATGGAAGAAAGCGCGCCTGATTAACTGCACTTTCAACGAGCCGCCGCTGGTTGATGCACC
GCAGACCGTTAGCTGGAAGCTGGAAGGTACTGACTGGACTATCCACAACCATGCGAATGTCTTCTCCCGCACCGGGCTGG
ATATTGGCGCGCGCTTCTTTATGCAGCATCTGCCAGAGAATCTCGAAGGTGAGATTGTCGATCTCGGTTGTGGTAATGGC
GTTATTGGTCTGACGCTGCTTGATAAAAACCCGCAGGCGAAAGTGGTGTTTGTCGATGAATCGCCGATGGCGGTTGCTTC
CAGCCGTTTGAACGTTGAAACCAACATGCCAGAGGCGTTGGATCGCTGCGAGTTTATGATTAACAACGCGCTCTCCGGCG
TGGAGCCTTTCCGCTTTAATGCTGTGCTCTGCAACCCGCCGTTTCATCAGCAACATGCGCTGACCGATAACGTCGCCTGG
GAGATGTTCCACCACGCCCGCCGCTGTCTGAAAATCAACGGCGAGCTGTATATCGTTGCCAACCGTCACCTGGATTACTT
CCACAAACTGAAGAAAATTTTCGGCAACTGCACCACCATCGCCACGAATAATAAATTTGTGGTGCTGAAAGCAGTGAAGC
TGGGGCGTCGTCGGTAA

Upstream 100 bases:

>100_bases
AAGTAAGATTGCTCATAACCGCCCACGAAAAAGAGAAAAAGGGTATACTCACGCACCCTTTTCAGGGGCAACGTCGAAAT
TTTATCATTCAGGAGGGCCG

Downstream 100 bases:

>100_bases
GATTGGCAGCTACCTGCCAGATAAGGCGTAAACGCCTTATCTGGCATACGTTCAGCACATTACCCCCAGGTCGGATAAGA
CGCGGCAAGCGTCGCATCCG

Product: methyltransferase family protein

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSCALAEHKPYSIGDSYISELAT
RENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
PTTTTLAWKKARLINCTFNEPPLVDAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAW
EMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR

Sequences:

>Translated_378_residues
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSCALAEHKPYSIGDSYISELAT
RENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
PTTTTLAWKKARLINCTFNEPPLVDAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAW
EMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR
>Mature_377_residues
SHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSCALAEHKPYSIGDSYISELATR
ENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLGP
TTTTLAWKKARLINCTFNEPPLVDAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNGV
IGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWE
MFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA [H]

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family [H]

Homologues:

Organism=Escherichia coli, GI87082206, Length=378, Percent_Identity=99.2063492063492, Blast_Score=779, Evalue=0.0,
Organism=Escherichia coli, GI1790830, Length=192, Percent_Identity=32.8125, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.174 [H]

Molecular weight: Translated: 42339; Mature: 42208

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSC
CCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHH
ALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLAL
HHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
LEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLGPTTTTLAWKKARLINCTFNE
HHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCC
PPLVDAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
CCCCCCCCCEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRCEFMINNALSGVEPFRFN
EEEEEEECCCCCEEEEEECCCCHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCCCCEEEE
AVLCNPPFHQQHALTDNVAWEMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHHCCCEEE
ATNNKFVVLKAVKLGRRR
EECCCEEEEEEECCCCCC
>Mature Secondary Structure 
SHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSC
CCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHH
ALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLAL
HHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
LEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLGPTTTTLAWKKARLINCTFNE
HHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCC
PPLVDAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
CCCCCCCCCEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRCEFMINNALSGVEPFRFN
EEEEEEECCCCCEEEEEECCCCHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCCCCEEEE
AVLCNPPFHQQHALTDNVAWEMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHHCCCEEE
ATNNKFVVLKAVKLGRRR
EECCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA