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The map label for this gene is insK [C]

Identifier: 170684064

GI number: 170684064

Start: 2279746

End: 2280636

Strand: Reverse

Name: insK [C]

Synonym: EcSMS35_2258

Alternate gene names: 170684064

Gene position: 2280636-2279746 (Counterclockwise)

Preceding gene: 170682322

Following gene: 170682772

Centisome position: 45.0

GC content: 53.87

Gene sequence:

>891_bases
ATGATGCCACTGCTGGATAAGCTGCGTGAGCAGTACGGGGTCGGACCGGTATGCAGCGAACTGCATATTGCCCCGTCAAC
GTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAG
AGATACTGCGCGTATACGATGAAAATCATCAGGTGTACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATC
AGGGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAC
GACCATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGTCCTGACCAGCTGTGGG
TAGCTGATTTTACTTACGTCAGCACATGGCAGGGCTTCGTCTATGTGGCGTTCATCATTGATGTATTTGCCGGATACATC
GTGGGGTGGCGAGTCTCATCGTCTATGGAAACGACATTCGTGCTGGATGCACTGGAGCAGGCGTTGTGGGCCCGCCGGCC
GTCCGGCACAATCCACCACAGTGATAAAGGTTCTCAGTATGTATCGCTGGCCTACACGCAGCGGCTTAAGGAAGCCGGCT
TACTGGCCTCAACAGGAAGTACTGGTGACTCGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAG
GTAATACACCGTAAGAGTTGGAAAAACCGGACAGAAGTGGAACTGGCCACACTAACGTGGGTGGACTGGTATAACAATCG
ACGATTGCTGGAAAGGCTGGGCCATATTCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAATACTGATC
TGGCAGCCTGA

Upstream 100 bases:

>100_bases
AGCTGGAGCGTGAAAATCGTGAACTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGTGGAG
TTCGACCGCCTCTGGAAAAA

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAACCCGGGGCGGTTCAGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGC
GGCACAGTTGGTGTGGCGGC

Product: IS629 transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYDENHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNTDLAA

Sequences:

>Translated_296_residues
MMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYDENHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNTDLAA
>Mature_296_residues
MMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYDENHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNTDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=244, Percent_Identity=52.8688524590164, Blast_Score=252, Evalue=4e-67,
Organism=Escherichia coli, GI1789981, Length=286, Percent_Identity=29.3706293706294, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788405, Length=292, Percent_Identity=28.4246575342466, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787263, Length=292, Percent_Identity=28.4246575342466, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786753, Length=292, Percent_Identity=28.4246575342466, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786570, Length=292, Percent_Identity=28.4246575342466, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786490, Length=292, Percent_Identity=28.4246575342466, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33794; Mature: 33794

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYD
CCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
ENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQ
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
ALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
HHHHCCCCCCEEECCCCCCEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
VIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNTDLAA
HHHHHHCCCCCCEEEEEEEEEHHHCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYD
CCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
ENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQ
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
ALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
HHHHCCCCCCEEECCCCCCEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
VIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNTDLAA
HHHHHHCCCCCCEEEEEEEEEHHHCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]