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The map label for this gene is yfeR [H]

Identifier: 170684030

GI number: 170684030

Start: 2616908

End: 2617834

Strand: Reverse

Name: yfeR [H]

Synonym: EcSMS35_2563

Alternate gene names: 170684030

Gene position: 2617834-2616908 (Counterclockwise)

Preceding gene: 170681427

Following gene: 170683003

Centisome position: 51.65

GC content: 54.8

Gene sequence:

>927_bases
ATGAATTATTCTTTAAAACAATTAAAGGTTTTCGTCACAGTAGCGCAGGAGAAAAGTTTTAGTCGTGCAGGAGAGCGTAT
CGGCCTGAGCCAGTCGGCAGTGAGTCACAGTGTGAAGGAGCTGGAAAATCATACTGGTGTTCGCCTGCTGGACAGAACCA
CGCGTGAAGTGGTGCTTACAGATGCAGGGCAGCAGTTGGCTTTGCGTCTTGAGCGACTGCTGGATGAACTTAACAGCACG
TTGCGCGATACCGGGCGTATGGGGCAACAACTGAGCGGAAAAGTTCGGGTCGCTGCCAGCCAGACCATTTCCGCGCATCT
TATTCCGCAATGCATTGCTGAAAGCCATCGCCGCTATCCAGATATTCAGTTTGTCCTGCACGATCGTCCGCAGCAGTGGG
TGATGGAAAGTATTCGCCAGGGAGATGTCGATTTTGGCATCGTCATCGATCCAGGCCCAGTGAGCGATCTGCAATGTGAA
GCGATTCTTTCCGAGCCTTTCTTCTTGCTTTGCCATCGCGATAGCGCTTTGGCCGCGGAAGATTATGTCCCCTGGCAGGC
ATTACAGGGAGCGAAATTGGTGTTGCAGGATTACGCGTCAGGCAGCCGACCGCTGATTGACGCAGCTCTGGCGCGCAATG
GTATTCAGGCGAATATTGTGCAGGAGATTGGACATCCGGCGACGCTGTTCCCGATGGTAGCCGCAGGCATCGGCATCAGT
ATTCTTCCCGCACTGGCGCTACCATTGCCTGAAGGTAGCCCGCTGGTGGTAAAGCGCATTACGCCGGTGGTGGAACGCCA
GTTGATGCTGGTGCGCAGGAAGAACCGTTCACTTTCCACCGCCGCACAAGCGTTATGGGACGTGGTACGAGGCCAGGGCC
GTGCGCTAATGGCTGGGCGGGAAGGGGATCCGCTGTATCAGATATAG

Upstream 100 bases:

>100_bases
AAAAGTTTCATAAAGACTCCAGATGATCGATGGTTCTATTGTGCTTCTTCCAGTTTGAGAAATAAAATTGATTTATTGCA
TCCATTCATGAAGAAAACAG

Downstream 100 bases:

>100_bases
AGCTATTCATACTGCCGGGAGCCCCGGCAGTAATCAATCAGAAATTAAATTTGATGGAGTAGATTATTGCATCCTGGTAA
AAATCTTCGCCCAGTTTGTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRLERLLDELNST
LRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAQALWDVVRGQGRALMAGREGDPLYQI

Sequences:

>Translated_308_residues
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRLERLLDELNST
LRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAQALWDVVRGQGRALMAGREGDPLYQI
>Mature_308_residues
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRLERLLDELNST
LRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAQALWDVVRGQGRALMAGREGDPLYQI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788748, Length=308, Percent_Identity=98.3766233766234, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI145693105, Length=287, Percent_Identity=33.1010452961672, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI157672245, Length=280, Percent_Identity=27.8571428571429, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790399, Length=292, Percent_Identity=26.7123287671233, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=29.1497975708502, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=28.0821917808219, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=29.9180327868852, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788297, Length=244, Percent_Identity=28.2786885245902, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI2367136, Length=239, Percent_Identity=25.9414225941423, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787601, Length=169, Percent_Identity=28.9940828402367, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787128, Length=123, Percent_Identity=34.9593495934959, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081904, Length=179, Percent_Identity=29.608938547486, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786401, Length=126, Percent_Identity=34.1269841269841, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788706, Length=169, Percent_Identity=27.810650887574, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789506, Length=291, Percent_Identity=23.7113402061856, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787589, Length=122, Percent_Identity=32.7868852459016, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=246, Percent_Identity=22.3577235772358, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788296, Length=251, Percent_Identity=27.0916334661355, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789173, Length=167, Percent_Identity=27.5449101796407, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790208, Length=299, Percent_Identity=24.7491638795987, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786713, Length=123, Percent_Identity=31.7073170731707, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787530, Length=264, Percent_Identity=22.3484848484848, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786508, Length=99, Percent_Identity=31.3131313131313, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=162, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=118, Percent_Identity=28.8135593220339, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789440, Length=168, Percent_Identity=27.9761904761905, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33889; Mature: 33889

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLT
CCCCHHHHEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEE
DAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
DIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCEAILSEPFFLLCHRDSALAAE
CEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEECCCCCCHH
DYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
CCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHH
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAQALWDVVRGQGRALMAGR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECC
EGDPLYQI
CCCCCCCC
>Mature Secondary Structure
MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRTTREVVLT
CCCCHHHHEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEE
DAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
DIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCEAILSEPFFLLCHRDSALAAE
CEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEECCCCCCHH
DYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGIS
CCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHH
ILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAQALWDVVRGQGRALMAGR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECC
EGDPLYQI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]