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The map label for this gene is rihA [H]

Identifier: 170684012

GI number: 170684012

Start: 684133

End: 685068

Strand: Reverse

Name: rihA [H]

Synonym: EcSMS35_0672

Alternate gene names: 170684012

Gene position: 685068-684133 (Counterclockwise)

Preceding gene: 170680355

Following gene: 170683933

Centisome position: 13.52

GC content: 50.53

Gene sequence:

>936_bases
ATGGCACTGCCAATTCTGTTAGATTGCGACCCAGGTCATGACGACGCTATCGCAATCGTTCTCGCCCTCGCCTCACCAGA
GCTTGATGTCAAAGCAATTACGTCTTCCGCCGGAAACCAGACACCAGAAAAAACCTTACGCAATGTTCTGTGTATGCTGA
CCTTGCTTAATCGCACCGATATTCCGGTAGCAAGCGGTGCGGTAAAACCATTAATGCGTGATTTGATTATCGCGGACAAT
GTGCACGGCGAAAGCGGTCTCGACGGCCCGGCATTACCGGAACCGACGTTCGCACCGCAAAACTGTACGGCGGTGGAGCT
GATGGCGAAAACGCTGTGTGAAAGTGAGGAACCTGTCACCATTGTTTCTACCGGACCGCAAACTAACGTTGCCTTGCTGC
TCAATAGCCACCCGGAACTGCATAGCAAAATTGCCCGTATCGTGATTATGGGCGGCGCAATGGGGCTTGGTAACTGGACG
CCTGCGGCTGAATTTAATATTTACGTTGACCCGGAAGCGGCAGAAATTGTCTTCCAGTCAGGGATCCCGGTGGTGATGGC
CGGTCTGGATGTTACTCACAAAGCACAAATTCACGTTGAGGACACCGAGCGTTTCCGCGCCATTGGCAACCCTGTTTCAA
CCATTGTTGCCGAACTGCTGGATTTCTTCCTCGAATATCATAAAGACGAAAAATGGGGCTTTGTCGGCGCACCGCTGCAT
GACCCATGCACCATCGCCTGGCTGTTAAAACCGGAGTTGTTTACCACTGTTGAGCGCTGGGTTGGCGTGGAAACACAAGG
GAAATATACCCAAGGTATGACGGTTGTTGATTATTATTATCTGACTGGCAATAAACCGAATGCCACTGTAATGGTCGATG
TTGATCGTCAGGGCTTTGTTGATTTACTGGCCGATCGTCTGAAATTTTACGCTTAA

Upstream 100 bases:

>100_bases
CCCAAGTGCGCGCCATCTCACGCTTTATCGTTTCGCTCCTCGTATCTGTTACATCCTGGCGGTACCCTTGTCGCAAAGAA
GCACACAACAAGGAGCAACA

Downstream 100 bases:

>100_bases
CACACGTCATTGATTAAGATCACCACGCGTTACCTGGGTGGTCTTAATCTGTTTATCTCTTTTTCTTTCAAGATATAAAT
GCAATGGATAATGCAGAACT

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLCMLTLLNRTDIPVASGAVKPLMRDLIIADN
VHGESGLDGPALPEPTFAPQNCTAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLH
DPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA

Sequences:

>Translated_311_residues
MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLCMLTLLNRTDIPVASGAVKPLMRDLIIADN
VHGESGLDGPALPEPTFAPQNCTAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLH
DPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA
>Mature_310_residues
ALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLCMLTLLNRTDIPVASGAVKPLMRDLIIADNV
HGESGLDGPALPEPTFAPQNCTAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWTP
AAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLHD
PCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=311, Percent_Identity=98.0707395498392, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1788486, Length=306, Percent_Identity=40.5228758169935, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1786213, Length=309, Percent_Identity=44.0129449838188, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=30.1587301587302, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=32.4468085106383, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=37.0656370656371, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24583915, Length=326, Percent_Identity=26.6871165644172, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641839, Length=308, Percent_Identity=28.8961038961039, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641837, Length=324, Percent_Identity=28.3950617283951, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33805; Mature: 33674

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLCMLTLLNRTD
CCCEEEEECCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
IPVASGAVKPLMRDLIIADNVHGESGLDGPALPEPTFAPQNCTAVELMAKTLCESEEPVT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
IVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWTPAAEFNIYVDPEAAEIVFQS
EEECCCCCEEEEEECCCHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEECCCHHEEEECC
GIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLH
CCCEEEECCCCCEEEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
DPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFV
CCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHH
DLLADRLKFYA
HHHHHHHHHCC
>Mature Secondary Structure 
ALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLCMLTLLNRTD
CCEEEEECCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
IPVASGAVKPLMRDLIIADNVHGESGLDGPALPEPTFAPQNCTAVELMAKTLCESEEPVT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
IVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWTPAAEFNIYVDPEAAEIVFQS
EEECCCCCEEEEEECCCHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEECCCHHEEEECC
GIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLH
CCCEEEECCCCCEEEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
DPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFV
CCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHH
DLLADRLKFYA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA