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The map label for this gene is ybiY [H]

Identifier: 170683998

GI number: 170683998

Start: 854291

End: 855190

Strand: Reverse

Name: ybiY [H]

Synonym: EcSMS35_0849

Alternate gene names: 170683998

Gene position: 855190-854291 (Counterclockwise)

Preceding gene: 170680893

Following gene: 170680406

Centisome position: 16.87

GC content: 54.22

Gene sequence:

>900_bases
ATGATTTTCAATATTCAGCGCTACTCGACCCATGATGGCCCCGGTATCCGCACGGTCGTATTTCTTAAAGGCTGTTCGCT
GGGCTGCCGCTGGTGTCAGAACCCGGAAAGCCGAGCCCGCACGCAGGATCTGTTGTATGACGCACGACTGTGTCTGGAAG
GCTGCGAGCTGTGCGCTAAGGCCGCGCCGGACGTGATTGAGCGCTCGCTGAATGGTTTGCTTATTCATCGGGAAAAGTTA
ACCCCGGAGCATCTGACAGCGTTAACCGACTGCTGTCCGACACAGGCGTTAACCGTGTGTGGTGAAGTGAAAAGCGTTGA
GGAGATCATGGCGACCGTTTTGCGCGATAAACCGTTTTACGATCGCAGCGGCGGCGGCTTAACACTTTCTGGCGGTGAAC
CCTTTATGCAGCCGGAAATGGCGACGGAGCTACTGCAAGCCAGCCACGAGGCCGGCATTCATACTGCGGTAGAAACCTGT
CTGCATGTGCCGTGGAAATATATCTCCCCTTCTCTGCCCTATATCGATCTGTTTCTTGCCGATTTAAAACACGTCGCCGA
CGCGCCGTTTAAACAGTGGACCGACGGTAACGCCGCCAGGGTGCTGGATAATCTGAAAAAACTCGCTGCGGCGGGGAAAA
AAATCATCATCCGCGTACCGCTGATTCAGGGCTTTAATGCCGACGAAACCGCTGTAAAAGCCATTACCGATTTTGCCGCC
GACGAGCTGCACGTTGGCGAAATCCATTTCCTGCCCTACCACACGCTGGGCATCAACAAATATCACTTACTTAATCTGCC
CTATGACGCCCCGGAAAAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCCAGCAGTATGCCTGCCAGAAAGGGTTAA
CCGCGACCTTACGAGGATAA

Upstream 100 bases:

>100_bases
ATGTAGACTATCACGGGAAAACTCTCTGAACAGTTGACACACATCAATCTAACTTTCATTCGAAAGTATTTTAATGTTTG
AACGAAACAGAGAGGCAACT

Downstream 100 bases:

>100_bases
CAACCATGACCACACTGAAACTGGACACGCTCAGCGACCGCATTAAAGCGCACAAAAATGCGCTGGTGCATATTGTGAAA
CCACCAGTCTGTACCGAGCG

Product: glycyl-radical activating family protein

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPDVIERSLNGLLIHREKL
TPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATELLQASHEAGIHTAVETC
LHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETAVKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Sequences:

>Translated_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPDVIERSLNGLLIHREKL
TPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATELLQASHEAGIHTAVETC
LHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETAVKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
>Mature_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPDVIERSLNGLLIHREKL
TPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATELLQASHEAGIHTAVETC
LHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETAVKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=97.6588628762542, Blast_Score=585, Evalue=1e-169,
Organism=Escherichia coli, GI1790389, Length=301, Percent_Identity=36.2126245847176, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787130, Length=171, Percent_Identity=32.1637426900585, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 33038; Mature: 33038

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAK
CEEECEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAPDVIERSLNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFY
CCHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRSGGGLTLSGGEPFMQPEMATELLQASHEAGIHTAVETCLHVPWKYISPSLPYIDLFLA
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCHHHHHHH
DLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETAVKAITDFAA
HHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHC
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
CCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCC
>Mature Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAK
CEEECEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAPDVIERSLNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFY
CCHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRSGGGLTLSGGEPFMQPEMATELLQASHEAGIHTAVETCLHVPWKYISPSLPYIDLFLA
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCHHHHHHH
DLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETAVKAITDFAA
HHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHC
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
CCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]