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The map label for this gene is cynR [H]

Identifier: 170683944

GI number: 170683944

Start: 382023

End: 382922

Strand: Reverse

Name: cynR [H]

Synonym: EcSMS35_0369

Alternate gene names: 170683944

Gene position: 382922-382023 (Counterclockwise)

Preceding gene: 170682300

Following gene: 170684111

Centisome position: 7.56

GC content: 55.11

Gene sequence:

>900_bases
ATGCTCTCTCGACATATCAATTATTTTCTTGCCGTGGCTGAACATGGCAGCTTTACCCGTGCCGCCAGTGCGTTGCACGT
CTCCCAGCCTGCGCTTTCCCAGCAGATTCGCCAGTTAGAGGAGAGTTTAGGCGTGCCGCTGTTTGACCGTAGCGGGCGAA
CGATTCGTCTCACTGATGCGGGAGAAGTCTGGCGACAGTACGCCAGCCGGGCGTTACAGGAACTGGGGGCGGGTAAACGG
GCGATTCATGATGTTGCCGATCTGACGCGAGGATCGCTGCGTATCGCCGTCACTCCCACCTTTACAAGCTACTTTATCGG
CCCTTTAATGGCGGATTTCTATGCGCGCTACCCCGGCATCACGCTCCAGCTACAGGAAATGTCGCAGGAGAAAATCGAAG
ATCTGCTTTGTCGCGACGAGCTGGACGTTGGGATTGCCTTCGCGCCTGCGCATTCGCCGGAGCTGGAGGCAATTCCTTTA
CTGACAGAAAGTTTAGCGTTAGTCGTGGCGCAACATCATCCGCTGGCTGCCTGTGAACAGGTGGCGTTGAGTCGCTTGCA
TGATGAAAAACTGGTCCTGCTCAGCGCGGAATTTGCCACCAGAGAGCAAATTGACCACTACTGCGAGAAAGCGGGGCTAC
ATCCACAGGTGGTCATTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTTCCACATTGTTG
CCAGCAGCGATTGCCACACAACATGACGGGCTTAAAGCGATTTCTCTTGCCCCACCGTTACTGGAGAGAACGGCGGTTTT
GTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGCGAAGGCATTTTTACACATGGCGTTGGATAAATGCGCGGTTGTTG
GCGGAAATGAATCGCGGTAA

Upstream 100 bases:

>100_bases
CTGTCTGTCTCTGGAATGAGAGGCAGACTACTCGCCGATTGTCATAAGGTAAAAGTCTCATTTATGATGAGTTCTATTGG
ATTTACTTATAGGTTGGCGA

Downstream 100 bases:

>100_bases
CCACTGACACAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGTTCAGTGCTCTACGTAGGCCGGATAAGGCGTTCA
CGCCGCATCCGGCATAAACT

Product: DNA-binding transcriptional regulator CynR

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPAHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALDKCAVVGGNESR

Sequences:

>Translated_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPAHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALDKCAVVGGNESR
>Mature_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPAHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALDKCAVVGGNESR

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=299, Percent_Identity=98.3277591973244, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1790399, Length=276, Percent_Identity=30.7971014492754, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=28.7671232876712, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI145693105, Length=284, Percent_Identity=27.8169014084507, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788748, Length=300, Percent_Identity=27.3333333333333, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787530, Length=235, Percent_Identity=29.7872340425532, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081904, Length=225, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367136, Length=227, Percent_Identity=31.2775330396476, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=30.6451612903226, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787601, Length=294, Percent_Identity=24.4897959183673, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=30.1369863013699, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789173, Length=212, Percent_Identity=31.6037735849057, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786401, Length=171, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789204, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790208, Length=182, Percent_Identity=31.3186813186813, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788296, Length=213, Percent_Identity=28.6384976525822, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790262, Length=236, Percent_Identity=25, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=24.5614035087719, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787949, Length=168, Percent_Identity=32.7380952380952, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1789639, Length=142, Percent_Identity=33.8028169014084, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788481, Length=206, Percent_Identity=29.6116504854369, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786448, Length=158, Percent_Identity=34.1772151898734, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=160, Percent_Identity=30.625, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787589, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=173, Percent_Identity=32.9479768786127, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=222, Percent_Identity=27.4774774774775, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786713, Length=175, Percent_Identity=29.7142857142857, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87082184, Length=252, Percent_Identity=25.3968253968254, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081684, Length=294, Percent_Identity=22.7891156462585, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32823; Mature: 32823

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLQLQEMSQEKIEDLLCRDELDVGIAFAPAHSPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALDKCAVVGGNESR
HHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLQLQEMSQEKIEDLLCRDELDVGIAFAPAHSPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALDKCAVVGGNESR
HHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]