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The map label for this gene is yebU [H]

Identifier: 170683942

GI number: 170683942

Start: 1338431

End: 1339876

Strand: Reverse

Name: yebU [H]

Synonym: EcSMS35_1352

Alternate gene names: 170683942

Gene position: 1339876-1338431 (Counterclockwise)

Preceding gene: 170684215

Following gene: 170682835

Centisome position: 26.44

GC content: 52.77

Gene sequence:

>1446_bases
ATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCTATGCCTTCGACGCTCTC
TTTTGATGATTTTCTTGCCGCCTGTCAGCGCCCGTTGCGCCGCAGCATTCGCGTTAATACGCTGAAAATCTCCGTTGCTG
ATTTCCTGCAATTAACCGCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGC
GACGATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGCGGCCTGTTTTATATTCAGGAAGCCAGTTCAAT
GTTGCCCGTTGCCGCCTTGTTTGCTGACGGTAATGCACCACAGCGGGTGATGGATGTCGCTGCCGCGCCCGGCTCCAAAA
CGACGCAAATTGCCGCGCGGATGAATAACGAAGGGGCGATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTA
CATGCCAATATCAGCCGCTGTGGCATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCC
AGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCCGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAA
ACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCGACCCAACGGGAACTGATCGACAGCGCCTTTCATGCATTACGCCCT
GGCGGTACGCTGGTTTACTCGACCTGTACCTTAAACAGGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTA
CCCCGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGCAAATAAGGCGCTGACCGAAGAAGGCTTTTTGC
ATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATCCCCGTCTTACCC
GCCCCAAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGC
AGGTGTTGGCTTAAACTGGGATGGAAACCTGCGACTCTGGCAACGCGACAAAGAACTGTGGTTGTTCCCGGTAGGCATTG
AAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAAACGCATAACAAAGGTTATCGCTGGCAG
CATGAAGCGGTTATTGCCCTTGCCTCCCCCGACAATGTGAACGCTTTTGAACTGACACCGCAGGAAGCGGAAGAGTGGTA
TCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTAGCGGATGATGTATTGGTTACTTTCCAGCATCAGCCGATTGGTT
TAGCCAAACGAATTGGTTCGCGACTGAAAAACAGCTACCCGCGTGAACTGGTGCGAGACGGGAAACTTTTTACCGGTAAC
GCCTGA

Upstream 100 bases:

>100_bases
GAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGTCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCC
TGTTTTTTTGCCAGTGGTAT

Downstream 100 bases:

>100_bases
CAGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGG
TCGTACCACAATCGGCAGCT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIER
DDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
GGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLP
APKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
A

Sequences:

>Translated_481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIER
DDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
GGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLP
APKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
A
>Mature_481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIER
DDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
GGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLP
APKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
A

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=306, Percent_Identity=37.2549019607843, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI76150623, Length=306, Percent_Identity=37.2549019607843, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI11545785, Length=167, Percent_Identity=38.3233532934132, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI32698918, Length=160, Percent_Identity=35, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI301336155, Length=286, Percent_Identity=28.3216783216783, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI39995082, Length=286, Percent_Identity=28.3216783216783, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI23199998, Length=356, Percent_Identity=28.6516853932584, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI270288816, Length=332, Percent_Identity=29.5180722891566, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI270288818, Length=321, Percent_Identity=29.2834890965732, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI8922322, Length=321, Percent_Identity=29.2834890965732, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40316918, Length=210, Percent_Identity=30.4761904761905, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=98.3298538622129, Blast_Score=977, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=220, Percent_Identity=32.2727272727273, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=320, Percent_Identity=33.4375, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI25143471, Length=193, Percent_Identity=32.1243523316062, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=322, Percent_Identity=27.0186335403727, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=229, Percent_Identity=29.2576419213974, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=296, Percent_Identity=36.8243243243243, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=32.0388349514563, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI22024126, Length=334, Percent_Identity=34.7305389221557, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355201, Length=207, Percent_Identity=33.8164251207729, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=35.4285714285714, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356513, Length=175, Percent_Identity=35.4285714285714, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53347; Mature: 53347

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTA
CEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHC
PYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAP
CCCCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHCEEEEEECCCCCCCEEEEEECCCCH
QRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGR
HHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCE
VFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
EHHHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
GGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIY
CCEEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCCHHCCCCCEEECCEEE
DCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLW
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
QRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTP
EECCEEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECH
QEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
HHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECC
A
C
>Mature Secondary Structure
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTA
CEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHC
PYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAP
CCCCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHCEEEEEECCCCCCCEEEEEECCCCH
QRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGR
HHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCE
VFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
EHHHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
GGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIY
CCEEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCCHHCCCCCEEECCEEE
DCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDGNLRLW
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
QRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTP
EECCEEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECH
QEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
HHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA