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The map label for this gene is aegA2 [H]

Identifier: 170683856

GI number: 170683856

Start: 3107813

End: 3109747

Strand: Reverse

Name: aegA2 [H]

Synonym: EcSMS35_3019

Alternate gene names: 170683856

Gene position: 3109747-3107813 (Counterclockwise)

Preceding gene: 170682698

Following gene: 170680835

Centisome position: 61.36

GC content: 53.13

Gene sequence:

>1935_bases
ATGAAAGGGATGCAAATGAATAAGTTTATCGCTGCTGAAGCTGCGGAATGTATAGGCTGTCATGCTTGTGAAATTGCCTG
TGCGATGGCACATAATCAAGAAAACTGGCCGCTGAGTCACAGTGACTTTCGACCGCGTATCCACGTTGTAGGGAAAGGCC
AGGCTGCGAATCCGGTGGCCTGCCATCACTGCAACAATGCCCCTTGCGTTACGGCTTGTCCGGTTAATGCTCTGACTTTC
CAGCCCGATAGCGTACAACTGGACGAGCAAAAATGTATTGGTTGTAAAAGATGCGCAATCGCTTGCTCCTTTGGCGTCGT
TGAGATGGTCGATACGATTGCGCAGAAATGCGACCTTTGTAACCAGCGCAGTTCCGGCACGCAAGCCTGTATTGAAGTCT
GCCCAACACAGGCGTTACGGCTGATGGACGATAAAGGGTTGCAGCAGATAAAGGTGGCCCGCCAGCGCAAAACGGCAGCA
GGAAAAGCGTCATCAGACGCTCAGCCATCTCGCAGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAGATAACAT
TTCAGCGAGTGAACGGAAAACCCACTTTGGCGAAATCTATTACGGGCTGGATCCACAACAAGCGACTTATGAGAGTGACC
GCTGTGTTTATTGCGCCGAAAAAGCTAACTGCAACTGGCATTGCCCGCTGCATAACGCTATTCCGGATTACATCCGTCTG
GTGCAGGAAGGAAAGATTATTGAAGCGGCAGAACTTTGCCACCAGACCAGTTCCTTACCAGAAATCTGCGGCAGGGTATG
TCCACAGGACCGTCTTTGTGAAGGCGCATGTACTTTGAAAGATCACTCTGGCGCAGTCTCTATCGGGAATCTGGAACGCT
ACATCACCGATACCGCGCTGGCGATGGGCTGGCGTCCCGATGTCAGCAAAGTTGTTCCCCGTATCGAAAAAGTGGCGGTG
ATTGGCGCAGGGCCTGCAGGGTTAGGGTGTGCTGATATTCTGGCGCGCGCAGGAGTTCAGGTTGATGTCTTTGATCGCCA
TCCAGAAATTGGCGGTATGCTGACTTTTGGCATACCTCCTTTCAAACTCGATAAAACGGTATTAAGCCAGCGGCGAGAGA
TATTCACCGCAATGGGAATCGACTTTCATCTTAACTGTGAAATTGGCCGCGATATTACCTTTAGCGATTTAACTTCTGAA
TATGATGCAGTTTTCATCGGCGTGGGGACTTACGGGATGATGCGAGCAGATCTGCCGCATGAAGATGCGCCCGGTGTCAT
TCAGGCTCTACCGTTCCTGACTGCCCATACCCGCCAGCTCATGGGGTTGCCGGAGTCTGAAGAGTATCCGCTGACGGATG
TGGAAGGTAAGCGAGTCGTGGTATTGGGCGGTGGCGATACGACCATGGATTGTTTGCGGACCTCCATCCGCCTCAATGCC
GCCAGCGTGACCTGCGCGTATCGTCGTGATGAAGTCAGTATGCCGGGCTCGCGCAAAGAGGTGGTCAATGCGCGCGAGGA
AGGTGTCGAGTTTCAGTTCAATGTTCAGCCGCAATATATCGCTTGTGACGAAGATGGGCGTTTAACTGCAGTGGGCCTGA
TTCGTACCGCCATGGGGGAGCCGGGGCCGGATGGTCGCCGTCGTCCTCGTCCGGTAGCGGGTTCAGAGTTTGAATTGCCC
GCCGATGTTCTCATTATGGCCTTTGGTTTCCAGGCGCATGCCATGCCGTGGTTGCAGGGCAGCGGAATTAAACTCGATAA
ATGGGGCCTGATTCAAACCGGTGACGTCGGGTATTTACCTACCCAGACGCATCTGAAAAAAGTCTTTGCTGGTGGTGATG
CAGTTCATGGCGCGGATCTGGTTGTCACTGCAATGGCCGCAGGAAGGCAGGCGGCGCGCGATATGTTAACTCTGTTTGAT
ACGAAGGCATCGTGA

Upstream 100 bases:

>100_bases
TAATTTGAACTCAGTCGCAAAATTATAATTAAATCGGCCATGCTACCTAATATATGCCCATTTATTGCATCGCGAAATTT
GAGAAGACATAACTCATTAA

Downstream 100 bases:

>100_bases
TGAAATCGTTAATTATCGTTAATCCGGCTGACTGTATTGGCTGCCGTACTTGTGAAGTAGCCTGTGTGGTCGCTCACCCT
TCAGAACAGGAGTTGAATGC

Product: putative oxidoreductase Fe-S binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MKGMQMNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCNNAPCVTACPVNALTF
QPDSVQLDEQKCIGCKRCAIACSFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAA
GKASSDAQPSRSAALLPVNSRKGADNISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRL
VQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAV
IGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSE
YDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNA
ASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELP
ADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFD
TKAS

Sequences:

>Translated_644_residues
MKGMQMNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCNNAPCVTACPVNALTF
QPDSVQLDEQKCIGCKRCAIACSFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAA
GKASSDAQPSRSAALLPVNSRKGADNISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRL
VQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAV
IGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSE
YDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNA
ASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELP
ADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFD
TKAS
>Mature_644_residues
MKGMQMNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCNNAPCVTACPVNALTF
QPDSVQLDEQKCIGCKRCAIACSFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAA
GKASSDAQPSRSAALLPVNSRKGADNISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRL
VQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAV
IGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSE
YDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNA
ASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELP
ADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFD
TKAS

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=472, Percent_Identity=29.4491525423729, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI111118983, Length=271, Percent_Identity=25.830258302583, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI111118981, Length=162, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=639, Percent_Identity=99.0610328638498, Blast_Score=1309, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=656, Percent_Identity=54.4207317073171, Blast_Score=728, Evalue=0.0,
Organism=Escherichia coli, GI1789606, Length=450, Percent_Identity=59.5555555555556, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=33.6448598130841, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=28.7305122494432, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082179, Length=163, Percent_Identity=44.7852760736196, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI2367245, Length=156, Percent_Identity=43.5897435897436, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=38.150289017341, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789079, Length=189, Percent_Identity=33.8624338624339, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082114, Length=203, Percent_Identity=33.0049261083744, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI2367345, Length=164, Percent_Identity=31.0975609756098, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789370, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787122, Length=161, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17570289, Length=470, Percent_Identity=30.4255319148936, Blast_Score=179, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71984108, Length=521, Percent_Identity=29.5585412667946, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17543860, Length=254, Percent_Identity=28.740157480315, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320030, Length=475, Percent_Identity=31.1578947368421, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=33.1958762886598, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=33.1958762886598, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=33.2644628099174, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=33.2644628099174, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24640763, Length=457, Percent_Identity=28.0087527352298, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI18858217, Length=457, Percent_Identity=28.0087527352298, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00037 Fer4; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 69770; Mature: 69770

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMQMNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHVVGKGQAANPVA
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEE
CHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACSFGVVEMVDTIAQKCDLC
EECCCCCCEEEECCCCEEEECCCCCEECHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHH
NQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASSDAQPSRSAALLPVNS
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
RKGADNISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRL
CCCCCCCCCCHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHH
VQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTAL
HHCCCEEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCEEECCCCEEEECCHHHHHHHHHH
AMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPP
HCCCCCCHHHHHCHHHEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCC
FKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRADLPH
CHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCEEEEEECCCCEEECCCCC
EDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEEC
ASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGE
EEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHCCC
PGPDGRRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLP
CCCCCCCCCCCCCCCCCCCCHHHEEEEECCHHHCCCCCCCCCEEEECCCCEEECCCCCCC
TQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGMQMNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHVVGKGQAANPVA
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEE
CHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACSFGVVEMVDTIAQKCDLC
EECCCCCCEEEECCCCEEEECCCCCEECHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHH
NQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASSDAQPSRSAALLPVNS
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
RKGADNISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRL
CCCCCCCCCCHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHH
VQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTAL
HHCCCEEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCEEECCCCEEEECCHHHHHHHHHH
AMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPP
HCCCCCCHHHHHCHHHEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCC
FKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRADLPH
CHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCEEEEEECCCCEEECCCCC
EDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEEC
ASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGE
EEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHCCC
PGPDGRRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLP
CCCCCCCCCCCCCCCCCCCCHHHEEEEECCHHHCCCCCCCCCEEEECCCCEEECCCCCCC
TQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]