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The map label for this gene is manY

Identifier: 170683840

GI number: 170683840

Start: 1356211

End: 1357011

Strand: Reverse

Name: manY

Synonym: EcSMS35_1370

Alternate gene names: 170683840

Gene position: 1357011-1356211 (Counterclockwise)

Preceding gene: 170680281

Following gene: 170680039

Centisome position: 26.77

GC content: 51.56

Gene sequence:

>801_bases
ATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATT
TCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTATCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTG
GTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCCATCATT
TCTACTATTCTGGTTATCGCAGGTCATCAGAGCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCA
GGTACTGACCATCATCGTTCGTACTATCACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGA
CAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTG
TCTGTTGGTACCAGCGAAGTCCAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCAGGTGG
CATGATCGTAGTGGTTGGTTATGCGATGGTTATCAACATGATGCGTGCTGGCTACCTGATGCCGTTCTTCTACCTCGGCT
TCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGCTCTACATCCAA
CTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAGCTGGTAACAACGATCTCGATAACGAACTGGACTA
A

Upstream 100 bases:

>100_bases
TGAAAATGATGGATCTGATCAGCAAAATCGATAAGTAACGTATTGTGTTGATTATCACTCAGTTTTCACACTTAAGTCTT
ACGTAAACAGGAGAAGTACA

Downstream 100 bases:

>100_bases
CAGGTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCT
GCGTTCTAACCTCTTCCAGG

Product: PTS system, mannose-specific IIC component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-P-Man; EIIC-Man; PTS system mannose-specific EIIC component

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLGDMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASII
STILVIAGHQSIGAGIALAIPLAAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAAFTNFNLVALGVIGTVMAVLYIQ
LSPKYNRVAGAPAQAAGNNDLDNELD

Sequences:

>Translated_266_residues
MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLGDMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASII
STILVIAGHQSIGAGIALAIPLAAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAAFTNFNLVALGVIGTVMAVLYIQ
LSPKYNRVAGAPAQAAGNNDLDNELD
>Mature_266_residues
MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLGDMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASII
STILVIAGHQSIGAGIALAIPLAAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAAFTNFNLVALGVIGTVMAVLYIQ
LSPKYNRVAGAPAQAAGNNDLDNELD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3715

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-4 domain

Homologues:

Organism=Escherichia coli, GI1788121, Length=266, Percent_Identity=100, Blast_Score=522, Evalue=1e-150,
Organism=Escherichia coli, GI1789528, Length=263, Percent_Identity=25.8555133079848, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTNC_ECO57 (P69803)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85793
- PIR:   H90944
- RefSeq:   NP_288254.1
- RefSeq:   NP_310555.1
- ProteinModelPortal:   P69803
- EnsemblBacteria:   EBESCT00000026064
- EnsemblBacteria:   EBESCT00000059900
- GeneID:   912707
- GeneID:   961791
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2861
- KEGG:   ecs:ECs2528
- GeneTree:   EBGT00050000009642
- HOGENOM:   HBG417063
- OMA:   LSPKYNK
- ProtClustDB:   PRK15065
- BioCyc:   ECOL83334:ECS2528-MONOMER
- InterPro:   IPR004700
- InterPro:   IPR018404
- TIGRFAMs:   TIGR00822

Pfam domain/function: PF03609 EII-Sor

EC number: NA

Molecular weight: Translated: 27636; Mature: 27636

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS51106 PTS_EIIC_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x18eb47d8)-; HASH(0x18cae900)-; HASH(0x17b1ccec)-; HASH(0x188f65dc)-; HASH(0x18ba4ab0)-; HASH(0x18cb9c34)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure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CCCCCCHHCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796