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The map label for this gene is hcr [H]

Identifier: 170683685

GI number: 170683685

Start: 906081

End: 907049

Strand: Reverse

Name: hcr [H]

Synonym: EcSMS35_0901

Alternate gene names: 170683685

Gene position: 907049-906081 (Counterclockwise)

Preceding gene: 170683671

Following gene: 170679672

Centisome position: 17.9

GC content: 55.73

Gene sequence:

>969_bases
ATGACGATGCCAACGAATCAATGCCCGTGGCGGATGCAGGTTCATCACATTACGCAAGAAACGCCGGATGTGTGGACGAT
TTCCCTGATTTGCCACGATTACTACCCATATCGCGCCGGGCAATATGCACTGGTCAGCGTGCGTAACTCAGCGGAAACGC
TGCGTGCTTACACCATTTCCTCCACGCCAGGCGTGAGTGAATACATCACCCTGACTGTGCGGCGGATTGATGACGGTGTC
GGCTCCCGGTGGCTGACGCGCGATGTAAAACGCGGTGATTATCTCTGGCTTTCGGACGCGATGGGGGAATTTACCTGCGA
CGATAAAGCAGAAGATAAATTCCTGTTGCTGGCGGCAGGCTGCGGCGTTACGCCGATTATGTCGATGCGTCGCTGGCTGG
CGAAGAACCGTCCACAGGCCGATGTGCAGGTGATCTACAACGTGCGTACGCCGCAGGATGTGATTTTCGCCGATGAGTGG
CGTAACTATCCGGTAACGCTGGTGGCGGAAAATAACGTTACCGAAGGCTTTATCGCTGGTCGTCTCACTCGCGAACTGCT
GGCAGGTGTCCCTGATTTAGCCTCACGTACCGTGATGACCTGTGGCCCTGCTCCATATATGGATTGGGTAGAGCAGGAAG
TGAAAGCGCTCGGCGTGACGCGTTTCTTTAAAGAGAAATTCTTCACCCCAGTAGCGGAAGCGGCGACCAGCGGTCTGAAA
TTCACCAAACTGCAACCGGCACGAGAATTTTACGCCCCGGTTGGCACCACGCTACTGGAGGCGCTGGAAAGCAATAACGT
TCCGGTTGTCGCCGCCTGCCGCGCAGGTGTTTGCGGCTGCTGTAAGACGAAAGTGGTTTCCGGTGAATATACGGTGAGCA
GCACAATGACGCTGACCGACGCCGAAATCGCTGAAGGTTACGTACTGGCCTGCTCCTGCCATCCGCAGGGGGATTTGGTT
CTCGCATAA

Upstream 100 bases:

>100_bases
CAGACCTGCTGGCAGTGCTGAACGAGAAATTCGGCCTGCGTTCTATCACCACTGTTGAAGAAGACATGAAGCAACTGTTG
AGCGCGTAAGGAGTTTGTCG

Downstream 100 bases:

>100_bases
TCGCCTGATGCCCGATGATATTCCTTTCATCGGGCTATTTAACCGTAAGTGCCTCCTTTTTCTCCCATCCCTTCCCCCTC
CGTCAGATGAACTAAACTTG

Product: HCP oxidoreductase, NADH-dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGV
GSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLV
LA

Sequences:

>Translated_322_residues
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGV
GSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLV
LA
>Mature_321_residues
TMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVG
SRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEWR
NYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLKF
TKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVL
A

Specific function: NADH oxidoreductase acting in concert with HCP [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787098, Length=322, Percent_Identity=99.3788819875776, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1787658, Length=338, Percent_Identity=28.4023668639053, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788104, Length=289, Percent_Identity=28.3737024221453, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 35740; Mature: 35609

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTIS
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEC
STPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAG
CCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
CGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEWRNYPVTLVAENNVTEGFIAG
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCCCHHHHH
RLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
HHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCE
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTD
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCEEEEEEEEEEC
AEIAEGYVLACSCHPQGDLVLA
HHHCCCEEEEEEECCCCCEEEC
>Mature Secondary Structure 
TMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYALVSVRNSAETLRAYTIS
CCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEC
STPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMGEFTCDDKAEDKFLLLAAG
CCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
CGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEWRNYPVTLVAENNVTEGFIAG
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCCCHHHHH
RLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALGVTRFFKEKFFTPVAEAATSGLK
HHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCE
FTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTD
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCEEEEEEEEEEC
AEIAEGYVLACSCHPQGDLVLA
HHHCCCEEEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10651802 [H]