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The map label for this gene is recJ [H]

Identifier: 170683389

GI number: 170683389

Start: 3115173

End: 3116906

Strand: Reverse

Name: recJ [H]

Synonym: EcSMS35_3025

Alternate gene names: 170683389

Gene position: 3116906-3115173 (Counterclockwise)

Preceding gene: 170682633

Following gene: 170682698

Centisome position: 61.5

GC content: 56.98

Gene sequence:

>1734_bases
GTGAAACAACAGATACAACTTCGTCGCCGTGAAGTCGATGAAACGGCAGACTTGCCCGCGGAATTGCCTCCCTTGCTGCG
CCGTTTATACGCCAGCCGGGGAGTACGCAGTGCGCATGAACTGGAACGCAGTGTTAAAGGTATGCTGCCCTGGCAGCAAC
TGAGCGGCGTCGAAAAGGCCGTTGAGATCCTTTACAACGCTTTTCGCGAAGGAACGCGGATTATTGTGGTCGGTGATTTC
GACGCCGACGGCGCGACCAGCACGGCTCTAAGCGTGCTGGCGATGCGCTCGCTTGGTTGCAGCAATATCGACTACCTGGT
ACCAAACCGTTTCGAAGACGGTTACGGCTTAAGCCCGGAAGTAGTCGATCAGGCCCATGCCCGTGGCGCGCAGTTAATTG
TCACGGTGGATAACGGTATTTCCTCCCATGCGGGGGTTGAACACGCTCGTTCGTTGGGCATTCCGGTTATTGTTACCGAT
CACCATTTGCCGGGCGACACATTACCCGCAGCGGAAGCGATCATTAACCCTAACTTGCGCGACTGTAATTTCCCGTCGAA
ATCACTGGCAGGCGTGGGTGTGGCGTTTTATCTGATGCTGGCTCTGCGCACCTTTTTGCGCGATCAGGGCTGGTTTGATG
AGCGCGGCATCGCAATTCCTAATCTGGCAGAACTGCTGGATCTGGTGGCGTTGGGGACAGTGGCAGACGTGGTGCCGCTG
GACGCTAATAACCGTATTCTGACCTGGCAGGGGATGAGCCGCATCCGCGCCGGGAAGTGCCGTCCGGGGATTAAAGCGCT
GCTGGAAGTGGCCAATCGCGATGCGCAAAAACTCGCCGCCAGCGATTTAGGTTTTGCGCTGGGGCCGCGTCTCAATGCCG
CCGGGCGACTGGATGATATGTCCGTTGGTGTGGCGCTGTTGCTGTGCGACAACATCGGCGAAGCGCGCGTGCTGGCAAAT
GAACTCGATGCGCTAAACCAGACGCGAAAAGAGATCGAACAGGGAATGCAGATTGAAGCCCTGACCCTGTGCGAGAAACT
GGAGCGCAGCCGCGACACGCTACCCGGTGGGCTGGCAATGTATCACCCCGAATGGCATCAGGGCGTGGTCGGCATTCTGG
CTTCGCGCATCAAAGAGCGTTTTCATCGTCCGGTTATCGCCTTTGCGCCAGCAGGTGATGGTACGCTGAAAGGTTCCGGT
CGCTCCATTCAGGGGCTGCATATGCGTGATGCGCTGGAGCGGTTAGACATGCTCTACCCTGGCATGATGCTCAAGTTTGG
CGGTCATGCGATGGCGGCTGGTTTGTCGCTGGAAGAGGATAAATTCGAACTCTTTCAACAACGGTTTGGCGAACTGGTTA
CTGAGTGGCTGGATCCTTCACTATTGCAAGGCGAAGTGGTGTCAGACGGCCCGTTAAGCCCGGCCGAAATGACCATGGAA
GTAGCGCAGCTGCTGCGCGATGCTGGCCCGTGGGGGCAGATGTTCCCGGAGCCGCTGTTTGACGGTCATTTCCGCCTTCT
GCAACAACGACTGGTAGGCGAACGCCATTTGAAGGTCATGGTTGAACCGGTCGGCGGCGGCCCGCTGCTGGACGGCATTG
CCTTTAACGTTGATACCGCCCTTTGGCCGGATAACGGCGTGCGCGAAGTGCAACTGGCTTATAAGCTTGATATCAACGAG
TTTCGCGGCAATCGCAGCCTGCAAATTATCATCGACAATATCTGGCCAATTTAG

Upstream 100 bases:

>100_bases
TGCTGAGCAATGGCACACTTGTTCCGGGTTACCAGCCGCCGAAAGACATGAAAGAATTCCTCGACGAACACCAAAAAATG
ACCAGCGGTAAATAATTCGC

Downstream 100 bases:

>100_bases
CGTCATCTTCTCTATAAAAAAGAGCGTGGATTGGGTACAATCCCGCTCTTATCACCGCATTTTGACTAGCTCAAGAAAAG
AAATCAGACCATGTTTGAAA

Product: ssDNA exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAHELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDMLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI

Sequences:

>Translated_577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAHELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDMLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI
>Mature_577_residues
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAHELERSVKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDF
DADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLAN
ELDALNQTRKEIEQGMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSG
RSIQGLHMRDALERLDMLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINE
FRGNRSLQIIIDNIWPI

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=577, Percent_Identity=99.3067590987868, Blast_Score=1159, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63373; Mature: 63373

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAHELERSVKGMLPWQQLSGVEKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
VVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTDHHLPGDTLPAAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCCC
DCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEC
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDM
CCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNIGEARVLANELDALNQTRKEIEQGMQIEALTLCEKLERSRDTLPGGLAM
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDMLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
CHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHCCEEECCCCE
LWPDNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI
ECCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAHELERSVKGMLPWQQLSGVEKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSNIDYLVPNRFEDGYGLSPE
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
VVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTDHHLPGDTLPAAEAIINPNLR
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCCC
DCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEC
DANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDM
CCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCCCCCCCCHH
SVGVALLLCDNIGEARVLANELDALNQTRKEIEQGMQIEALTLCEKLERSRDTLPGGLAM
HHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
YHPEWHQGVVGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDMLYP
ECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GMMLKFGGHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTME
CHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHH
VAQLLRDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHCCEEECCCCE
LWPDNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI
ECCCCCCEEEEEEEEECHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987126; 9278503; 2649886 [H]