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The map label for this gene is fadI [H]

Identifier: 170683369

GI number: 170683369

Start: 2547857

End: 2549167

Strand: Reverse

Name: fadI [H]

Synonym: EcSMS35_2501

Alternate gene names: 170683369

Gene position: 2549167-2547857 (Counterclockwise)

Preceding gene: 170683882

Following gene: 170682519

Centisome position: 50.3

GC content: 55.84

Gene sequence:

>1311_bases
ATGGGTCAGGTTTTACCGCTGGTTACCCGCCTGGGCGATCGTATCGCCATTGTTAGCGGTTTACGTACGCCTTTTGCCCG
CCAGGCGACGGCTTTTCATGGCATTCCCGCGGTTGATTTAGGGAAGATGGTGGTAGGCGAACTACTAGCACGCAGCGAAA
TTCCCGCCGAAGTGATTGAACAACTGGTCTTTGGTCAGGTCGTTCAGATGCCGGAAGCCCCCAACATTGCGCGTGAAATT
GTTCTTGGCACCGGAATGAATGTCCATACCGATGCTTACAGCGTCAGCCGCGCTTGCGCTACCAGTTTCCAGGCGATTGC
AAACGTCGCAGAAAGTCTGATGGCGGGAACTATTCGCGCGGGGATTGCCGGTGGGGCCGATTCCTCTTCCGTATTGCCCA
TTGGCGTTAGCAAAAAACTGGCGCGCGTGCTGGTTGATGTCAACAAAGCCCGCACAATGAGCCAGCGCCTGAAACTTTTT
TCTCGCTTACGTTTGCGTGACTTAATGCCCGTACCGCCTGCGGTAGCAGAATATTCTACCGGCTTGCGGATGGGGGACAC
CGCAGAGCAAATGGCGAAAACCTACGGCATCACCCGAGAACAGCAAGATGCATTAGCGCACCGTTCGCATCAGCGAGCCG
CTCAGGCGTGGTCAGACGGTAAACTCAAAGAAGAGGTGATGACTGCCTTTATCCCTCCTTATAAACAACCGCTTGCCGAA
GACAACAATATTCGCGGTAATTCCACGCTTGCTGATTACGCAAAGCTGCGTCCGGCGTTTGATCGCAAACACGGGACGGT
AACGGCAGCAAACAGTACACCGCTGACCGATGGCGCGGCAGCGGTGATCCTGATGACTGAATCGCGGGCGAAAGAATTAG
GGCTGGTACCGCTGGGGTATCTGCGCAGTTACGCATTTACTGCGATAGATGTCTGGCAGGACATGTTGCTCGGTCCGGCC
TGGTCAACGCCGCTGGCGCTGGAACGGGCCGGATTGACGATGAGCGATCTGACCTTGATCGATATGCACGAAGCCTTTGC
AGCTCAGACACTGGCGAATATTCAGTTGCTGGGTAGCGAACGTTTTGCTCGTGATGTACTGGGGCGTGCACATGCCACTG
GCGAAGTGGACGATAGCAAATTTAACGTGCTTGGCGGTTCGATTGCTTACGGGCATCCCTTCGCGGCGACCGGCGCGCGG
ATGATTACCCAGACATTGCATGAACTTCGCCGTCGCGGCGGTGGATTTGGTTTAGTGACCGCCTGTGCTGCGGGTGGGCT
TGGCGCGGCAATGGTTGTGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
GCTCCCACTTTCCCTTTTCTCCACTTGGCTAAATGTAAAAAAATGGTTAAGACTATGATCAGGTCAGACCACTTTATTTA
TTATTTTACAGGGGAGTGTT

Downstream 100 bases:

>100_bases
TGGAAATGGCATCAGCGTTTACTCTTAATGTTCGTCTGGACAACATTGCTATCATCACCATCGACGTACCGGGTGAGAAA
ATGAATACCCTGAAGGCGGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGQVLPLVTRLGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLAE
DNNIRGNSTLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE

Sequences:

>Translated_436_residues
MGQVLPLVTRLGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLAE
DNNIRGNSTLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE
>Mature_435_residues
GQVLPLVTRLGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIV
LGTGMNVHTDAYSVSRACATSFQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFS
RLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLAED
NNIRGNSTLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAW
STPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARM
ITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=41.8052256532066, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI4557237, Length=424, Percent_Identity=30.188679245283, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=32.093023255814, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=30.8962264150943, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI148539872, Length=427, Percent_Identity=29.5081967213115, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI194353979, Length=106, Percent_Identity=37.7358490566038, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=98.394495412844, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=35.4609929078014, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=32.2274881516588, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI48994986, Length=437, Percent_Identity=35.4691075514874, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=33.0985915492958, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=38.7706855791962, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=31.2206572769953, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=29.0476190476191, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=424, Percent_Identity=29.2452830188679, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=420, Percent_Identity=24.7619047619048, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325229, Length=441, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=385, Percent_Identity=29.6103896103896, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=41.5492957746479, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=29.6208530805687, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=27.4647887323944, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17648125, Length=430, Percent_Identity=29.5348837209302, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46516; Mature: 46385

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVLPLVTRLGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
CCCHHHHHHHCCCCEEEHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAIANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLAE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHCCCC
DNNIRGNSTLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCHHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHCHH
RFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVVEAE
HHHCCCCCEEEEEECC
>Mature Secondary Structure 
GQVLPLVTRLGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
CCHHHHHHHCCCCEEEHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAIANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLAE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHCCCC
DNNIRGNSTLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCHHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHCHH
RFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVVEAE
HHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA