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The map label for this gene is ypdF [H]

Identifier: 170683336

GI number: 170683336

Start: 2596354

End: 2597439

Strand: Reverse

Name: ypdF [H]

Synonym: EcSMS35_2537

Alternate gene names: 170683336

Gene position: 2597439-2596354 (Counterclockwise)

Preceding gene: 170681226

Following gene: 170679878

Centisome position: 51.25

GC content: 57.92

Gene sequence:

>1086_bases
ATGACATTACTCGCTTCGCTGCGCGACTGGCTTAAGGCGCAACAACTGGATGCGGTGCTTCTCTCCTCACGGCAGAACAA
ACAGCCGCATCTGGGGATCTCCACCGGATCAGGCTATGTGCTGATTAGCCGTGAAAGTGCGCACATTCTGGTGGACTCGC
GCTATTACGCGGATGTAGAAGCCCGCACGCAAGGCTACCAGCTGCATTTGCTTGACGCGACGCACACGCTTGCAACCATC
GCCAGGCAAATCATTGCCGATGAGCAGTTAAAAACGCTCGGTTTTGAAGGCCAGCAGGTGAGTTGGGAAACCGCGCACCG
CTGGCAGTCTGAACTCAATGCGAAACTGGTAAGCGCCACGCCGGATGTGCTGCGGCAAATCAAAACGCCAGAGGAGGTGG
AGAAAATCCGCCTCGCCTGTGGGATTGCCGATCGCGGTGCAGAGCATATTCGCCGCTTTATTCAGGCGGGAATGAGCGAG
CGCGAGATAGCCGCTGAACTGGAGTGGTTTATGCGCCAGCAGGGCGCAGAAAAAGCCTCTTTCGATACCATTGTCGCCAG
CGGCTGGCGTGGGGCGCTGCCGCACGGCAAAGCCAGCGACAAGATTGTTGCAGCGGGCGAGTTTGTCACTCTTGATTTTG
GTGCGCTGTATCAGGGCTACTGCTCTGATATGACGCGCACCTTGCTGGTGAATGGCGAAGGGGTGAGCGCCGAATCTCAC
CCGCTGTTTGACGTCTATCAGATTGTTTTGCAGGCACAGCTCGCGGCAATCTCCGCGATTCGCCCCGGCGTGCGCTGCCA
GCAGGTTGACGACGCCGCGCGCCGGGTGATTACCGAGGCTGGATTTGGCGACTATTTCGGTCATAACACCGGTCATGCTA
TCGGCATTGAAGTCCATGAAGGTCCGCGTTTTTCACCGCGGGACACCACGACGCTACAGCCAGGCATGTTACTGACCGTG
GAGCCGGGGATTTATTTGCCAGGGCAAGGGGGCGTGCGCATCGAAGATGTTGTGCTGGTCACCCCGCAAGGCGCAGAAGT
GCTCTACGCCATGCCGAAAACAGTGTTGCTCACGGGAGAGGCATAA

Upstream 100 bases:

>100_bases
TGGGAGCAGTCATTACTGCGCTGATGGTGGTGTTCCTGCGTCTGATGATGTTCCGCAAAGGCAAATTGTTAATCGATAGC
CTGTAAGAAGGACAATCCTG

Downstream 100 bases:

>100_bases
TGGATTTATCGCTATTAAAAGCGTTGAGCGAGGCAGATGCGATCGCCTCCTCGGAACAGGAAGTACGGCAGATCCTGCTG
GAAGAAGCGGATCGCCTGCA

Product: aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISRESAHILVDSRYYADVEARTQGYQLHLLDATHTLATI
ARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
PLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHNTGHAIGIEVHEGPRFSPRDTTTLQPGMLLTV
EPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA

Sequences:

>Translated_361_residues
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISRESAHILVDSRYYADVEARTQGYQLHLLDATHTLATI
ARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
PLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHNTGHAIGIEVHEGPRFSPRDTTTLQPGMLLTV
EPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA
>Mature_360_residues
TLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISRESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIA
RQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSER
EIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESHP
LFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHNTGHAIGIEVHEGPRFSPRDTTTLQPGMLLTVE
PGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA

Specific function: Hydrolyzes the N-terminal methionine when the next amino acid is alanine, proline or serine. The substrate preference for methionyl aminopeptidase activity is Pro > Ala > Ser. Also able to hydrolyze the Xaa-Pro peptide bond when the first amino acid is al

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family [H]

Homologues:

Organism=Homo sapiens, GI11559925, Length=254, Percent_Identity=30.3149606299213, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI149589008, Length=308, Percent_Identity=27.9220779220779, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI260593665, Length=308, Percent_Identity=27.9220779220779, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI264681563, Length=198, Percent_Identity=33.8383838383838, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI260593663, Length=243, Percent_Identity=29.6296296296296, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI93141226, Length=174, Percent_Identity=34.4827586206897, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI264681565, Length=195, Percent_Identity=32.3076923076923, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788728, Length=361, Percent_Identity=96.398891966759, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI1789275, Length=254, Percent_Identity=29.9212598425197, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786364, Length=207, Percent_Identity=25.6038647342995, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790282, Length=300, Percent_Identity=26, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508215, Length=312, Percent_Identity=28.2051282051282, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989583, Length=247, Percent_Identity=25.9109311740891, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17509539, Length=168, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321118, Length=285, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320922, Length=240, Percent_Identity=24.5833333333333, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19920384, Length=260, Percent_Identity=27.6923076923077, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357079, Length=294, Percent_Identity=27.891156462585, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137632, Length=172, Percent_Identity=34.3023255813954, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 39544; Mature: 39413

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISRESAHILVDSRYYADVE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEECCEEECCH
ARTQGYQLHLLDATHTLATIARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSAT
HCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCC
PDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSEREIAAELEWFMRQQGAEKAS
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
HHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHCEEEEEECCCCCCCCCC
PLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHNTGHAIGIEVHE
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECC
GPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGE
CCCCCCCCCCEECCCEEEEECCCEEECCCCCCEEEEEEEECCCCCEEEEECCCEEEEECC
A
C
>Mature Secondary Structure 
TLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISRESAHILVDSRYYADVE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEECCEEECCH
ARTQGYQLHLLDATHTLATIARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSAT
HCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCC
PDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSEREIAAELEWFMRQQGAEKAS
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
HHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHCEEEEEECCCCCCCCCC
PLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHNTGHAIGIEVHE
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECC
GPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGE
CCCCCCCCCCEECCCEEEEECCCEEECCCCCCEEEEEEEECCCCCEEEEECCCEEEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]