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The map label for this gene is nanT1 [H]

Identifier: 170683321

GI number: 170683321

Start: 3598009

End: 3599499

Strand: Reverse

Name: nanT1 [H]

Synonym: EcSMS35_3519

Alternate gene names: 170683321

Gene position: 3599499-3598009 (Counterclockwise)

Preceding gene: 170680563

Following gene: 170683961

Centisome position: 71.02

GC content: 55.6

Gene sequence:

>1491_bases
ATGAGTACTACAACCCAGAATATCCCGTGGTATCGCCATCTTAACCGCGCACAATGGCGGGCATTTTCCGCTGCCTGGTT
GGGATATCTGCTTGACGGTTTTGATTTCGTTTTAATCGCCCTGGTACTCACCGAAGTACAGGGTGAATTCGGGCTGACGA
CGGTGCAGGCGGCAAGTCTGATCTCCGCAGCCTTTATCTCTCGCTGGTTCGGCGGCCTGATGCTCGGCGCTATGGGTGAC
CGCTACGGGCGTCGTCTGGCAATGGTCACCAGCATCGTTCTCTTCTCGGCCGGGACGCTGGCCTGCGGCTTTGCGCCAGG
CTACATCACCATGTTTATCGCTCGTCTGGTCATCGGCATGGGGATGGCGGGTGAATACGGTTCCAGCGCCACCTATGTCA
TTGAAAGCTGGCCAAAGCATCTGCGTAACAAAGCCAGTGGTTTTCTGATTTCCGGCTTCTCAGTGGGGGCCGTCGTTGCC
GCTCAGGTCTATAGCCTGGTGGTTCCGGTCTGGGGCTGGCGTGCGCTGTTCTTTATCGGCATTTTGCCAATCATCTTTGC
TCTCTGGCTGCGTAAAAACATCCCGGAAGCGGAAGACTGGAAAGAGAAACACGGGGGTAAAGCACCGGTACGCACGATGG
TAGACATTCTTTACCGTGGTGAACATCGCATTGCCAATATCGTAATGACACTGGCGGCGGCTACTGCGCTGTGGTTCTGC
TTCGCCGGTAACCTGCAAAATGCCGCGATCGTCGCTGTTCTTGGGCTGTTATGCGCCGCAATCTTTATCAGCTTTATGGT
GCAGAGTACGGGCAAACGCTGGCCAACGGGCGTAATGCTGATGGTGGTCGTGTTGTTTGCTTTCCTCTACTCATGGCCGA
TTCAGGCGCTGCTGCCAACGTATCTGAAAACCGATCTGGCTTATGACCCGCATACTGTCGCCAATGTGCTGTTCTTTAGT
GGCTTTGGCGCGGCGGTGGGGTGCTGCGTCGGTGGCTTCCTCGGTGACTGGCTGGGAACCCGCAAAGCGTACGTTTGTAG
TCTGCTGGCCTCGCAGCTGCTGATTATTCCGGTATTTGCGATTGGCGGCGCAAACGTCTGGGTGCTCGGTCTGTTACTGT
TCTTCCAGCAAATGCTTGGACAAGGGATCGCCGGGATCTTACCGAAACTGATTGGCGGTTATTTCGATACCGACCAGCGT
GCAGCGGGCCTGGGCTTTACCTACAACGTTGGCGCATTGGGCGGCGCACTGGCCCCAATCATCGGCGCGTTGATCGCTCA
ACGTCTGGATCTGGGTACTGCGCTGGCATCGCTCTCGTTCAGTTTGACGTTTGTGGTGATCCTGCTGATTGGTCTGGATA
TGCCTTCTCGCGTTCAGCGTTGGCTGCGCCCGGAAGCATTGCGTACTCATGACGCTATCGACGGCAAACCATTTAGCGGT
GCCGTGCCATTTGGCAGCGCCAAAAACGATTTAGTCAAAACCAAAAGTTAA

Upstream 100 bases:

>100_bases
CCCTCGCTCGCCCCTACCGGGTGAGGGGAAATAAACGCATCTGTACCCTACAATTTTCATACCAAAGCGTGTGGGCATCG
CCCACCGCGGGAGACTCACA

Downstream 100 bases:

>100_bases
TCCTGTTGCCCGGTCTATGTACCGGGCCTTTCGCTAAGGGAAGATGTATGTCGTTACTTGCACAACTGGATCAAAAAATC
GCTGCTAACGGTGGCCTGAT

Product: putative sialic acid transporter

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: Sialic acid permease 1 [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGD
RYGRRLAMVTSIVLFSAGTLACGFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILYRGEHRIANIVMTLAAATALWFC
FAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPTGVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFS
GFGAAVGCCVGGFLGDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQR
AAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDGKPFSG
AVPFGSAKNDLVKTKS

Sequences:

>Translated_496_residues
MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGD
RYGRRLAMVTSIVLFSAGTLACGFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILYRGEHRIANIVMTLAAATALWFC
FAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPTGVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFS
GFGAAVGCCVGGFLGDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQR
AAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDGKPFSG
AVPFGSAKNDLVKTKS
>Mature_495_residues
STTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDR
YGRRLAMVTSIVLFSAGTLACGFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVAA
QVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILYRGEHRIANIVMTLAAATALWFCF
AGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPTGVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSG
FGAAVGCCVGGFLGDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQRA
AGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDGKPFSGA
VPFGSAKNDLVKTKS

Specific function: May be a sugar-cation symporter involved in sialic acid uptake [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Sialic acid transporter (TC 2.A.1.12.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082231, Length=496, Percent_Identity=99.3951612903226, Blast_Score=986, Evalue=0.0,
Organism=Escherichia coli, GI87082404, Length=452, Percent_Identity=34.7345132743363, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790550, Length=483, Percent_Identity=24.223602484472, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788292, Length=227, Percent_Identity=25.1101321585903, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081723, Length=407, Percent_Identity=23.3415233415233, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788942, Length=296, Percent_Identity=26.0135135135135, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32564663, Length=188, Percent_Identity=27.6595744680851, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320595, Length=207, Percent_Identity=25.6038647342995, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004742
- InterPro:   IPR005828 [H]

Pfam domain/function: PF07690 MFS_1; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 53569; Mature: 53438

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQGEFGLTTVQAASL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLACGFAPGYITMFIARLVIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHC
GMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVAAQVYSLVVPVWGWRALFFIG
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILYRGEHRIANIVMTLAAATALWFC
HHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPTGVMLMVVVLFAFLYSWPIQALLPT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
YLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFLGDWLGTRKAYVCSLLASQLLIIPVFA
HHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGANVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQRAAGLGFTYNVGALGGALAPI
HCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHCCCCCEEEHHHHHHHHHHH
IGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDGKPFSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHCCCCCCCCCC
AVPFGSAKNDLVKTKS
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQGEFGLTTVQAASL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH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CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]