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The map label for this gene is amiC2 [H]

Identifier: 170683287

GI number: 170683287

Start: 3227446

End: 3228705

Strand: Reverse

Name: amiC2 [H]

Synonym: EcSMS35_3140

Alternate gene names: 170683287

Gene position: 3228705-3227446 (Counterclockwise)

Preceding gene: 170682595

Following gene: 170681295

Centisome position: 63.7

GC content: 51.67

Gene sequence:

>1260_bases
ATGCTGGTATCTAACTATAATTATGTAATCAGCCGTCGTCGTTTACTGCAAGGTGCGGGCGCCATGTGGCTATTGAGCGT
AAGTCAGGTCAGCCTGGCTGCGGTCAGCCAGGTCGTGGCGGTACGCGTCTGGCCTGCGTCCAGCTACACCCGCGTGACGG
TAGAATCAAATCGTCAGCTGCAATATAAGCAGTTCGCGTTAAGTAACCCTGAACGTGTGGTGGTGGATATCGAAGATGTA
AACCTGAACTCGGTACTCAAGGGGATGGCGGCACAAATTCGTGCAGACGACCCGTTCATCAAGTCGGCGCGCGTCGGGCA
ATTTGACCCGCAAACCGTACGCATGGTTTTTGAATTAAAGCAAAACGTAAAACCGCAGCTGTTTGCCCTTGCGCCGGTCG
CCGGGTTTAAAGAGCGTCTGGTGATGGACCTCTATCCGGCCAATGCACAGGATATGCAGGACCCGCTGCTGGCGCTGCTG
GAGGATTACAACAAAGGCGACCTCGAAAAGCAGGTGCCGCCAGCACAAAGTGGTCCACAACCGGGTAAAGCAGGGCGTGA
TCGTCCGATTGTCATTATGCTTGACCCCGGCCACGGTGGCGAAGACCCCGGTGCGGTGGGGAAATACAAAACACGCGAAA
AAGACGTGGTATTGCAAATAGCTCGCCGTCTGCGCTCTCTGATCGAAAAAGAGGGTAATATGAAGGTGTACATGACGCGC
AATGAAGACATCTTCATTCCGTTGCAAGTGCGTGTAGCAAAAGCACAGAAACAACGTGCTGACCTTTTTGTTTCTATCCA
TGCTGATGCCTTTACCAGTCGCCAGCCGAGCGGTTCTTCCGTGTTTGCGCTTTCGACCAAAGGCGCGACAAGTACTGCGG
CAAAATATCTGGCACAAACCCAGAACGCCTCGGACTTGATTGGCGGCGTGAGCAAAAGCGGTGACCGCTATGTCGACCAC
ACCATGTTCGATATGGTGCAGTCTCTGACCATTGCCGACAGCCTGAAGTTGGGTAAAGCGGTGCTGAATAAGCTCGGTAA
AATCAACAAGCTGCATAAAAATCAGGTTGAACAGGCCGGGTTTGCCGTGCTGAAAGCACCTGATATTCCCTCCATTCTGG
TCGAAACGGCGTTTATCAGTAACGTTGAGGAAGAGCGCAAACTGAAAACGGCAAGATTCCAACAACAAGTGGCAGAATCT
ATCCTAGAGGGAATTAAAGAGTATTTTTCGGATATAGAAGCGTTAGCGAGAAAAGCATAA

Upstream 100 bases:

>100_bases
ATTGCAAAGATAAGAATACGTGTGATTATTTCTTTAAACACCTGTAAAAATTTATTAGAGCATAGTTATATTATTGAACA
TTGTATTGCTTAGGTAAGGT

Downstream 100 bases:

>100_bases
TTTAAAAATGTTTAATATTCTATGTTCTCGGTCATATTTTATTAATTTGGATGAATATATGCTGTTAGTGCTGTATTCTC
CGTTTGGATACACCTTTACC

Product: N-acetylmuramoyl-L-alanine amidase AmiC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLVSNYNYVISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRVTVESNRQLQYKQFALSNPERVVVDIEDV
NLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALL
EDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTR
NEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDH
TMFDMVQSLTIADSLKLGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTARFQQQVAES
ILEGIKEYFSDIEALARKA

Sequences:

>Translated_419_residues
MLVSNYNYVISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRVTVESNRQLQYKQFALSNPERVVVDIEDV
NLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALL
EDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTR
NEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDH
TMFDMVQSLTIADSLKLGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTARFQQQVAES
ILEGIKEYFSDIEALARKA
>Mature_419_residues
MLVSNYNYVISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRVTVESNRQLQYKQFALSNPERVVVDIEDV
NLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALL
EDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTR
NEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDH
TMFDMVQSLTIADSLKLGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTARFQQQVAES
ILEGIKEYFSDIEALARKA

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=417, Percent_Identity=95.6834532374101, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1788776, Length=236, Percent_Identity=48.3050847457627, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=41.3333333333333, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46303; Mature: 46303

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVSNYNYVISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRVTVESNRQL
CEECCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCE
QYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELK
EHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
QNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQ
HCCCCEEEEECCCHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PGKAGRDRPIVIMLDPGHGGEDPGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTR
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQT
CCCEEEEEEEHHHHHHHHCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
QNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKLGKAVLNKLGKINKLHKNQVEQAG
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FAVLKAPDIPSILVETAFISNVEEERKLKTARFQQQVAESILEGIKEYFSDIEALARKA
EEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVSNYNYVISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRVTVESNRQL
CEECCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCE
QYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELK
EHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
QNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQ
HCCCCEEEEECCCHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PGKAGRDRPIVIMLDPGHGGEDPGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTR
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQT
CCCEEEEEEEHHHHHHHHCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
QNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKLGKAVLNKLGKINKLHKNQVEQAG
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FAVLKAPDIPSILVETAFISNVEEERKLKTARFQQQVAESILEGIKEYFSDIEALARKA
EEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]