Click here to switch to the map view.

The map label for this gene is pbpC [H]

Identifier: 170683227

GI number: 170683227

Start: 2740971

End: 2743283

Strand: Reverse

Name: pbpC [H]

Synonym: EcSMS35_2671

Alternate gene names: 170683227

Gene position: 2743283-2740971 (Counterclockwise)

Preceding gene: 170682175

Following gene: 170680359

Centisome position: 54.13

GC content: 56.42

Gene sequence:

>2313_bases
ATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGCTGGCAGCTGCGCCATTTCTCCTTTTTCTTGCAGCGTGGGG
AGCAGATAAACTCTGGCCTCTACCGCTGCATGAAGTCAATCCCGCACGAGTGGTTGTGGCGCAGGATGGGACGCCGCTCT
GGCGCTTTGCCGATGCGGAAGGCATCTGGCGTTATCCGGTAACAATCGAAGATGTTTCTCCACGTTACCTTGAAGCGCTG
ATCAATTATGAAGATCGCTGGTTCTGGAAGCATCCGGGGGTGAATCCATTCTCGGTGGCGCGCGCAGCATGGCAAGATCT
CACTTCGGGAAGGGTTATTTCCGGTGGCAGCACGCTCACTATGCAGGTTGCTCGTCTGCTTGATCCTCACCCCAAAACAT
TTGGCGGCAAAATTCGCCAGCTCTGGCGCGCGTTGCAACTGGAGTGGCATCTGTCTAAGCGTGAAATTCTGACCTTGTAT
CTTAACCGCGCTCCGTTTGGCGGTACGTTGCAGGGGATCGGTGCGGCAAGTTGGGCTTATCTCGGAAAATCGCCTGCGAA
TTTAAGCTATTCCGAGGCGGCAATGCTGGCGGTGTTGCCGCAAGCGCCCAGCCGACTGCGCCCGGATCGCTGGCCGGAAC
GTGCCGAAGCCGCGCGTAATAAAGTGCTCGAAAGGATGGCCGTGCAAGGTGTGTGGTCCCGTGAGCAGGTAAAAGAGTCA
AGGGAAGAACCCATCTGGCTGGCACCCCGACAAATGCCGCAACTGGCACCGCTGTTTTCGCGCATGATGCTCGGTAAAAG
CAAAAGCGACAAAATCGTTACGACGCTGGATGCCGGTCTTCAACGACGTCTGGAAGAACTGGCGCAAAACTGGAAAGGAC
GATTACCACCGCGCAGCTCACTGGCGATGATCGTGGTTGATCATACCGATATGCGTGTTCGCGGCTGGGTGGGTTCGGTT
GATCTCAACGATGATTCACGCTTTGGTCATGTTGATATGGTCAATGCGATCCGATCGCCAGGATCGGTGCTCAAACCGTT
TGTTTATGGTCTGGCGCTGGATGAAGGCTTGATCCACCCGGCATCGCTGTTGCAAGACGTCCCCCGACGCACCGGTGATT
ACCGACCTGGCAACTTTGACAGCGGTTTTCATGGCCCGATCAGCATGAGTGAGGCGCTGGTGCGATCGCTGAACTTACCT
GCTGTGCAGGTGCTGGAAGCCTATGGACCGAAACGGTTTGCGGCAAAGTTACGCAATGTTGGATTGCCGTTATATTTGCC
CAACGGTGCTGCACCGAATCTTTCGCTCATTCTCGGCGGCGCTGGCGCAAAACTGGAAGATATGGCGGCAGCGTATACCG
CGTTTGCTCGCCACGGCAAGGCAGGCAAATTGCGCTTACAGCCTGACGATCCGCTGCTTGAACGGCCTTTAATGTCGTCG
GGGGCTGCGTGGATCATTCGCCGGATTATGGCTGATGAAGCGCAACCCTTGCCGGATGGTGCCTTGCCGCGCGTCGCCCC
ACTGGCATGGAAAACGGGCACCAGCTATGGCTATCGTGATGCCTGGGCGATTGGGGTTAACGCTCGCTATGTCATTGGGA
TCTGGACTGGCAGACCGGACGGCACGCCCGTTGTTGGTCAGTTTGGCTTTGCCAGCGCCGTACCATTGTTGAATCAGGTC
AATAATATCTTACTGTCGCGCAGTGCGAATCTGCCGGAAGACCCACGTCCTGACTCTGTAAGTCGTGGCGTTATCTGCTG
GCCGGGCGGACAGTCTTTGCCGGAAGGTGACAGTAACTGTCGCCGCCGCCTGGCAACCTGGCTGCTGGACGGGAGTCAGC
CGCCAACTCTATTACTGCCGGAGCAGGAAGGCATTAATGGCATTCGTTTCCCCATCTGGCTGGATGAAAACGGCAAACGT
GTTGCCGCTGATTGCCCTCAGGCGCGACAAGAAATGATAAATGTCTGGCCGCTACCGCTGGAACCCTGGCTGCCCGCATC
AGAACGTCGCGCTGTGCGCTTGCCACCGGCCTCGACAATTTGCCCGCCATACGGTCACGATGCACAACTCCCGCTGCAGC
TAACAGGTGTGCGCGATGGCGCGATTATTAAACGTTTACCGGGCGCAGCGGAAGCGACTTTGCCGTTACAATCAAGTGGA
GGGGCAGGTGAACGCTGGTGGTTTCTGAACGGCGAACCGTTAACTGAACGTGGGCGCAACGTGACCCTGCATTTGACGGA
TAAAGGCGATTATCAATTGCTGGTGATGGATGAGGTGGGGCAAATAGCGACAGTGAAATTTGTCATGCAATAG

Upstream 100 bases:

>100_bases
GGGAACGTATCAGGTACCGCAACCGATGGTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATT
TGCTAATTGTCAGACCGTAA

Downstream 100 bases:

>100_bases
TCTGTTTTGTCCCTGATTGTTGCTTAAACTGGGTTTATTTTAAAAAAATGTTACCTTCATCAATAGTCAACGGCTCTGTT
GCTCATTATAATCCGCGCCA

Product: penicillin-binding protein 1C

Products: NA

Alternate protein names: PBP-1c; PBP1c; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Transpeptidase-like module [H]

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARVVVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEAL
INYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNKVLERMAVQGVWSREQVKES
REEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSV
DLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVRSLNLP
AVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSS
GAAWIIRRIMADEAQPLPDGALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQV
NNILLSRSANLPEDPRPDSVSRGVICWPGGQSLPEGDSNCRRRLATWLLDGSQPPTLLLPEQEGINGIRFPIWLDENGKR
VAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSG
GAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDEVGQIATVKFVMQ

Sequences:

>Translated_770_residues
MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARVVVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEAL
INYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNKVLERMAVQGVWSREQVKES
REEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSV
DLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVRSLNLP
AVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSS
GAAWIIRRIMADEAQPLPDGALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQV
NNILLSRSANLPEDPRPDSVSRGVICWPGGQSLPEGDSNCRRRLATWLLDGSQPPTLLLPEQEGINGIRFPIWLDENGKR
VAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSG
GAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDEVGQIATVKFVMQ
>Mature_769_residues
PRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARVVVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALI
NYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLYL
NRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNKVLERMAVQGVWSREQVKESR
EEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVD
LNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVRSLNLPA
VQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSSG
AAWIIRRIMADEAQPLPDGALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVN
NILLSRSANLPEDPRPDSVSRGVICWPGGQSLPEGDSNCRRRLATWLLDGSQPPTLLLPEQEGINGIRFPIWLDENGKRV
AADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGG
AGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDEVGQIATVKFVMQ

Specific function: Cell wall formation. The enzyme has a penicillin- insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a transpeptidase C-terminal domain which may not be functional [H]

COG id: COG4953

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein PbpC

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1788867, Length=770, Percent_Identity=98.8311688311688, Blast_Score=1534, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=261, Percent_Identity=34.4827586206897, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI1786343, Length=515, Percent_Identity=27.1844660194175, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011815
- InterPro:   IPR009647
- InterPro:   IPR001460 [H]

Pfam domain/function: PF06832 BiPBP_C; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 85091; Mature: 84960

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARVVVAQDGTPLWRFADAE
CCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCC
GIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLT
CEEECCEEEECCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCCHHH
MQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLYLNRAPFGGTLQGIGAASWAY
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHH
LGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNKVLERMAVQGVWSREQVKES
HCCCCCCCCHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
REEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSS
HCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
LAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHP
EEEEEEECCCEEEEEEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCH
ASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVRSLNLPAVQVLEAYGPKRFAAKLRNV
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHC
GLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSS
CCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCHHCC
GAAWIIRRIMADEAQPLPDGALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEECCCC
GTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPDSVSRGVICWPGGQSLPEGDSNC
CCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHH
RRRLATWLLDGSQPPTLLLPEQEGINGIRFPIWLDENGKRVAADCPQARQEMINVWPLPL
HHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHEECCCCC
EPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSG
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCCCCEEEEECCCC
GAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDEVGQIATVKFVMQ
CCCCEEEEECCCCHHHCCCEEEEEEECCCCEEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure 
PRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARVVVAQDGTPLWRFADAE
CCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCC
GIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLT
CEEECCEEEECCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCCHHH
MQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLYLNRAPFGGTLQGIGAASWAY
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHH
LGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNKVLERMAVQGVWSREQVKES
HCCCCCCCCHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
REEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSS
HCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
LAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHP
EEEEEEECCCEEEEEEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCH
ASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVRSLNLPAVQVLEAYGPKRFAAKLRNV
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHC
GLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSS
CCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCHHCC
GAAWIIRRIMADEAQPLPDGALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEECCCC
GTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPDSVSRGVICWPGGQSLPEGDSNC
CCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHH
RRRLATWLLDGSQPPTLLLPEQEGINGIRFPIWLDENGKRVAADCPQARQEMINVWPLPL
HHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHEECCCCC
EPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSG
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCCCCEEEEECCCC
GAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDEVGQIATVKFVMQ
CCCCEEEEECCCCHHHCCCEEEEEEECCCCEEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10542235; 9205837; 9278503; 9841666 [H]