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The map label for this gene is ascG [H]

Identifier: 170683113

GI number: 170683113

Start: 2913420

End: 2914472

Strand: Reverse

Name: ascG [H]

Synonym: EcSMS35_2838

Alternate gene names: 170683113

Gene position: 2914472-2913420 (Counterclockwise)

Preceding gene: 170679781

Following gene: 170682276

Centisome position: 57.5

GC content: 52.61

Gene sequence:

>1053_bases
GTGCAGTACACTGCGTTGATTGCGTGTAAAGGGATGAAAATGATGACGACGATGCTGGAAGTGGCGAAGCGCGCCGGGGT
TTCAAAAGCGACCGTTTCCCGCGTGCTTTCAGGTAATGGCTACGTCAGCCAGGAGACTAAAGATCGCGTGTTTCAGGCGG
TAGAAGAGAGCGGTTACCGCCCAAATTTGCTGGCCCGCAATCTGTCGGCGAAAAGTACCCAGACGCTGGGGCTGGTAGTG
ACTAACACGCTTTACCACGGCATTTATTTTAGTGAACTACTGTTTCATGCCGCGCGAATGGCGGAAGAGAAAGGGCGGCA
GTTGCTATTGGCAGATGGTAAACACAGCGCCGAAGAAGAGCGCCAGGCGATTCAGTATCTGCTGGATCTGCGCTGCGACG
CGATCATGATTTATCCGCGCTTTTTAAGCGTGGATGAAATTGATGACATCATTGACGCGCACAGTCAGCCGATAATGGTG
CTTAATCGCCGCCTGCGCAAAAATAGCAGCCATAGCGTCTGGTGCGATCATAAACAGACCAGCTTTAACGCCGTGGCAGA
GTTGATAAACGCCGGGCATCAGGAGATTGCTTTCCTTACCGGCTCGATGGATTCCCCCACCAGCATTGAACGTCTTGCCG
GGTATAAAGACGCGCTGTCGCAGCATGGTATTGCGCTCAATGAAAAACTTATCGCTAACGGTAAATGGACGCCTGCCAGC
GGGGCCGAAGGGGTAGAAACGTTGCTCGAACGTGGGGCTAAATTTAGCGTGTTAGTTGCCAGTAACGACGATATGGCGAT
AGGTGCGATGAAAGCGTTGCACGAGCGCGGCGTAGCGGTGCCAGAGCAGGTGTCAGTTATCGGATTCGATGATATCGCTA
TTGCCCCCTACACCGTTCCGGCGCTCTCCAGCGTAAAAATTCCGGTAACTGAGATGATTCAGGAAATTATTGGACGGCTG
ATTTTTATGCTCGATGGTGGGGATTTCTCACCGCCGAAAACCTTCAGCGGAAAACTGATCCGCCGCGGCTCCCTCATTGC
TCTTTCGCAATAA

Upstream 100 bases:

>100_bases
CACTAAATAAGCAAGAGAGTTAATTGTTCATTTTTTATATTTGTGAAACCGGTCACCTGAATATTTTTCCCGCCGATTGC
GCAAGGACAATCGGCAAACG

Downstream 100 bases:

>100_bases
ACATTGTCATCACATGTCATCGTCACTAGTGTCGATGACATCGTTTTTCCTGCATTACAGAATTTAATTTCTTTAAATAA
CAGTAAATTAAAAACTGGCA

Product: transcriptional regulator AscG

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVV
TNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMV
LNRRLRKNSSHSVWCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPAS
GAEGVETLLERGAKFSVLVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRL
IFMLDGGDFSPPKTFSGKLIRRGSLIALSQ

Sequences:

>Translated_350_residues
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVV
TNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMV
LNRRLRKNSSHSVWCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPAS
GAEGVETLLERGAKFSVLVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRL
IFMLDGGDFSPPKTFSGKLIRRGSLIALSQ
>Mature_350_residues
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVV
TNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMV
LNRRLRKNSSHSVWCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPAS
GAEGVETLLERGAKFSVLVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRL
IFMLDGGDFSPPKTFSGKLIRRGSLIALSQ

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=98.2195845697329, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=35.3293413173653, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=33.033033033033, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=30.2052785923754, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787948, Length=301, Percent_Identity=29.9003322259136, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=28.4829721362229, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=27.3615635179153, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=27.9761904761905, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1790715, Length=295, Percent_Identity=25.4237288135593, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787906, Length=317, Percent_Identity=28.7066246056782, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=25, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789456, Length=303, Percent_Identity=26.4026402640264, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786268, Length=304, Percent_Identity=24.3421052631579, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38329; Mature: 38329

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYR
CCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEE
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
RQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCDHKQT
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCC
SFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPAS
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCHHHHHHHCCCEECCEEEECCCCCCCC
GAEGVETLLERGAKFSVLVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVP
CCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCC
ALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIALSQ
CCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEECCCEEEECC
>Mature Secondary Structure
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYR
CCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEE
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
RQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCDHKQT
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCC
SFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIANGKWTPAS
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCHHHHHHHCCCEECCEEEECCCCCCCC
GAEGVETLLERGAKFSVLVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVP
CCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCC
ALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIALSQ
CCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]