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The map label for this gene is pdxK [H]

Identifier: 170682925

GI number: 170682925

Start: 2628814

End: 2629665

Strand: Reverse

Name: pdxK [H]

Synonym: EcSMS35_2574

Alternate gene names: 170682925

Gene position: 2629665-2628814 (Counterclockwise)

Preceding gene: 170684234

Following gene: 170679738

Centisome position: 51.88

GC content: 51.64

Gene sequence:

>852_bases
ATGAGTAGTTTGTTGTTGTTTAACGATAAGAGCAGGGCACTGCAGGCGGATATCGTTGCCGTGCAGTCGCAGGTAGTGTA
CGGCAGCGTAGGCAACAGCATTGCGGTGCCTGCTATCAAACAGAACGGCCTGAATGTCTTTGCCGTGCCGACGGTATTGC
TGAGCAATACGCCGCATTATGACACTTTCTACGGTGGTGCGATTCCGGATGAATGGTTTAGCGGTTATTTGCGTGCGCTT
CAGGAGCGTGATGCGCTGCGCCAACTTCGTGCTGTAACCACGGGCTATATGGGAACGGCATCGCAAATCAAAATCCTTGC
CGAGTGGCTGACTGCGCTACGCAAAGACCATTCTGACTTATTGATTATGGTCGATCCGGTGATCGGCGATATTGATAGCG
GAATTTATGTCAAACCTGACCTTCCCGAAGCGTATCGACAATATTTACTGCCGCTGGCGCAGGGAATTACCCCCAATATC
TTTGAGCTGGAAATCCTGACCGGTAAAAATTGCCGCGATCTCGACAGTGCCATTGCTGCCGCAAAAAGTCTGCTTTCAGA
CACATTAAAATGGGTGGTGATTACCAGCGCCTCCGGTAATGAAGAAAATCAGGAGATGCAGGTGGTGGTGGTCAGTGCCG
ACAGCGTGAATGTCATTTCCCATTCACGGGTTAAAACTGACCTGAAAGGGACTGGCGACCTGTTTTGTGCTCAGCTTATC
AGTGGTTTGCTGAAAGGGGAGGCGTTAACCGATGCGGTGCACCGCGCGGGGTTGCGCGTACTGGAAGTGATGCGCTATAC
CCAGCAGCATGAGAGCGATGAATTGATTTTGCCGCCGCTGGCGGAAGCATAA

Upstream 100 bases:

>100_bases
TTACCTTATCCTTGTTTTGTAATAGCCGATTTGACTGCCAGAATCAGGTAAAGTTTCGGTTGGAATACGATGAGGATCAT
CGGCAAGGAGAAAAAATTAA

Downstream 100 bases:

>100_bases
AAAAATGGCGCCGATGGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGG
GCCTCTCCTTTACCACTCTC

Product: pyridoxal kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRAL
QERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHSDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
SGLLKGEALTDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA

Sequences:

>Translated_283_residues
MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRAL
QERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHSDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
SGLLKGEALTDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA
>Mature_282_residues
SSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQ
ERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHSDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIF
ELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLIS
GLLKGEALTDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG2240

COG function: function code H; Pyridoxal/pyridoxine/pyridoxamine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyridoxine kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505701, Length=241, Percent_Identity=27.3858921161826, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1788758, Length=283, Percent_Identity=98.5865724381625, Blast_Score=572, Evalue=1e-165,
Organism=Escherichia coli, GI1787924, Length=266, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17507759, Length=268, Percent_Identity=29.1044776119403, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17507757, Length=279, Percent_Identity=28.3154121863799, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320806, Length=236, Percent_Identity=26.6949152542373, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324354, Length=245, Percent_Identity=27.3469387755102, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45553007, Length=188, Percent_Identity=33.5106382978723, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004625 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 30839; Mature: 30707

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHY
CCCEEEECCCCHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCEEEEEHHHHHCCCCCC
DTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHSDL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCE
LIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIFELEILTGKNCRDLDSAIAA
EEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
HHHHHHHCEEEEEEECCCCCCCCCEEEEEEEECCCEEEHHHHHHHCCCCCCHHHHHHHHH
SGLLKGEALTDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
SSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHY
CCEEEECCCCHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCEEEEEHHHHHCCCCCC
DTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHSDL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCE
LIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIFELEILTGKNCRDLDSAIAA
EEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
HHHHHHHCEEEEEEECCCCCCCCCEEEEEEEECCCEEEHHHHHHHCCCCCCHHHHHHHHH
SGLLKGEALTDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA