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The map label for this gene is yphF [H]

Identifier: 170682923

GI number: 170682923

Start: 2773718

End: 2774701

Strand: Reverse

Name: yphF [H]

Synonym: EcSMS35_2701

Alternate gene names: 170682923

Gene position: 2774701-2773718 (Counterclockwise)

Preceding gene: 170682243

Following gene: 170683588

Centisome position: 54.75

GC content: 46.14

Gene sequence:

>984_bases
ATGCCTAAAAAAATGAGAACAACCCGTAATCTATTATTAATGGCTACGCTATTAGGTAGCGCACTATTTGCCAGGGCTGC
GGATAAAGAAATGACCATTGGCGCAATATACCTTGATACCCAGGGATATTACGCTGGCGTGCGTCAGGGTGTTCAGGATG
CGGCAAAAGATTCTTCAGTACAGGTACAGTTAATTGAAACTAACGCCCAGGGTGATATTTCGAAAGAAAGTACCTTTGTT
GATACCCTCGTGGCGCGTAATGTCGATGCCATTATTTTATCGGCAGTGTCTGAAAATGGCAGTAGCCGTACCGTTCGTCG
CGCCAGTGAAGCGGGTATTCCGGTGATTTGCTACAACACCTGTATTAATCAAAAAGGCGTCGATAAATATGTCTCGGCGT
ATCTGGTCGGCGATCCGCTGGAATTTGGTAAAAAACTGGGTAATGCTGCCGCCGATTATTTTATTGCCAACAAAATTGAC
CAGCCGAAAATTGCCGTCATCAATTGCGAAGCCTTTGAAGTTTGTGTGCAGCGACGTAAAGGGTTTGAAGAAGTATTAAA
AGCCCGCGTTCCCGGCGCGCAAATTGTCGCTAATCAGGAAGGGACTGTTTTAGATAAAGCGATTTCCGTTGGTGAAAAAC
TAATTATCTCCAGGCCGGATCTCAACGCCATTATGGGTGAATCGGGCGGAGCGACACTCGGCGCGGTAAAAGCGGTACGT
AATCAAAATCAGGCCGGAAAAATTGCTGTTTTCGGTTCGGATATGACCACTGAAATTGCTCAGGAGCTGGAAAACAATCA
GGTGCTGAAAGCGGTAGTGGATATTTCCGGCAAGAAAATGGGCAATGCTGTTTTCGCGCAAACATTGAAGGTTATCAATA
AACAAGCCGACGGTGAAAAAGTGATTCAGGTGCCTATCGATCTCTATACCAAAACGGAAGACGGTAAACAGTGGCTGGCA
ACGCACGTTGATGGTCTGCCCTAA

Upstream 100 bases:

>100_bases
CTAAACCCTATTAACTGTAATATTTGAGCGGCACGCGCTGCCGCTCACGCTCAAATAATTAAAATAAGCCGTTGTAAATA
AAAACCCTACAGGAGGCACT

Downstream 100 bases:

>100_bases
TCGCTGATTCGCAGAGGTCCCTATGTTCACGGCAACAGAGGCAGTCCCGGTAGCAAAAGTGGTGGCAGGAAATAAGCGTT
ATCCCGGCGTCGTTGCGTTG

Product: putative sugar ABC transporter periplasmic sugar-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFV
DTLVARNVDAIILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIISRPDLNAIMGESGGATLGAVKAVR
NQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLA
THVDGLP

Sequences:

>Translated_327_residues
MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFV
DTLVARNVDAIILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIISRPDLNAIMGESGGATLGAVKAVR
NQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLA
THVDGLP
>Mature_326_residues
PKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVD
TLVARNVDAIILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQ
PKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIISRPDLNAIMGESGGATLGAVKAVRN
QNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLAT
HVDGLP

Specific function: Probably part of the binding-protein-dependent transport system yphDEF [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788898, Length=327, Percent_Identity=98.7767584097859, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1788473, Length=268, Percent_Identity=25.7462686567164, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35111; Mature: 34979

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCE
QVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVRRASEAGIPVICYNT
EEEEEEECCCCCCCCHHHHHHHHHHCCHHHEEHHHHHCCCCCHHHHHHHHCCCCEEEEEC
CINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFEVCVQRRK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHC
GFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIISRPDLNAIMGESGGATLGAVKAVR
CHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHCCCCCCHHHHHHHHH
NQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEK
CCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCE
VIQVPIDLYTKTEDGKQWLATHVDGLP
EEEEEEEEEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
PKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCE
QVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVRRASEAGIPVICYNT
EEEEEEECCCCCCCCHHHHHHHHHHCCHHHEEHHHHHCCCCCHHHHHHHHCCCCEEEEEC
CINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFEVCVQRRK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHC
GFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIISRPDLNAIMGESGGATLGAVKAVR
CHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHCCCCCCHHHHHHHHH
NQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEK
CCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCE
VIQVPIDLYTKTEDGKQWLATHVDGLP
EEEEEEEEEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9298646 [H]