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The map label for this gene is tadA [H]

Identifier: 170682913

GI number: 170682913

Start: 2792575

End: 2793078

Strand: Reverse

Name: tadA [H]

Synonym: EcSMS35_2711

Alternate gene names: 170682913

Gene position: 2793078-2792575 (Counterclockwise)

Preceding gene: 170679764

Following gene: 170682234

Centisome position: 55.11

GC content: 54.37

Gene sequence:

>504_bases
TTGTCTGAAGTCGAATTTAGCCACGAATACTGGATGCGTCACGCGCTGACGCTGGCAAAGCGTGCCTGGGATGAGCGGGA
AGTGCCGGTCGGCGCGGTATTAGTGCATAACAATCGTGTTATTGGCGAAGGCTGGAACCGTCCGATTGGTCGACATGATC
CCACCGCACATGCAGAAATCATGGCCCTGCGGCAGGGCGGTCTGGTGATGCAAAATTATCGACTGATCGACGCCACGTTG
TATGTCACGCTTGAACCCTGTGTAATGTGTGCCGGAGCGATGATCCACAGTCGCATTGGTCGCGTGGTCTTTGGTGCGCG
TGACGCGAAAACTGGCGCTGCGGGATCTTTAATGGATGTGCTGCATCATCCGGGTATGAATCACCGAGTGGAAATTACCG
AAGGAATACTGGCGGATGAGTGCGCGGCGTTGCTCAGTGACTTCTTTCGCATGCGCCGCCAGGAAATTAAAGCGCAGAAA
AAAGCGCAATCCTCGACGGATTAA

Upstream 100 bases:

>100_bases
TTGGCGAGTAACCCCGCGCGGTGAACTCCAGCAACTGGAATAGAGTAAAGCATAGCGTCCGTGTATAATGCGCCGCGCTT
TTATAACCGGAGTTTTCTTT

Downstream 100 bases:

>100_bases
TTTTGTTTCTCTTCACTCCCTTTCCTGGAAAACAGCAGAGAGGGAGAGTGGGTCAAATAGTTTGATGACGACTGGGAAAG
AAACGAGAGAAAAGGAAATT

Product: tRNA-specific adenosine deaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATL
YVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
KAQSSTD

Sequences:

>Translated_167_residues
MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATL
YVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
KAQSSTD
>Mature_166_residues
SEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLY
VTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKK
AQSSTD

Specific function: Deaminates adenosine-34 to inosine in tRNA-Arg2 [H]

COG id: COG0590

COG function: function code FJ; Cytosine/adenosine deaminases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytidine and deoxycytidylate deaminase family [H]

Homologues:

Organism=Homo sapiens, GI124107608, Length=151, Percent_Identity=33.7748344370861, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI145693172, Length=167, Percent_Identity=100, Blast_Score=348, Evalue=1e-97,
Organism=Escherichia coli, GI1786616, Length=168, Percent_Identity=32.7380952380952, Blast_Score=64, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17541138, Length=155, Percent_Identity=38.7096774193548, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554608, Length=97, Percent_Identity=38.1443298969072, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322425, Length=166, Percent_Identity=34.3373493975904, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355527, Length=160, Percent_Identity=33.75, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193 [H]

Pfam domain/function: PF00383 dCMP_cyt_deam_1 [H]

EC number: NA

Molecular weight: Translated: 18718; Mature: 18587

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHH
MALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDV
HHHHCCCEEEECCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQSSTD
HHCCCCCCEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWNRPIGRHDPTAHAEI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHH
MALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFGARDAKTGAAGSLMDV
HHHHCCCEEEECCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQSSTD
HHCCCCCCEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]