Click here to switch to the map view.

The map label for this gene is glcD [H]

Identifier: 170682557

GI number: 170682557

Start: 3334633

End: 3336123

Strand: Reverse

Name: glcD [H]

Synonym: EcSMS35_3257

Alternate gene names: 170682557

Gene position: 3336123-3334633 (Counterclockwise)

Preceding gene: 170683858

Following gene: 170682017

Centisome position: 65.82

GC content: 56.87

Gene sequence:

>1491_bases
TTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCC
TGGACTGGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCAT
TACTGGTTGTTCTGCCTAAGCAGATGGAACAGGTGACGGCGATTCTGGCTGTCTGCCACCGCCTGCGTGTACCGGTGGTG
ACCCGTGGTGCAGGCACCGGGCTTTCTGGTGGCGCGCTGCCGCTGGAAAAAGGTGTGTTGTTGGTGATGGCGCGCTTTAA
AGAGATCCTCGACATTAACCCCGTTGGTCGCCGTGCGCGCGTGCAGCCGGGCGTGCGTAACCTGGCGATCTCCCAGGCCG
TTGCGCCGCATAATCTCTATTACGCGCCGGATCCGTCCTCACAAATCGCCTGTTCGATTGGCGGCAATGTGGCGGAAAAT
GCCGGTGGCGTTCACTGCCTGAAATATGGTCTAACCGTACATAACCTGCTGAAAATTGAAGTGCAAACGCTGGACGGCGA
GGCGCTGACGCTGGGATCGGACGCGCTGGATTCGCCTGGTTTTGACCTGCTGGCGCTGTTTACCGGATCGGAAGGTATGC
TCGGCGTGACCACCGAAGTGACGGTAAAACTGCTGCCGAAGCCGCCCGTGGCGCGGGTGCTGTTAGCCAGCTTTGACTCG
GTAGAAAAAGCCGGACTTGCGGTTGGTGACATCATCGCCAATGGCATTATCCCCGGCGGGCTGGAGATGATGGATAACCT
GTCGATTCGCGCGGCGGAAGATTTTATTCATGCCGGTTATCCCGTCGATGCCGAAGCGATTTTGTTATGCGAACTGGACG
GCGTGGAGTCTGACGTACAGGAAGACTGCGAGCAGGTTAACGACATCTTGTTGAACGCGGGTGCGACTGACGTCCGTCTG
GCACAGGACGAAGCAGAGCGAGTACGTTTCTGGGCCGGTCGCAAAAATGCGTTCCCGGCGGTAGGACGTATCTCCCCGGA
TTACTACTGCATGGATGGCACCATCCCGCGCCGCGCCCTGCCTGGCGTACTGGAAGGCATTGCCCGTTTATCGCAGCAAT
ATGATTTACGCGTTGCCAACGTCTTTCATGCCGGAGACGGCAACATGCACCCGTTAATCCTTTTCGATGCCAACGAACCC
GGTGAATTTGCCCGCGCGGAAGAGCTGGGCGGGAAGATCCTCGAACTCTGCGTTGAAGTTGGCGGCAGCATCAGTGGGGA
ACATGGCATTGGGCGCGAAAAAATCAATCAAATGTGCGCCCAGTTCAACAGCGATGAAATCACGACCTTCCATGCGGTCA
AGGCGGCGTTTGACCCCGATGGTTTGCTGAACCCAGGAAAAAACATTCCCACGCTACACCGCTGTGCTGAATTTGGTGCC
ATGCATGTGCATCACGGTCATTTACCTTTTCCTGAACTGGAGCGTTTCTGA

Upstream 100 bases:

>100_bases
AGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGAAAAAATCAGCTGCCACACAACACAACAAAGC
GAACCCTACTCATGAGCATC

Downstream 100 bases:

>100_bases
TGCTACGCGAGTGTGATTACAGCCAGGCGCTGCTGGAGCAGGTGAATCAGGCGATTAGCGATAAAACGCCGCTGGTGATT
CAGGGCAGCAATAGCAAAGC

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVV
TRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAEN
AGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRL
AQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEP
GEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
MHVHHGHLPFPELERF

Sequences:

>Translated_496_residues
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVV
TRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAEN
AGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRL
AQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEP
GEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
MHVHHGHLPFPELERF
>Mature_496_residues
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVV
TRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAEN
AGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRL
AQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEP
GEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
MHVHHGHLPFPELERF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=425, Percent_Identity=30.3529411764706, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI37595754, Length=448, Percent_Identity=29.9107142857143, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI119964728, Length=469, Percent_Identity=28.7846481876333, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1789351, Length=496, Percent_Identity=99.3951612903226, Blast_Score=999, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=501, Percent_Identity=25.1497005988024, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71992373, Length=431, Percent_Identity=28.3062645011601, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71992381, Length=431, Percent_Identity=26.6821345707657, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71992392, Length=359, Percent_Identity=27.2980501392758, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17534361, Length=471, Percent_Identity=28.0254777070064, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320023, Length=453, Percent_Identity=29.1390728476821, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6320764, Length=475, Percent_Identity=27.3684210526316, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320027, Length=436, Percent_Identity=25.9174311926606, Blast_Score=156, Evalue=9e-39,
Organism=Drosophila melanogaster, GI18921117, Length=444, Percent_Identity=28.3783783783784, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639277, Length=444, Percent_Identity=28.3783783783784, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639275, Length=444, Percent_Identity=28.3783783783784, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24653753, Length=476, Percent_Identity=22.4789915966387, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53457; Mature: 53457

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPK
CCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEECCH
QMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRAR
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCCC
VQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIE
CCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEEEEE
VQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
EEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQ
HHHHCCHHHHHHHHCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCHHHH
EDCEQVNDILLNAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRAL
HHHHHHHHHEECCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHH
PGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELCVEV
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
MHVHHGHLPFPELERF
EEEECCCCCCHHHHCC
>Mature Secondary Structure
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPK
CCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEECCH
QMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRAR
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCCC
VQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIE
CCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEEEEE
VQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
EEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQ
HHHHCCHHHHHHHHCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCHHHH
EDCEQVNDILLNAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRAL
HHHHHHHHHEECCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHH
PGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELCVEV
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
MHVHHGHLPFPELERF
EEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]