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The map label for this gene is insB1 [H]

Identifier: 170682442

GI number: 170682442

Start: 2273207

End: 2273710

Strand: Reverse

Name: insB1 [H]

Synonym: EcSMS35_2252

Alternate gene names: 170682442

Gene position: 2273710-2273207 (Counterclockwise)

Preceding gene: 170680370

Following gene: 170683241

Centisome position: 44.86

GC content: 55.36

Gene sequence:

>504_bases
ATGCCGGGCAACCGCCCGCATTATGGGCGCTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAAT
CGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATG
GCGACGCTGGGGCGTCTTATGAGCCTGCTGTCACCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGA
ATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGC
AGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTAT
CTGAACATAAAACACTATCAATAA

Upstream 100 bases:

>100_bases
GCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTTGG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCTGCACCTCGCAGCCTTCAATTTCAGCCACAATTGCATCAATATCTTTACGCAACTGGTCGATTTT
GCTGACCGTGGTTTTCAGCT

Product: IS1 transposase orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MPGNRPHYGRWPQHDFPPFKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTM
ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQ

Sequences:

>Translated_167_residues
MPGNRPHYGRWPQHDFPPFKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTM
ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQ
>Mature_166_residues
PGNRPHYGRWPQHDFPPFKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMA
TLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYL
NIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1786470, Length=167, Percent_Identity=99.4011976047904, Blast_Score=346, Evalue=4e-97,
Organism=Escherichia coli, GI1786459, Length=167, Percent_Identity=99.4011976047904, Blast_Score=346, Evalue=4e-97,
Organism=Escherichia coli, GI1787223, Length=167, Percent_Identity=98.8023952095808, Blast_Score=344, Evalue=2e-96,
Organism=Escherichia coli, GI1789853, Length=167, Percent_Identity=97.0059880239521, Blast_Score=338, Evalue=1e-94,
Organism=Escherichia coli, GI1788203, Length=167, Percent_Identity=97.0059880239521, Blast_Score=338, Evalue=1e-94,
Organism=Escherichia coli, GI1786203, Length=167, Percent_Identity=97.0059880239521, Blast_Score=338, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 19743; Mature: 19612

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGNRPHYGRWPQHDFPPFKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PGNRPHYGRWPQHDFPPFKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]