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The map label for this gene is ghrA [H]

Identifier: 170682399

GI number: 170682399

Start: 2108472

End: 2109410

Strand: Reverse

Name: ghrA [H]

Synonym: EcSMS35_2100

Alternate gene names: 170682399

Gene position: 2109410-2108472 (Counterclockwise)

Preceding gene: 170684239

Following gene: 170682055

Centisome position: 41.62

GC content: 51.01

Gene sequence:

>939_bases
ATGGATATCATCTTTTATCACCCAACGTTTGATACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAG
AGTCAGAGCATGGAAAAGCGGAGATAATGTCTCTGCTGATTATGCTTTAGTCTGGCATCCTCCAGTTGAAATGCTGGCAG
GGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCAGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATG
CTGAAACCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGT
GCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTTCCTGAATATCATC
GGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTT
CCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATT
TCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACACCTGAAACCGTCGGCATTATTAATCAACAATTACTCG
AAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTCGTGGAAGATGACCTGCTCGCGGCGCTG
GATAGCGGCAAAGTTAAAGGCGCAATGCTGGATGTTTTTAATCGTGAACCCTTACCGCCGGAAAGTCCGCTCTGGCAACA
TCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGATTACATTTCGCGCACCATTG
CCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAA

Upstream 100 bases:

>100_bases
CTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCGC
AACGCTTTTCGGGAGTCAGT

Downstream 100 bases:

>100_bases
TAAAGCATCAGGTTTCCTGCTATCCTTGGCGGGAATTGAATACAGGAGAGAGTTATGTATCCCGTCGACCTTCATATGCA
TACCGTTGCCAGCACGCATG

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNVSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY

Sequences:

>Translated_312_residues
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNVSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY
>Mature_312_residues
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNVSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI23308577, Length=261, Percent_Identity=26.8199233716475, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI87081824, Length=312, Percent_Identity=99.0384615384615, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=196, Percent_Identity=28.0612244897959, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=309, Percent_Identity=26.8608414239482, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=25.9433962264151, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324980, Length=178, Percent_Identity=28.0898876404494, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324055, Length=130, Percent_Identity=33.0769230769231, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574286, Length=262, Percent_Identity=30.1526717557252, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45552429, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585514, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28574282, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45551003, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574284, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585516, Length=234, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921140, Length=157, Percent_Identity=31.2101910828025, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35328; Mature: 35328

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNVSADYALVWHPPVEMLAGRDLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHH
FALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQ
HHHHCCHHHHHHHHHHCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
QNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAG
CCCCCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
REELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVDYISRTIAQLEKGE
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
RVCGQVDRARGY
HHHHHHHHHCCC
>Mature Secondary Structure
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNVSADYALVWHPPVEMLAGRDLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHH
FALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQ
HHHHCCHHHHHHHHHHCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
QNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAG
CCCCCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
REELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVDYISRTIAQLEKGE
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
RVCGQVDRARGY
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA