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The map label for this gene is yahF [H]

Identifier: 170682351

GI number: 170682351

Start: 4301599

End: 4303158

Strand: Reverse

Name: yahF [H]

Synonym: EcSMS35_4201

Alternate gene names: 170682351

Gene position: 4303158-4301599 (Counterclockwise)

Preceding gene: 170684276

Following gene: 170680799

Centisome position: 84.9

GC content: 52.05

Gene sequence:

>1560_bases
ATGAAAGTTAAATATAAAGTTTTCAGTAATCTTTATCAGGACTCAGTTTCTCTGATGCAGATTTCCGCGCAAATCAGCAA
GTTGCCCGGTATTCAACAGGCCTCGGTCGTCATGGGAACACCCAATAATCTCGAGCAATTGAGCGATGCGGGTCTGGGTA
ACGAAATCAACGCCAGTCCCAATGATTTAGTGATTGCAGTCATGGGTGAAGAAGATATTTGCAATGAAGCACTGGTACTG
GCCCAGCAACGTCTGACCAGCAAACCCGATGATGAAACGGATAGTGGCATTAAAACACCGGAAAAGGTCAGCCTGGAAAT
GGCACTGGAAGCCGAGCCCGAAGCCAACCTGGCACTCATTTCTGTACCGGGAGACTACGCCGCCGCCGAAGCAATTAAAG
CGTTAAACCTCGGAATGAACGTGATGATGTTCAGCGATAACGTCAGTATCGTGCAGGAAAAATCCATCAAAACGCTTGCC
CGAGAACGTCAGCGCATCGTAATGGGACCTGACTGTGGAACCGCCATTGTTAACGGCATTCCGTTAGGCTTCGCTAATGT
GGTGAAACGCGGAGCAATTGGTGTTATTGGCGCGTCAGGCACCGGGCTACAGGAAGTGACTTGCCGAATCGACCAGCTCG
GAGCGGGTATTTCTCAGGCGTTGGGAACAGGCGGACATGATCTCAGTGAAGAGATTGGTGGAATATCGATGCTCTTCGCT
TTAGATGCGCTGGCGCAAGACGATGAAACTCGCGTGATTGTGCTTATTTCAAAACCGCCTTCACCGATCGTTGCCCGGAC
TATTCTGGAGCGTGCCGAGGCTTGTGGAAAACCCGTGGTGGTGAATTTCCTCGGTGCGAATCCGCATGATCTGGCACGTC
CCAATATCACCGCGGCCACCACACTTGCCAGCGCCGCAAATATCGCCGTGGCGTTACTGAATGATCAACCACTTCCGACT
ATCGAGACCGAAATTTCCTGCGACGATCTCACAATGTTACAAAACGCCTGCCAGCGCCTGCCAGCCCATCGCCAGGCGAT
ACGCGGTGTTTTCGCCGGAGGAACCTTCTGCTACGAAGCGCAACTTATCTGCCAACAAAAGGGATTCAGCGCAGCCTCCA
ATACGCCAGTTGCGGGTAATCGTGCTCTGGCAAATATCTGGCAAAGCGAAGATCACACGTTGATCGATATGGGCGACGAT
GATTTCACCCGTGGCAAACCGCATCCAATGATCGACCCGACTCTGCGCAATCAACGTCTGCTCAACGAACTCAACGACTC
ACACACTGCCGTCGTGTTATTCGACCTGGTACTTGGTTATGGCGCTTCCACGACGCCTGCCAGCGAATTACTGGACCAAC
TTTCACACATCGACATGAATAACGCACCGTTGCTGATTGCGCACGTTTGCGGAACGGAAGCTGATCCGCAAATACGCAGT
CAGCAGATAAGCGCACTACAAAATGCAGGGGTCATCATCGCCAGCAGCAATGCACAGGCGGCGTTATGGGCCAGCACCGT
TGCACAGACTCAGTTGCAGAAAAAGGGGCTAAACGCATGA

Upstream 100 bases:

>100_bases
AATTGATTTCAACCTTTTCTCCCGTAGCCCGTTTATGGGGCTACACGGGGATGCTTTGCCCGAATTTTGCTATCGGGCGG
AATGAAACAGGCGTAACAGC

Downstream 100 bases:

>100_bases
ATACCTTATTTAATCAGCCTTTGAAGGTTGTGAACGCGGGACTACACAGTTTTGCTGACAATATTCAACATGCCGGGGGA
AGCGCAATTGCACTCAACTG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNLEQLSDAGLGNEINASPNDLVIAVMGEEDICNEALVL
AQQRLTSKPDDETDSGIKTPEKVSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGISQALGTGGHDLSEEIGGISMLFA
LDALAQDDETRVIVLISKPPSPIVARTILERAEACGKPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPT
IETEISCDDLTMLQNACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDHTLIDMGDD
DFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASELLDQLSHIDMNNAPLLIAHVCGTEADPQIRS
QQISALQNAGVIIASSNAQAALWASTVAQTQLQKKGLNA

Sequences:

>Translated_519_residues
MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNLEQLSDAGLGNEINASPNDLVIAVMGEEDICNEALVL
AQQRLTSKPDDETDSGIKTPEKVSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGISQALGTGGHDLSEEIGGISMLFA
LDALAQDDETRVIVLISKPPSPIVARTILERAEACGKPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPT
IETEISCDDLTMLQNACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDHTLIDMGDD
DFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASELLDQLSHIDMNNAPLLIAHVCGTEADPQIRS
QQISALQNAGVIIASSNAQAALWASTVAQTQLQKKGLNA
>Mature_519_residues
MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNLEQLSDAGLGNEINASPNDLVIAVMGEEDICNEALVL
AQQRLTSKPDDETDSGIKTPEKVSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGISQALGTGGHDLSEEIGGISMLFA
LDALAQDDETRVIVLISKPPSPIVARTILERAEACGKPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPT
IETEISCDDLTMLQNACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDHTLIDMGDD
DFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASELLDQLSHIDMNNAPLLIAHVCGTEADPQIRS
QQISALQNAGVIIASSNAQAALWASTVAQTQLQKKGLNA

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli fdrA and some, to bacterial sucD [H]

Homologues:

Organism=Escherichia coli, GI1786513, Length=513, Percent_Identity=41.7153996101365, Blast_Score=354, Evalue=1e-98,
Organism=Escherichia coli, GI1786729, Length=504, Percent_Identity=40.4761904761905, Blast_Score=318, Evalue=4e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003781
- InterPro:   IPR005811
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: NA

Molecular weight: Translated: 54953; Mature: 54953

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNLEQLSDAGLGNEINASP
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCC
NDLVIAVMGEEDICNEALVLAQQRLTSKPDDETDSGIKTPEKVSLEMALEAEPEANLALI
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEE
SVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLARERQRIVMGPDCGTAIVNGI
ECCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHCCC
PLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGISQALGTGGHDLSEEIGGISMLFA
CCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCHHHHH
LDALAQDDETRVIVLISKPPSPIVARTILERAEACGKPVVVNFLGANPHDLARPNITAAT
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCHHHH
TLASAANIAVALLNDQPLPTIETEISCDDLTMLQNACQRLPAHRQAIRGVFAGGTFCYEA
HHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEHH
QLICQQKGFSAASNTPVAGNRALANIWQSEDHTLIDMGDDDFTRGKPHPMIDPTLRNQRL
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHH
LNELNDSHTAVVLFDLVLGYGASTTPASELLDQLSHIDMNNAPLLIAHVCGTEADPQIRS
HHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHH
QQISALQNAGVIIASSNAQAALWASTVAQTQLQKKGLNA
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNLEQLSDAGLGNEINASP
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCC
NDLVIAVMGEEDICNEALVLAQQRLTSKPDDETDSGIKTPEKVSLEMALEAEPEANLALI
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEE
SVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLARERQRIVMGPDCGTAIVNGI
ECCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHCCC
PLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGISQALGTGGHDLSEEIGGISMLFA
CCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCHHHHH
LDALAQDDETRVIVLISKPPSPIVARTILERAEACGKPVVVNFLGANPHDLARPNITAAT
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCHHHH
TLASAANIAVALLNDQPLPTIETEISCDDLTMLQNACQRLPAHRQAIRGVFAGGTFCYEA
HHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEHH
QLICQQKGFSAASNTPVAGNRALANIWQSEDHTLIDMGDDDFTRGKPHPMIDPTLRNQRL
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHH
LNELNDSHTAVVLFDLVLGYGASTTPASELLDQLSHIDMNNAPLLIAHVCGTEADPQIRS
HHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHH
QQISALQNAGVIIASSNAQAALWASTVAQTQLQKKGLNA
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]