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The map label for this gene is ybjI [H]

Identifier: 170682272

GI number: 170682272

Start: 877948

End: 878763

Strand: Reverse

Name: ybjI [H]

Synonym: EcSMS35_0871

Alternate gene names: 170682272

Gene position: 878763-877948 (Counterclockwise)

Preceding gene: 170679939

Following gene: 170682945

Centisome position: 17.34

GC content: 45.96

Gene sequence:

>816_bases
ATGAGCATTAAATTAATTGCGGTAGACATGGATGGTACTTTCTTAAGCGATCAAAAAACCTATAACCGTGAGCGGTTTAT
GGCTCAGTATCAGCAAATGAAAGCACAAGGCATTCGCTTTGTGGTCGCCAGCGGGAATCAATATTATCAGTTGATCTCTT
TCTTTCCGGAAATTGCTAATGAAATAGCCTTTGTGGCTGAAAACGGCGGCTGGGTAGTGAGCGAAGGCAAAGATGTTTTT
AATGGCGAGCTGTCGAAGGATGCGTTTGCTACTGTCGTGGAACATTTGCTGACGCGCCCGGAAGTGGAAATTATTGCCTG
CGGAAAAAATAGCGCCTATACCCTCAAAAAATATGACGATGCCATGAAAACGGTGGCGGAAATGTATTATCACCGTCTGG
AATACGTCGATAACTTTGACAACTTAGAAGATATCTTCTTTAAGTTTGGTCTGAATCTTTCCGATGAACTGATTCCACAA
GTACAAAAAGCATTACATGAGGCCATCGGCGATATTATGGTGCCGGTCCACACCGGCAACGGCAGCATCGATCTGATTAT
CCCCGGCGTACATAAAGCCAATGGCCTTCGCCAACTGCAGAAATTATGGGGAATAGACGACAGCGAAGTGGTGGTCTTTG
GCGATGGCGGTAACGATATTGAGATGCTGCGTCAGGCAGGCTTTAGTTTTGCAATGGAAAATGCCGGCAGCGCGGTCGTC
GCAGCAGCAAAATACCGGGCAGGCTCCAATAACCGTGAAGGCGTACTGGATGTGATCGATAAAGTTCTTAAACACGAAGC
GCCATTTAACCAATAA

Upstream 100 bases:

>100_bases
CAATGCTGGTTGTACTGGCGCTGGTTATTCTCGCGGCTATTGTCGCGAAAGCCGTCGCCAAACCCGATACCAAAACGCAG
ACGGCGATGGAGAATAGTTG

Downstream 100 bases:

>100_bases
AAACGGCAGCACGCCCGGCAACACAATGCCTGAATGAAGGCTGCATAATTCGCGGAAATTAACCCGCTGCATTATTTCAG
GCGAAATGATTATGAAAAAT

Product: phosphatase YbjI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVF
NGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
AAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ

Sequences:

>Translated_271_residues
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVF
NGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
AAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ
>Mature_270_residues
SIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFN
GELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQV
QKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVA
AAKYRAGSNNREGVLDVIDKVLKHEAPFNQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=271, Percent_Identity=99.2619926199262, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1787043, Length=270, Percent_Identity=52.2222222222222, Blast_Score=288, Evalue=2e-79,
Organism=Escherichia coli, GI2367265, Length=271, Percent_Identity=26.1992619926199, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30206; Mature: 30074

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIAN
CCEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDD
HEEEEECCCCEEEECCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHH
AMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
CEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHHHHCCCCEEE
AAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ
EEHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIAN
CEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDD
HEEEEECCCCEEEECCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHH
AMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
CEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHHHHCCCCEEE
AAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ
EEHHHCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]