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The map label for this gene is yafV [H]

Identifier: 170682248

GI number: 170682248

Start: 246450

End: 247220

Strand: Reverse

Name: yafV [H]

Synonym: EcSMS35_0231

Alternate gene names: 170682248

Gene position: 247220-246450 (Counterclockwise)

Preceding gene: 170681415

Following gene: 170682534

Centisome position: 4.88

GC content: 54.73

Gene sequence:

>771_bases
GTGCCTGGTTTGAAGATTACGCTTTTGCAGCAACCACTGGTGTGGATGGATGGCCCTGCCAACCTGCGTCATTTTGATCG
TCAACTGGAAGGTATTACCGGACGCGATGTGATCGTTCTACCGGAGATGTTTACCAGCGGCTTTGCCATGGAAGCGGCAG
CTGCGTCGCTAGCACAAGATGACGTAGTGAACTGGATGACAGCCAAAGCGCAGCAGTGCAATGCGCTGATTGCAGGCAGT
GTTGCATTACAAACGGAGTCTGGTTCGGTTAACCGCTTTTTGCTGGTTGAGCCGGGCGGCACGGTACATTTTTATGATAA
GCGTCATCTGTTCCGAATGGCAGATGAGCATCTGCATTATAAAGCGGGCAATGCGCGAGTAATTGTGGAATGGCGCGGCT
GGCGTATTTTGCCGCTGGTGTGCTACGACTTACGTTTTCCTGTGTGGTCGCGCAATCTCAACGATTATGACCTCGCCCTG
TACGTCGCCAACTGGCCTGCTCCGCGCTCTCTGCACTGGCAGGCATTGCTGACAGCCCGCGCGATTGAGAATCAGGCGTA
TGTGGCGGGATGCAATCGCGTCGGCAGCGATGGCAACGGCTGCCATTATCGCGGTGACAGCAGAGTGATTAATCCACAGG
GCGAGATTATCGCCACCGCCGACGCGCATCAGGCAACGCGCATTGATGCGGAGCTGTCGATGGTGGCGTTGCGGGAATAT
AGAGAAAAGTTTCCGGCATGGCGGGATGCGGATGAATTCACATTTCGTTAG

Upstream 100 bases:

>100_bases
TTCCAATATCAGCATTTGTTATCAATTTATTCTGCGCTAAGGCAACTGCCTGATAAGGTGTTAGAGTTATAAAACCCTTC
GCATAAAGGAGTTGTACACC

Downstream 100 bases:

>100_bases
AGGGTGAATATGGCTACGAGATAAGTTTCGTGGCTATTTTTCTCTGGAACTTAAACGACGAAAGCCCGAATCAGTAATGA
TTCGGGCTTTCTGAATATTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAAASLAQDDVVNWMTAKAQQCNALIAGS
VALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMVALREY
REKFPAWRDADEFTFR

Sequences:

>Translated_256_residues
MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAAASLAQDDVVNWMTAKAQQCNALIAGS
VALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMVALREY
REKFPAWRDADEFTFR
>Mature_255_residues
PGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAAASLAQDDVVNWMTAKAQQCNALIAGSV
ALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLALY
VANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMVALREYR
EKFPAWRDADEFTFR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=233, Percent_Identity=26.1802575107296, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786412, Length=253, Percent_Identity=98.8142292490119, Blast_Score=522, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 28914; Mature: 28783

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAAASLAQD
CCCCEEEEECCCEEEECCCCHHHHHHHHHCCCCCCCEEEECHHHCCCCHHHHHHHHHHHH
DVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHY
HHHHHHHHHHHHCCEEEECEEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHEEE
KAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLALYVANWPAPRSLHWQALLTAR
ECCCEEEEEEECCCEEEEEEEEEECCCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
AIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMVALREY
HHCCCEEEEECCCCCCCCCCCEECCCCEEECCCCCEEEECCCCCCEEECHHHHHHHHHHH
REKFPAWRDADEFTFR
HHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMFTSGFAMEAAAASLAQD
CCCEEEEECCCEEEECCCCHHHHHHHHHCCCCCCCEEEECHHHCCCCHHHHHHHHHHHH
DVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTVHFYDKRHLFRMADEHLHY
HHHHHHHHHHHHCCEEEECEEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHEEE
KAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLALYVANWPAPRSLHWQALLTAR
ECCCEEEEEEECCCEEEEEEEEEECCCHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
AIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIATADAHQATRIDAELSMVALREY
HHCCCEEEEECCCCCCCCCCCEECCCCEEECCCCCEEEECCCCCCEEECHHHHHHHHHHH
REKFPAWRDADEFTFR
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]