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The map label for this gene is phnM [H]

Identifier: 170682245

GI number: 170682245

Start: 4661579

End: 4662715

Strand: Reverse

Name: phnM [H]

Synonym: EcSMS35_4561

Alternate gene names: 170682245

Gene position: 4662715-4661579 (Counterclockwise)

Preceding gene: 170684197

Following gene: 170680468

Centisome position: 92.0

GC content: 61.39

Gene sequence:

>1137_bases
ATGATTATCAATAACGTTAAGCTGGTGCTGGAAAACGAGGTGGTGCACGGTTCGCTGGAGGTGCAGGATGGCGAAATCCG
CGCCTTTGCCGAAAGCCAGAGCCGCCTGCCAGAAGCGATGGATGGCGAAGGCGGCTGGCTGCTGCCGGGGCTGATTGAGC
TGCATACCGATAATCTGGATAAATTCTTCACCCCGCGCCCGAAGGTTGACTGGCCCGCCCACTCGGCGATGAGTAGCCAC
GACGCGCTGATGGTGGCGAGCGGCATCACCACCGTGCTGGACGCGGTGGCGATTGGCGACGTGCGCGACGGCGGCGATCG
GCTGGAGAATCTGGAGAAGATGATCAACGCCATCGAAGAGACGCAGAAACGCGGCGTCAACCGCGCCGAGCACCGTCTGC
ATCTGCGCTGCGAACTGCCGCATCACACCACGCTGCCGCTGTTTGAAAAACTGGTGCAGCGCGAACCGGTGACGCTGGTG
TCGCTGATGGATCACTCGCCGGGCCAGCGCCAGTTCGCCAATCGCGAGAAGTATCGCGAATATTATCAGGGCAAATATTC
CCTCACCGATGCGCAGATGCAGCAGTACGAAGAGGAACAGCTGGCGCTTGCCGCACGCTGGTCACAGCCTAATCGCGAAA
CTATCGCCGCAATGTGCCGCGCGCGACACATTGCCCTCGCCAGCCACGATGACGCCACGCACGCCCACGTTGCCGAATCC
CACCAGCTTGGCAGCGTGATCGCCGAATTTCCCACCACGTTCGAAGCGGCAGAAGCCTCGCGCAAGCATGGCATGAACGT
GCTGATGGGCGCGCCGAATATCGTGCGCGGCGGTTCGCACTCCGGCAACGTGGCGGCCAGTGAACTGGCGCAGCTTGGCC
TGCTGGATATCCTCTCTTCCGACTACTACCCCGCCAGCCTGCTCGATGCGGCATTTCGCGTCGCCGATGACGAGAGCAAC
CGCTTTACGCTGCCGCAGGCGGTGAAGCTGGTGACTAAAAATCCGGCGCAGGCGCTTAATCTTCAGGATCGCGGGGTGAT
TGGCGAGGGCAAACGCGCCGACCTGGTGCTGGCGCATCGCAAGGGCAACCACATTCATATCGACCACGTCTGGCGTCAGG
GTAAAAGGGTGTTCTGA

Upstream 100 bases:

>100_bases
GCGAAGCCAAAACCCGTGGCGCAGCCATCGTAGGCATCTTCCATGACGAAGCTGTACGTAATGACGTCGCCGACCGCCTG
CACCCAATGGGAGCCTCTTC

Downstream 100 bases:

>100_bases
TGACGGGAAAACTGATTTGGTTAATGGGGGCCTCTGGCTCCGGAAAAGACAGTCTGTTGACGGAACTCCGCCAGCGGGAA
CAAACTCAGCTACTGGTAGC

Product: phosphonate metabolism protein PhnM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAMCRARHIALASHDDATHAHVAES
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESN
RFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF

Sequences:

>Translated_378_residues
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAMCRARHIALASHDDATHAHVAES
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESN
RFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF
>Mature_378_residues
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAMCRARHIALASHDDATHAHVAES
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESN
RFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3454

COG function: function code P; Metal-dependent hydrolase involved in phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790533, Length=378, Percent_Identity=98.1481481481482, Blast_Score=768, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059
- InterPro:   IPR012696 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: NA

Molecular weight: Translated: 42046; Mature: 42046

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLD
CEECCEEEEEECCEEECEEEEECCCCCEEHHHHHCCCHHCCCCCCEECCCCEEEECCCHH
KFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEE
HHCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLVSLMDHSPGQRQFANREKYRE
HHHHCCCHHHCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
YYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAMCRARHIALASHDDATHAHVAES
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHH
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSS
HHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DYYPASLLDAAFRVADDESNRFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHR
CCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEEEC
KGNHIHIDHVWRQGKRVF
CCCEEEHHHHHCCCCCCC
>Mature Secondary Structure
MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLD
CEECCEEEEEECCEEECEEEEECCCCCEEHHHHHCCCHHCCCCCCEECCCCEEEECCCHH
KFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEE
HHCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLVSLMDHSPGQRQFANREKYRE
HHHHCCCHHHCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
YYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAMCRARHIALASHDDATHAHVAES
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHH
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSS
HHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DYYPASLLDAAFRVADDESNRFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHR
CCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEEEC
KGNHIHIDHVWRQGKRVF
CCCEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]