Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 170681866

GI number: 170681866

Start: 2463027

End: 2464382

Strand: Reverse

Name: menE [H]

Synonym: EcSMS35_2415

Alternate gene names: 170681866

Gene position: 2464382-2463027 (Counterclockwise)

Preceding gene: 170682552

Following gene: 170680382

Centisome position: 48.62

GC content: 56.64

Gene sequence:

>1356_bases
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAGCCATCGCCTTACGTCTTAATGACGAGCA
ACTTAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAATTAGCCTCCGGCTTTGTGGCGCAGGGCGTTGTTGAGGGCAGCG
GCGTGATGTTGCGGGCGTGGAATACGCCGCAAACGCTGCTCGCCTGGCTGGCGTTACTGCAATGCGGGGCGCGGGTGTTG
CCCGTGAACCCTCAGCTGCCGCAACCGTTGCTTGAAGAATTGCTGCCCAATCTGACGCTGCAATTTGCGCTGGTGCCGGA
TGGGGAAAACACGTTTCCGGCGTTAACGTCGCTGCACATTCAGCTGGTTGAAGGCGCACATGCCGCTGCGTGGCAGCCGA
CGCGTCTGTGCTCAATGACGTTGACCTCCGGCTCTACCGGTTTGCCGAAAGCCGCTGTTCATACTTATCAGGCCCATCTT
GCCAGTGCGCAAGGTGTGTTATCGCTGATACCGTTTGGCGAGCACGATGACTGGTTGCTCTCTTTACCGCTGTTTCACGT
CTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGTGCGCGGATGACGGTACGTGATAAACAGCCCCTGGAGCAAA
TGCTGGCAGGCTGTACTCACGCTTCACTGGTACCAACACAACTCTGGCGTTTGCTGGTTAACCGTAGTTCCGTTTCCCTG
AAAGCGGTTTTACTTGGCGGCGCGGCTATCCCGGTCGAGTTGACGGAACAGGCGCGCGAGCAGGGGATTCGTTGCTTTTG
CGGCTATGGTCTGACCGAGTTTGCCTCCACGGTGTGTGCGAAAGAAGCCGACGGTCTGGCAGACGTTGGTTCGCCGCTGC
CGGGTCGGGAAGTGAAAATCGTTAATGATGAAGTGTGGCTGCGGGCTGCCAGTATGGCAGAAGGTTACTGGCGTAACGGG
CAACGGGTTCCACTGGTTAATGACGAAGGCTGGTACGCCACGCGCGATCGCGGTGAGATGCATAATGGCAAGCTGACCAT
TGTCGGACGTTTAGACAATCTATTCTTCAGTGGCGGTGAGGGTATTCAGCCGGAAGAAGTCGAGCGCGTTATTGCTGCAC
ATCCTGCGGTTTTGCAGGTGTTTATCGTCCCCGTTGCCGACAAGGAGTTTGGTCATCGGCCGGTAGCGGTGGTGGAGTAT
GACCAGCAAACCGTTGATCTTGACGAATGGGTGAAAGACAAGTTGGCTCGTTTTCAACAACCGGTGCGCTGGCTGACTTT
GCCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGTCAGGCGTTAAAAGAGTGGGTGCAACGCCAGGATTAA

Upstream 100 bases:

>100_bases
CGGGGCTGGACACGCTGGATCTGATGCAGGCGCAGCAGGTTCGTCGCTGGCCGGGTAGCCCGCTACCGTTAGTGGAAGTT
GATGCACTGGAGCGGCTGTT

Downstream 100 bases:

>100_bases
GAAATCATTAGTTTCACTTCGCAATTTTGCCATTCCATTGCCGTTGATAATAGGTTTATCTGTTAATCTGTAAATTGATG
TGAAAACTCTTATCAAACAG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFVAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQD

Sequences:

>Translated_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFVAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQD
>Mature_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFVAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQTVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQD

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=22.5092250922509, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI122937307, Length=419, Percent_Identity=24.582338902148, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788595, Length=450, Percent_Identity=97.1111111111111, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=350, Percent_Identity=25.1428571428571, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI221142682, Length=494, Percent_Identity=23.4817813765182, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=23.2741617357002, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=26.4204545454545, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=25.5555555555556, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI32563687, Length=461, Percent_Identity=25.3796095444685, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=473, Percent_Identity=26.0042283298097, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=25.0501002004008, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=342, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=342, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=342, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=492, Percent_Identity=23.3739837398374, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=495, Percent_Identity=22.8282828282828, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=295, Percent_Identity=25.7627118644068, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25141359, Length=400, Percent_Identity=24, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71996755, Length=343, Percent_Identity=23.6151603498542, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581924, Length=526, Percent_Identity=22.6235741444867, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50180; Mature: 50180

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFVAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHCEECCCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHEE
QLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGEHDDWLL
EEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECHHHHEEHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQTVDLDEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQD
CCEEEECCHHHCCCCEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFVAQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHCEECCCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHEE
QLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGEHDDWLL
EEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECHHHHEEHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQTVDLDEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQD
CCEEEECCHHHCCCCEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]